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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
19/12/2018 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DIAS, A. T.; CASTRO, S. B. R. de; ALVES, C. C. de S.; EVANGELISTA, M. G.; SILVA, L. C. da; REIS, D. R. de L.; MACHADO, M. A.; JULIANO, M. A.; FERREIRA, A. P. |
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Afiliação: |
ALYRIA TEIXEIRA DIAS, UFJF; SANDRA BERTELLI RIBEIRO DE CASTRO, UFJF; CAIO CÉSAR DE SOUZA ALVES, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Teófilo Otoni; MARCILENE GOMES EVANGELISTA, UFJF; LUAN CRISTIAN DA SILVA, UFJF; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; MARCO ANTONIO MACHADO, CNPGL; MARIA APARECIDA JULIANO, Universidade Federal de São Paulo; ANA PAULA FERREIRA, UFJF. |
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Título: |
Genistein modulates the expression of Toll-like receptors in experimental autoimmune encephalomyelitis. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Inflammation Research, v. 67, n. 7, p. 597-608, 2018. |
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DOI: |
10.1007/s00011-018-1146-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract OBJECTIVE AND DESIGN: The present work investigates the modulation of experimental autoimmune encephalomyelitis (EAE) using genistein before the EAE induction. MATERIAL: Female C57BL/6 mice (n = 96 mice/experiment), 4-6 weeks old, were used to induce the EAE. The mice were divided into three experimental groups: non-immunized group, immunized group (EAE), and immunized and treated with genistein group (Genistein). TREATMENT: Genistein was used at a dose of 200 mg/kg s.c. and were initiated 2 days before the immunization and continued daily until day 6 postimmunization. METHODS: Animals were monitored daily for clinical signs of EAE up to day 21. Inflammatory infiltration, demyelination, Toll-like receptor (TLR) expression, cytokines and transcription factors were analyzed in spinal cords. RESULTS: The present study demonstrates, for the first time, the genistein ability to modulate the factors involved in the innate immune response in the early stages of EAE. The genistein therapy delayed the onset of the disease, with reduced inflammatory infiltration and demyelination. In addition, the expression of TLR3, TLR9 and IFN-β were increased in genistein group, with reduction in the factors of TH1 and Th17 cells. CONCLUSION: These findings shed light on the potential of genistein as a prophylactic strategy for multiple sclerosis (MS) prevention. |
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Palavras-Chave: |
Experimental autoimmune encephalomyelitis; IFN-beta; Multiple sclerosis; Toll-like. |
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Thesaurus Nal: |
Immune response. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02278naa a2200289 a 4500
001 2102082
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1007/s00011-018-1146-7$2DOI
100 1 $aDIAS, A. T.
245 $aGenistein modulates the expression of Toll-like receptors in experimental autoimmune encephalomyelitis.$h[electronic resource]
260 $c2018
520 $aAbstract OBJECTIVE AND DESIGN: The present work investigates the modulation of experimental autoimmune encephalomyelitis (EAE) using genistein before the EAE induction. MATERIAL: Female C57BL/6 mice (n = 96 mice/experiment), 4-6 weeks old, were used to induce the EAE. The mice were divided into three experimental groups: non-immunized group, immunized group (EAE), and immunized and treated with genistein group (Genistein). TREATMENT: Genistein was used at a dose of 200 mg/kg s.c. and were initiated 2 days before the immunization and continued daily until day 6 postimmunization. METHODS: Animals were monitored daily for clinical signs of EAE up to day 21. Inflammatory infiltration, demyelination, Toll-like receptor (TLR) expression, cytokines and transcription factors were analyzed in spinal cords. RESULTS: The present study demonstrates, for the first time, the genistein ability to modulate the factors involved in the innate immune response in the early stages of EAE. The genistein therapy delayed the onset of the disease, with reduced inflammatory infiltration and demyelination. In addition, the expression of TLR3, TLR9 and IFN-β were increased in genistein group, with reduction in the factors of TH1 and Th17 cells. CONCLUSION: These findings shed light on the potential of genistein as a prophylactic strategy for multiple sclerosis (MS) prevention.
650 $aImmune response
653 $aExperimental autoimmune encephalomyelitis
653 $aIFN-beta
653 $aMultiple sclerosis
653 $aToll-like
700 1 $aCASTRO, S. B. R. de
700 1 $aALVES, C. C. de S.
700 1 $aEVANGELISTA, M. G.
700 1 $aSILVA, L. C. da
700 1 $aREIS, D. R. de L.
700 1 $aMACHADO, M. A.
700 1 $aJULIANO, M. A.
700 1 $aFERREIRA, A. P.
773 $tInflammation Research$gv. 67, n. 7, p. 597-608, 2018.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
28/01/2021 |
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Data da última atualização: |
10/06/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ARAÚJO, F. C. B. das; CUNHA, E. F. M.; RODRIGUES, S. de M.; BONFIM, K.; TAVARES, E. J. M.; MOURA, M. F.; SILVA, G. F. da. |
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Afiliação: |
Francisca Chagas Bezerra das Araújo, UFRA; ELISA FERREIRA MOURA CUNHA, CPATU; SIMONE DE MIRANDA RODRIGUES, CPATU; KENNY BONFIM DE ARRUDA CARVALHO, Cenargen; ERALDO JOSE MADUREIRA TAVARES, CPATSA; Mônika Fecury Moura, CPATU; GILVAN FERREIRA DA SILVA, CPAA. |
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Título: |
Identification and validation of SNPs in the phytoene synthase 2 (psy2) gene associated with yellow color of the root in cassava (Manihot esculenta Crantz) accessions of the Brazilian Amazon. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Genetic Resources and Crop Evolution, v. 68, p. 1809-1824, 2021. |
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DOI: |
https://doi.org/10.1007/s10722-020-01097-x |
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Idioma: |
Inglês |
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Conteúdo: |
The biofortification of cassava (Manihot esculenta Crantz) roots is one of the actual goals of genetic breeding, with the development of roots richer in carotenoids. In order to evaluate the natural genetic variation of cassava, this study analyzed 78 accessions from the Brazilian Amazon to verify the presence of single nucleotide polymorphism (SNP) molecular markers in the phytoene synthase 2 (psy2) gene associated with root color. From the sequencing of 294 bp containing part of the intron 2 and the exon 3 of the psy2 gene, three SNPs located in the coding region and the existence of eight haplotypes were identified. The c.1434G > C (SNP1) and c.1485C > A (SNP3) variations were found in yellow and cream root accessions and nucleotide polymorphism resulted in amino acid substitution. SNP2 (c.1462T > C) was identified in accessions with white roots and its allelic variation did not result in amino acid substitution. Four haplotypes that presented SNP3 were widely distributed in the states of Pará, Amapá, Rondônia and Amazonas. All accessions of yellow or cream root that presented the SNP3 were in heterozygosity, confirming the dominance of the SNP, except for one accession, that could be used in genetic breeding. The effect of mutations on the structure and function of PSY2 was in silico evaluated by molecular dynamics and it was indicated that the aminoacid substitutions affected the active site of the protein depending on the distance. The results were compatible with the phenotype detected experimentally. MenosThe biofortification of cassava (Manihot esculenta Crantz) roots is one of the actual goals of genetic breeding, with the development of roots richer in carotenoids. In order to evaluate the natural genetic variation of cassava, this study analyzed 78 accessions from the Brazilian Amazon to verify the presence of single nucleotide polymorphism (SNP) molecular markers in the phytoene synthase 2 (psy2) gene associated with root color. From the sequencing of 294 bp containing part of the intron 2 and the exon 3 of the psy2 gene, three SNPs located in the coding region and the existence of eight haplotypes were identified. The c.1434G > C (SNP1) and c.1485C > A (SNP3) variations were found in yellow and cream root accessions and nucleotide polymorphism resulted in amino acid substitution. SNP2 (c.1462T > C) was identified in accessions with white roots and its allelic variation did not result in amino acid substitution. Four haplotypes that presented SNP3 were widely distributed in the states of Pará, Amapá, Rondônia and Amazonas. All accessions of yellow or cream root that presented the SNP3 were in heterozygosity, confirming the dominance of the SNP, except for one accession, that could be used in genetic breeding. The effect of mutations on the structure and function of PSY2 was in silico evaluated by molecular dynamics and it was indicated that the aminoacid substitutions affected the active site of the protein depending on the distance. T... Mostrar Tudo |
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Palavras-Chave: |
Biofortificação; Raíz rica em carotenoide. |
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Thesagro: |
Carotenóide; Mandioca; Manihot Esculenta; Polimorfismo; Proteína; Raiz Alimentícia; Variação Genética. |
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Thesaurus NAL: |
Carotenoids; Cassava; Haplotypes; Polymorphism. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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Marc: |
LEADER 02740naa a2200361 a 4500
001 2129648
005 2021-06-10
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s10722-020-01097-x$2DOI
100 1 $aARAÚJO, F. C. B. das
245 $aIdentification and validation of SNPs in the phytoene synthase 2 (psy2) gene associated with yellow color of the root in cassava (Manihot esculenta Crantz) accessions of the Brazilian Amazon.$h[electronic resource]
260 $c2021
520 $aThe biofortification of cassava (Manihot esculenta Crantz) roots is one of the actual goals of genetic breeding, with the development of roots richer in carotenoids. In order to evaluate the natural genetic variation of cassava, this study analyzed 78 accessions from the Brazilian Amazon to verify the presence of single nucleotide polymorphism (SNP) molecular markers in the phytoene synthase 2 (psy2) gene associated with root color. From the sequencing of 294 bp containing part of the intron 2 and the exon 3 of the psy2 gene, three SNPs located in the coding region and the existence of eight haplotypes were identified. The c.1434G > C (SNP1) and c.1485C > A (SNP3) variations were found in yellow and cream root accessions and nucleotide polymorphism resulted in amino acid substitution. SNP2 (c.1462T > C) was identified in accessions with white roots and its allelic variation did not result in amino acid substitution. Four haplotypes that presented SNP3 were widely distributed in the states of Pará, Amapá, Rondônia and Amazonas. All accessions of yellow or cream root that presented the SNP3 were in heterozygosity, confirming the dominance of the SNP, except for one accession, that could be used in genetic breeding. The effect of mutations on the structure and function of PSY2 was in silico evaluated by molecular dynamics and it was indicated that the aminoacid substitutions affected the active site of the protein depending on the distance. The results were compatible with the phenotype detected experimentally.
650 $aCarotenoids
650 $aCassava
650 $aHaplotypes
650 $aPolymorphism
650 $aCarotenóide
650 $aMandioca
650 $aManihot Esculenta
650 $aPolimorfismo
650 $aProteína
650 $aRaiz Alimentícia
650 $aVariação Genética
653 $aBiofortificação
653 $aRaíz rica em carotenoide
700 1 $aCUNHA, E. F. M.
700 1 $aRODRIGUES, S. de M.
700 1 $aBONFIM, K.
700 1 $aTAVARES, E. J. M.
700 1 $aMOURA, M. F.
700 1 $aSILVA, G. F. da
773 $tGenetic Resources and Crop Evolution$gv. 68, p. 1809-1824, 2021.
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