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Registro Completo |
Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
27/01/1997 |
Data da última atualização: |
03/04/2017 |
Autoria: |
ALVES, B. J. R.; BODDEY, R. M.; URQUIAGA, S. S. |
Título: |
A rapid and sensitive flow injection technique for the analysis of ammonium in soil extracts. |
Ano de publicação: |
1993 |
Fonte/Imprenta: |
Communications in Soil Science and Plant Analysis, v.24, n.3/4, p.277-284, 1993. |
Idioma: |
Inglês |
Conteúdo: |
The steam distilation technique for the quantification of soil mineral nitrogens is not well adapted to estimate very low levels of NH4+ and NO3- such as those found in tropical pastures. In this paper we describe a flow injection analysis (FIA) technique for the direct estimation of soil ammonium in potassium chloride extracts. The technique is based on the salicylate/hypochloride colorimetric reaction with the resulting colouration read at 647 nm. The speed of the reaction was increased by immersing the reaction coils in a water bath maintained at 70C. The technique was capable of accurately quantifying ammonium to concentrations as low as 0.05 mg NH4+-N/L (equivalent to 0.25 mg NH4+-N/g dry soil) and sample thoughputs of at least 60 samples per hour could be achieved. It was found that amino acids whould also give a positive colorimetric reaction but that in the ten tropical soils examined this was not a significant source of error. |
Palavras-Chave: |
Ammonium; Amonio; Analysis. |
Thesagro: |
Análise; Nitrogênio; Nutrição; Solo. |
Thesaurus Nal: |
nitrogen; nutrition; soil. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01656naa a2200265 a 4500 001 1790324 005 2017-04-03 008 1993 bl --- 0-- u #d 100 1 $aALVES, B. J. R. 245 $aA rapid and sensitive flow injection technique for the analysis of ammonium in soil extracts. 260 $c1993 520 $aThe steam distilation technique for the quantification of soil mineral nitrogens is not well adapted to estimate very low levels of NH4+ and NO3- such as those found in tropical pastures. In this paper we describe a flow injection analysis (FIA) technique for the direct estimation of soil ammonium in potassium chloride extracts. The technique is based on the salicylate/hypochloride colorimetric reaction with the resulting colouration read at 647 nm. The speed of the reaction was increased by immersing the reaction coils in a water bath maintained at 70C. The technique was capable of accurately quantifying ammonium to concentrations as low as 0.05 mg NH4+-N/L (equivalent to 0.25 mg NH4+-N/g dry soil) and sample thoughputs of at least 60 samples per hour could be achieved. It was found that amino acids whould also give a positive colorimetric reaction but that in the ten tropical soils examined this was not a significant source of error. 650 $anitrogen 650 $anutrition 650 $asoil 650 $aAnálise 650 $aNitrogênio 650 $aNutrição 650 $aSolo 653 $aAmmonium 653 $aAmonio 653 $aAnalysis 700 1 $aBODDEY, R. M. 700 1 $aURQUIAGA, S. S. 773 $tCommunications in Soil Science and Plant Analysis$gv.24, n.3/4, p.277-284, 1993.
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