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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
19/10/2012 |
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Data da última atualização: |
19/10/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMOS, C. A. N.; ARAUJO, F. R.; ALVES, L. C.; SOUZA, I. I. F. de; GUEDES JUNIOR, D. S.; SOARES, C. O. |
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Afiliação: |
CARLOS A.N. RAMOS, Bolsista CNPGC; FLABIO RIBEIRO ARAUJO, CNPGC; LEUCIO C. ALVES, Laboratório de Doenc¸ as Parasitárias dos Animais Domésticos, Departamento de Medicina Veterinária –UFRPE, Recife, PE; INGRID IEDA FERNANDO DE SOUZA, Bolsista CNPGC; DANIEL S. GUEDES JUNIOR, BolsistaCNPGC; CLEBER OLIVEIRA SOARES, CNPGC. |
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Título: |
Genetic conservation of potentially immunogenic proteins among Brazilian isolates of Babesia bovis. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 187, n. 3-4, p. 548-552, julho, 2012. |
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Idioma: |
Português |
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Conteúdo: |
Bovine babesiosis caused by Babesia bovis remains an important constraint for the development of cattle industries worldwide. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these vaccines have a number of drawbacks, which justifies the search for better, safer vaccines. In recent years, a number of parasite proteins with immunogenic potential have been discovered. However, there is little information on the genetic conservation of these proteins among different parasite isolates, which hinders their assessment as immunogens. The aim of the present study was to evaluate the conservation of the genes ama-1, acs-1, rap-1, trap, p0 and msa2c among five Brazilian isolates of B. bovis. Through polymerase chain reaction, genetic sequencing and bioinformatics analysis of the genes, a high degree of conservation (98?100%) was found among Brazilian isolates of B. bovis and the T2Bo isolate. Thus, these genes are worth considering as viable candidates to be included in a recombinant cocktail vaccine for cattle babesiosis caused by B. bovis. |
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Palavras-Chave: |
Genetic conservation; Vaccine. |
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Thesagro: |
Babesia bovis. |
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Thesaurus Nal: |
Brazil. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/68358/1/2012-Babesia-genoma.pdf
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Marc: |
LEADER 01793naa a2200229 a 4500
001 1937283
005 2012-10-19
008 2012 bl uuuu u00u1 u #d
100 1 $aRAMOS, C. A. N.
245 $aGenetic conservation of potentially immunogenic proteins among Brazilian isolates of Babesia bovis.$h[electronic resource]
260 $c2012
520 $aBovine babesiosis caused by Babesia bovis remains an important constraint for the development of cattle industries worldwide. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these vaccines have a number of drawbacks, which justifies the search for better, safer vaccines. In recent years, a number of parasite proteins with immunogenic potential have been discovered. However, there is little information on the genetic conservation of these proteins among different parasite isolates, which hinders their assessment as immunogens. The aim of the present study was to evaluate the conservation of the genes ama-1, acs-1, rap-1, trap, p0 and msa2c among five Brazilian isolates of B. bovis. Through polymerase chain reaction, genetic sequencing and bioinformatics analysis of the genes, a high degree of conservation (98?100%) was found among Brazilian isolates of B. bovis and the T2Bo isolate. Thus, these genes are worth considering as viable candidates to be included in a recombinant cocktail vaccine for cattle babesiosis caused by B. bovis.
650 $aBrazil
650 $aBabesia bovis
653 $aGenetic conservation
653 $aVaccine
700 1 $aARAUJO, F. R.
700 1 $aALVES, L. C.
700 1 $aSOUZA, I. I. F. de
700 1 $aGUEDES JUNIOR, D. S.
700 1 $aSOARES, C. O.
773 $tVeterinary Parasitology$gv. 187, n. 3-4, p. 548-552, julho, 2012.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
16/09/2008 |
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Data da última atualização: |
16/09/2008 |
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Autoria: |
CLUZEAU, D.; PÉRÈS, G.; MERCIER, V.; BISPO, A.; ARROUAYS, D.; WALTER, C.; CORTET, J.; VILLENAVE, C.; RUIZ, N.; RANJARD, L.; CHAUSSOD, R.; CANNAVACCIUOLO, M.; ROUGÉ, L.; JOLIVET, C.; FARGETTE, M.; MATEILLE, T.; LAVELLE, P.; LERMERCIER-FOUCAULT, B.; DUBS, F.; MARTIN-LAURENT, F.; VELASQUEZ, E.; BELLIDO, A.; GUERNION, M.; PONGE, J. F. |
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Título: |
Procedures and protocol for soil biodiversity monitoring: "RMQSBiodiv", a French Pilot area experience. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
In order to incorporate biological parameters into soil quality monitoring, a French program «
RMQS-biodiv » was performed at the regional scale (Brittany, France). This program assessed
a range of biological parameters, and was connected to another soil monitoring network
developed at the national scale (Soil Quality Measurement Network- RMQS) which monitor
agro-pedological parameters. The connexion of both programs allowed the merge of both biotic
and abiotic data in a larger dataset, the analysis and the monitoring of soil biodiversity (species
and functions) in relation to land use (mainly agricultural practices) and/or pedoclimatic
parameters.
The RMQS-Biodiv Program also focussed on the optimization of soil biota sampling procedures
with the objective of their necessary standardization at national or European levels. It finally
assessed emerging criteria with respect to their relevance vs their cost effectiveness before
promoting them towards practical field actors.
Thus, this programme required an important research network to measure a large range of
biological parameters: macrofauna (earthworms, total macrofauna), mesofauna (acarina and
collembola), microorganisms (nematodes, microbial biomass, bacterial and fungal diversity),
and also functional biological parameters (soil respiration, humus index).The pilot area covered
more than 27 000 km² and the sampling scheme followed a systematic approach based on a
16 X 16 km squared grid. 115 sites were sampled in 2006 and 2007. The sampling methods
adapted to the present study context were designed to meet, as far as possible, the ISO
standards.
This paper will expose the logistics required to perform this type of program. Moreover, the
protocols and procedures will be discussed with respect to the ISO standards and to the
propositions and recommendations made by the ENVASSO project (Environmental Assessment
of Soil for Monitoring). MenosIn order to incorporate biological parameters into soil quality monitoring, a French program «
RMQS-biodiv » was performed at the regional scale (Brittany, France). This program assessed
a range of biological parameters, and was connected to another soil monitoring network
developed at the national scale (Soil Quality Measurement Network- RMQS) which monitor
agro-pedological parameters. The connexion of both programs allowed the merge of both biotic
and abiotic data in a larger dataset, the analysis and the monitoring of soil biodiversity (species
and functions) in relation to land use (mainly agricultural practices) and/or pedoclimatic
parameters.
The RMQS-Biodiv Program also focussed on the optimization of soil biota sampling procedures
with the objective of their necessary standardization at national or European levels. It finally
assessed emerging criteria with respect to their relevance vs their cost effectiveness before
promoting them towards practical field actors.
Thus, this programme required an important research network to measure a large range of
biological parameters: macrofauna (earthworms, total macrofauna), mesofauna (acarina and
collembola), microorganisms (nematodes, microbial biomass, bacterial and fungal diversity),
and also functional biological parameters (soil respiration, humus index).The pilot area covered
more than 27 000 km² and the sampling scheme followed a systematic approach based on a
16 X 16 km squared grid. 115 sites were sampled in 2006 and... Mostrar Tudo |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03231naa a2200397 a 4500
001 1314823
005 2008-09-16
008 2008 bl uuuu u00u1 u #d
100 1 $aCLUZEAU, D.
245 $aProcedures and protocol for soil biodiversity monitoring$b"RMQSBiodiv", a French Pilot area experience.
260 $c2008
520 $aIn order to incorporate biological parameters into soil quality monitoring, a French program « RMQS-biodiv » was performed at the regional scale (Brittany, France). This program assessed a range of biological parameters, and was connected to another soil monitoring network developed at the national scale (Soil Quality Measurement Network- RMQS) which monitor agro-pedological parameters. The connexion of both programs allowed the merge of both biotic and abiotic data in a larger dataset, the analysis and the monitoring of soil biodiversity (species and functions) in relation to land use (mainly agricultural practices) and/or pedoclimatic parameters. The RMQS-Biodiv Program also focussed on the optimization of soil biota sampling procedures with the objective of their necessary standardization at national or European levels. It finally assessed emerging criteria with respect to their relevance vs their cost effectiveness before promoting them towards practical field actors. Thus, this programme required an important research network to measure a large range of biological parameters: macrofauna (earthworms, total macrofauna), mesofauna (acarina and collembola), microorganisms (nematodes, microbial biomass, bacterial and fungal diversity), and also functional biological parameters (soil respiration, humus index).The pilot area covered more than 27 000 km² and the sampling scheme followed a systematic approach based on a 16 X 16 km squared grid. 115 sites were sampled in 2006 and 2007. The sampling methods adapted to the present study context were designed to meet, as far as possible, the ISO standards. This paper will expose the logistics required to perform this type of program. Moreover, the protocols and procedures will be discussed with respect to the ISO standards and to the propositions and recommendations made by the ENVASSO project (Environmental Assessment of Soil for Monitoring).
700 1 $aPÉRÈS, G.
700 1 $aMERCIER, V.
700 1 $aBISPO, A.
700 1 $aARROUAYS, D.
700 1 $aWALTER, C.
700 1 $aCORTET, J.
700 1 $aVILLENAVE, C.
700 1 $aRUIZ, N.
700 1 $aRANJARD, L.
700 1 $aCHAUSSOD, R.
700 1 $aCANNAVACCIUOLO, M.
700 1 $aROUGÉ, L.
700 1 $aJOLIVET, C.
700 1 $aFARGETTE, M.
700 1 $aMATEILLE, T.
700 1 $aLAVELLE, P.
700 1 $aLERMERCIER-FOUCAULT, B.
700 1 $aDUBS, F.
700 1 $aMARTIN-LAURENT, F.
700 1 $aVELASQUEZ, E.
700 1 $aBELLIDO, A.
700 1 $aGUERNION, M.
700 1 $aPONGE, J. F.
773 $tIn: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.
Download
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