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Registro Completo |
Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
Data corrente: |
07/02/2013 |
Data da última atualização: |
21/11/2013 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BORBA, R. S. de; SILVA, E. L. da; PONZETTO, J. M.; POZZOBON, A. P. B.; CENTOFANTE, L.; ALVES, A. L.; PARISE-MALTEMPI, P. P. |
Afiliação: |
RAFAEL SPLENDORE DE BORBA, UNESP, Rio Claro-SP; EDSON LOURENÇO DA SILVA, UNESP, Rio Claro-SP; JOSI MARGARETE PONZETTO, UNESP, Rio Claro-SP; ALLAN PIERRE BONETTI POZZOBON, UNESP, Rio Claro-SP; LIANO CENTOFANTE, UFMT; ANDERSON LUIS ALVES, CNPASA; PATRÍCIA PASQUALI PARISE-MALTEMPI, UNESP, Rio Claro-SP. |
Título: |
Genetic structure of the ornamental tetra fish species Piabucus melanostomus Holmberg, 1891 (CHARACIDAE, IGUANODECTINAE) in the Brazilian Pantanal wetlands inferred by mitochondrial DNA sequences. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Biota Neotropica, São Paulo, v. 13, n. 1, p. 42-46, 2013. |
ISSN: |
1676-0603 |
Idioma: |
Inglês |
Conteúdo: |
The subfamily Iguanodectinae comprises a group of small Neotropical fishes composed by two genera and 11 nominal species widely distributed in the Atlantic drainages of South America. Piabucus is the only genus of Iguanodectinae found in the Paraguay River basin, especially in the Pantanal of Mato Grosso State, where it is represented by Piabucus melanostomus. Given the wide distribution and the low dispersion capacity of this species, due the ecological constraints, it is possible that many interesting genetic features could be found in different populations. In this way, the aim of his work was to perform the phylogeographic pattern of P. melanostomus populations using mitochondrial DNA sequences. A total of 13 individuals from three rivers belonging the Mato Grosso wetland were sampled. The ATP sintetase (subunits 6 and 8) gene was completely sequenced, the mean of nucleotide base composition in the sequences was 31.2% (T), 30.2% (C), 26.9% (A) and 11.9% (G), with no gene saturation. The population analysis in the TCS program generated a network with six haplotypes (A to F), where the ancestral haplotype (A) has a frequency of 25% and is composed by individuals from Cuiabá and Paraguay Rivers. The phylogenetic analysis showed the occurrence of two mtDNA lineages (1 and 2), the distance observed between the two lineages was 0.6%. The phylogenetic and phylogeographic results as well as the negative values of Fst for some populations, indicate a possible occurrence of gene flow among the analyzed populations. These results highlights the importance of flood pulse existent on wetland as a vehicle that permits a temporary connection among isolated population maintaining the species genetic variability. MenosThe subfamily Iguanodectinae comprises a group of small Neotropical fishes composed by two genera and 11 nominal species widely distributed in the Atlantic drainages of South America. Piabucus is the only genus of Iguanodectinae found in the Paraguay River basin, especially in the Pantanal of Mato Grosso State, where it is represented by Piabucus melanostomus. Given the wide distribution and the low dispersion capacity of this species, due the ecological constraints, it is possible that many interesting genetic features could be found in different populations. In this way, the aim of his work was to perform the phylogeographic pattern of P. melanostomus populations using mitochondrial DNA sequences. A total of 13 individuals from three rivers belonging the Mato Grosso wetland were sampled. The ATP sintetase (subunits 6 and 8) gene was completely sequenced, the mean of nucleotide base composition in the sequences was 31.2% (T), 30.2% (C), 26.9% (A) and 11.9% (G), with no gene saturation. The population analysis in the TCS program generated a network with six haplotypes (A to F), where the ancestral haplotype (A) has a frequency of 25% and is composed by individuals from Cuiabá and Paraguay Rivers. The phylogenetic analysis showed the occurrence of two mtDNA lineages (1 and 2), the distance observed between the two lineages was 0.6%. The phylogenetic and phylogeographic results as well as the negative values of Fst for some populations, indicate a possible occurrence of gene f... Mostrar Tudo |
Thesagro: |
Genética; Mitocondria; Peixe ornamental. |
Thesaurus Nal: |
Genetics; Mitochondrial DNA; Ornamental fish; Pantanal. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/92818/1/Piabucus.pdf
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Marc: |
LEADER 02660naa a2200289 a 4500 001 1948641 005 2013-11-21 008 2013 bl uuuu u00u1 u #d 022 $a1676-0603 100 1 $aBORBA, R. S. de 245 $aGenetic structure of the ornamental tetra fish species Piabucus melanostomus Holmberg, 1891 (CHARACIDAE, IGUANODECTINAE) in the Brazilian Pantanal wetlands inferred by mitochondrial DNA sequences.$h[electronic resource] 260 $c2013 520 $aThe subfamily Iguanodectinae comprises a group of small Neotropical fishes composed by two genera and 11 nominal species widely distributed in the Atlantic drainages of South America. Piabucus is the only genus of Iguanodectinae found in the Paraguay River basin, especially in the Pantanal of Mato Grosso State, where it is represented by Piabucus melanostomus. Given the wide distribution and the low dispersion capacity of this species, due the ecological constraints, it is possible that many interesting genetic features could be found in different populations. In this way, the aim of his work was to perform the phylogeographic pattern of P. melanostomus populations using mitochondrial DNA sequences. A total of 13 individuals from three rivers belonging the Mato Grosso wetland were sampled. The ATP sintetase (subunits 6 and 8) gene was completely sequenced, the mean of nucleotide base composition in the sequences was 31.2% (T), 30.2% (C), 26.9% (A) and 11.9% (G), with no gene saturation. The population analysis in the TCS program generated a network with six haplotypes (A to F), where the ancestral haplotype (A) has a frequency of 25% and is composed by individuals from Cuiabá and Paraguay Rivers. The phylogenetic analysis showed the occurrence of two mtDNA lineages (1 and 2), the distance observed between the two lineages was 0.6%. The phylogenetic and phylogeographic results as well as the negative values of Fst for some populations, indicate a possible occurrence of gene flow among the analyzed populations. These results highlights the importance of flood pulse existent on wetland as a vehicle that permits a temporary connection among isolated population maintaining the species genetic variability. 650 $aGenetics 650 $aMitochondrial DNA 650 $aOrnamental fish 650 $aPantanal 650 $aGenética 650 $aMitocondria 650 $aPeixe ornamental 700 1 $aSILVA, E. L. da 700 1 $aPONZETTO, J. M. 700 1 $aPOZZOBON, A. P. B. 700 1 $aCENTOFANTE, L. 700 1 $aALVES, A. L. 700 1 $aPARISE-MALTEMPI, P. P. 773 $tBiota Neotropica, São Paulo$gv. 13, n. 1, p. 42-46, 2013.
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Embrapa Pesca e Aquicultura (CNPASA) |
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Registro Completo
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
16/03/2011 |
Data da última atualização: |
20/03/2012 |
Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
Autoria: |
MACIEL, V. F. A.; PACHECO, T. F.; GONCALVES, S. B. |
Afiliação: |
VICTOR FORTUNA ALVES MACIEL, UnB; THALYTA FRAGA PACHECO, CNPAE; SILVIA BELEM GONCALVES, CNPAE. |
Título: |
Padronização do uso de corante rodamina B para avaliação de atividade lipolítica em estirpes fúngicas. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Brasília, DF: Embrapa Agroenergia, 2010. |
Páginas: |
4 p. |
Descrição Física: |
il., color. |
Série: |
(Embrapa Agroenergia. Comunicado Técnico, 005). |
ISSN: |
2177-4447 |
Idioma: |
Português |
Palavras-Chave: |
Atividades lipolíticas; Corante rodamina B. |
Thesagro: |
Enzima; Fungo. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/30138/1/cot-05.pdf
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Marc: |
LEADER 00646nam a2200205 a 4500 001 1881013 005 2012-03-20 008 2010 bl uuuu u0uu1 u #d 022 $a2177-4447 100 1 $aMACIEL, V. F. A. 245 $aPadronização do uso de corante rodamina B para avaliação de atividade lipolítica em estirpes fúngicas. 260 $aBrasília, DF: Embrapa Agroenergia$c2010 300 $a4 p.$cil., color. 490 $a(Embrapa Agroenergia. Comunicado Técnico, 005). 650 $aEnzima 650 $aFungo 653 $aAtividades lipolíticas 653 $aCorante rodamina B 700 1 $aPACHECO, T. F. 700 1 $aGONCALVES, S. B.
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