Data/hora: 05/06/2024 03:46:30

Biblioteca(s): | Embrapa Suínos e Aves.
Data corrente: | 18/12/2017
Data da última atualização: | 18/06/2019
Autoria: | HE, Y.; MATHIEU, J.; YANG, Y.; YU, P.; SILVA, M. L. B. da; ALVAREZ, P. J. J.
Afiliação: | YA HE, Rice University Houston - Texas; JACQUES MATHIEU, Rice University Houston - Texas; YU YANG, Rice University Houston - Texas; PINGFENG YU, Rice University Houston - Texas; MARCIO LUIS BUSI DA SILVA, CNPSA; PEDRO J. J. ALVAREZ, Rice University Houston - Texas.
Título: | Dioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase.
Ano de publicação: | 2017
Fonte/Imprenta: | Environmental Science & Technology, v. 4, n. 11, p. 494-499, 2017.
DOI: | 10.1021/acs.estlett.7b00456
Idioma: | Inglês
Conteúdo: | ABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000&#8722;4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites and minimize false negatives.
Palavras-Chave: | Biodegradação de dioxano; Microbactéria.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/198674/1/marcio-busi.pdf
Registro original: | Embrapa Suínos e Aves (CNPSA)
Exemplares: | CNPSA - (21242-1) - UPC - AP - DD -  -  -  - DIS