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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
02/08/2023 |
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Data da última atualização: |
26/10/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOMES, T. G.; FONSECA, F. C. de A.; ALVES, G. S. C.; SIQUEIRA, F. G. de; MILLER, R. N. G. |
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Afiliação: |
TAÍSA GODOY GOMES, Universidade de Brasília; FERNANDO CAMPOS DE ASSIS FONSECA, Universidade de Brasília; GABRIEL SERGIO COSTA ALVES, Universidade de Brasília; FELIX GONCALVES DE SIQUEIRA, CNPAE; ROBERT NEIL GERARD MILLER, Universidade de Brasília. |
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Título: |
Development of reference genes for RT-qPCR analysis of gene expression in Pleurotus pulmonarius for biotechnological applications. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Scientific Reports, v. 13, n. 12296, 2023. |
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DOI: |
https://doi.org/10.1038/s41598-023-39115-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Jatropha curcas is an oilseed crop with biorefinery applications. Whilst cake generated following oil extraction offers potential as a protein source for animal feed, inactivation of toxic phorbol esters present in the material is necessary. Pleurotus pulmonarius is a detoxifying agent for jatropha cake with additional potential as animal feed, edible mushroom and for enzyme production. For the characterization of fungal genes involved in phorbol ester degradation, together with other industrial applications, reverse transcription-quantitative PCR (RT-qPCR) is a tool that enables accurate quantification of gene expression. For this, reliable analysis requires reference genes for normalization of mRNA levels validated under conditions employed for target genes. The stability of potential reference genes ?-TUB, ACTIN, GAPDH, PHOS, EF1?, TRPHO, LAC, MNP3, MYP and VP were evaluated following growth of P. pulmonarius on toxic, non-toxic jatropha cake and a combined treatment, respectively. NormFinder and geNorm algorithms for expression stability analysis identified PHOS, EF1? and MNP3 as appropriate for normalizing gene expression. Reference gene combinations contrasting in ranking were compared following normalization of relative expression of the CHU_2040 gene, encoding an esterase enzyme potentially involved in phorbol ester degradation. The reference genes for P. pulmonarius will facilitate the elucidation of mechanisms involved in detoxification of phorbol esters as well as analysis of target genes for application in biorefinery models. MenosJatropha curcas is an oilseed crop with biorefinery applications. Whilst cake generated following oil extraction offers potential as a protein source for animal feed, inactivation of toxic phorbol esters present in the material is necessary. Pleurotus pulmonarius is a detoxifying agent for jatropha cake with additional potential as animal feed, edible mushroom and for enzyme production. For the characterization of fungal genes involved in phorbol ester degradation, together with other industrial applications, reverse transcription-quantitative PCR (RT-qPCR) is a tool that enables accurate quantification of gene expression. For this, reliable analysis requires reference genes for normalization of mRNA levels validated under conditions employed for target genes. The stability of potential reference genes ?-TUB, ACTIN, GAPDH, PHOS, EF1?, TRPHO, LAC, MNP3, MYP and VP were evaluated following growth of P. pulmonarius on toxic, non-toxic jatropha cake and a combined treatment, respectively. NormFinder and geNorm algorithms for expression stability analysis identified PHOS, EF1? and MNP3 as appropriate for normalizing gene expression. Reference gene combinations contrasting in ranking were compared following normalization of relative expression of the CHU_2040 gene, encoding an esterase enzyme potentially involved in phorbol ester degradation. The reference genes for P. pulmonarius will facilitate the elucidation of mechanisms involved in detoxification of phorbol esters as well as... Mostrar Tudo |
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Thesagro: |
Jatropha Curcas; Refinaria. |
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Thesaurus Nal: |
Oilseed crops. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1155580/1/Development-of-reference.pdf
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Marc: |
LEADER 02270naa a2200217 a 4500
001 2155580
005 2023-10-26
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41598-023-39115-4$2DOI
100 1 $aGOMES, T. G.
245 $aDevelopment of reference genes for RT-qPCR analysis of gene expression in Pleurotus pulmonarius for biotechnological applications.$h[electronic resource]
260 $c2023
520 $aJatropha curcas is an oilseed crop with biorefinery applications. Whilst cake generated following oil extraction offers potential as a protein source for animal feed, inactivation of toxic phorbol esters present in the material is necessary. Pleurotus pulmonarius is a detoxifying agent for jatropha cake with additional potential as animal feed, edible mushroom and for enzyme production. For the characterization of fungal genes involved in phorbol ester degradation, together with other industrial applications, reverse transcription-quantitative PCR (RT-qPCR) is a tool that enables accurate quantification of gene expression. For this, reliable analysis requires reference genes for normalization of mRNA levels validated under conditions employed for target genes. The stability of potential reference genes ?-TUB, ACTIN, GAPDH, PHOS, EF1?, TRPHO, LAC, MNP3, MYP and VP were evaluated following growth of P. pulmonarius on toxic, non-toxic jatropha cake and a combined treatment, respectively. NormFinder and geNorm algorithms for expression stability analysis identified PHOS, EF1? and MNP3 as appropriate for normalizing gene expression. Reference gene combinations contrasting in ranking were compared following normalization of relative expression of the CHU_2040 gene, encoding an esterase enzyme potentially involved in phorbol ester degradation. The reference genes for P. pulmonarius will facilitate the elucidation of mechanisms involved in detoxification of phorbol esters as well as analysis of target genes for application in biorefinery models.
650 $aOilseed crops
650 $aJatropha Curcas
650 $aRefinaria
700 1 $aFONSECA, F. C. de A.
700 1 $aALVES, G. S. C.
700 1 $aSIQUEIRA, F. G. de
700 1 $aMILLER, R. N. G.
773 $tScientific Reports$gv. 13, n. 12296, 2023.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Voltar
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria Tropical. Para informações adicionais entre em contato com cnpat.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
10/01/2022 |
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Data da última atualização: |
10/01/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AMARAL, J. B. C.; LOPES, F. B.; MAGALHÃES, A. C. M. de; KUJAWA, S.; TANIGUCHI, C. A. K.; TEIXEIRA, A. dos S.; LACERDA, C. F. de; QUEIROZ, T. R. G.; ANDRADE, E. M. de; ARAÚJO, I. C. da S.; NIEDBAŁA, G. |
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Afiliação: |
JULYANNE BRAGA CRUZ AMARAL, Department of Agricultural Engineering, Campus of PICI, Federal University of Ceará; FERNANDO BEZERRA LOPES, Department of Agricultural Engineering, Campus of PICI, Federal University of Ceará; ANA CAROLINE MESSIAS DE MAGALHÃES, Department of Soil Sciences, Federal University of Rio Grande do Sul; SEBASTIAN KUJAWA, Department of Biosystems Engineering, Faculty of Environmental and Mechanical Engineering, Pozna´n University of Life Sciences, Wojska Polskiego; CARLOS ALBERTO KENJI TANIGUCHI, CNPAT; ADUNIAS DOS SANTOS TEIXEIRA, Department of Agricultural Engineering, Campus of PICI, Federal University of Ceará; CLAUDIVAN FEITOSA DE LACERDA, Department of Agricultural Engineering, Campus of PICI, Federal University of Ceará; THALES RAFAEL GUIMARÃES QUEIROZ, Department of Agricultural Engineering, Campus of PICI, Federal University of Ceará; EUNICE MAIA DE ANDRADE, Department of Soil and Water Conservation, Federal Rural University of the Semi-Arid; ISABEL CRISTINA DA SILVA ARAÚJO, Department of Agricultural Engineering, Campus of PICI, Federal University of Ceará; GNIEWKO NIEDBAŁA, Department of Biosystems Engineering, Faculty of Environmental and Mechanical Engineering, Pozna´n University of Life Sciences, Wojska Polskiego. |
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Título: |
Quantifying nutrient content in the leaves of cowpea using remote sensing. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Applied Sciences, v. 12, 458, 2022. |
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DOI: |
https://doi.org/10.3390/app12010458 |
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Idioma: |
Inglês |
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Palavras-Chave: |
Avaliando o estado nutricional; Dados hiperespectrais; Evaluating nutritional status; Hyperspectral data. |
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Thesagro: |
Feijão. |
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Thesaurus NAL: |
Vigna unguiculata subsp. unguiculata. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00979naa a2200313 a 4500
001 2138951
005 2022-01-10
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/app12010458$2DOI
100 1 $aAMARAL, J. B. C.
245 $aQuantifying nutrient content in the leaves of cowpea using remote sensing.$h[electronic resource]
260 $c2022
650 $aVigna unguiculata subsp. unguiculata
650 $aFeijão
653 $aAvaliando o estado nutricional
653 $aDados hiperespectrais
653 $aEvaluating nutritional status
653 $aHyperspectral data
700 1 $aLOPES, F. B.
700 1 $aMAGALHÃES, A. C. M. de
700 1 $aKUJAWA, S.
700 1 $aTANIGUCHI, C. A. K.
700 1 $aTEIXEIRA, A. dos S.
700 1 $aLACERDA, C. F. de
700 1 $aQUEIROZ, T. R. G.
700 1 $aANDRADE, E. M. de
700 1 $aARAÚJO, I. C. da S.
700 1 $aNIEDBAŁA, G.
773 $tApplied Sciences$gv. 12, 458, 2022.
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Embrapa Agroindústria Tropical (CNPAT) |
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