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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Agroindústria Tropical; Embrapa Agropecuária Oeste; Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Arroz e Feijão; Embrapa Caprinos e Ovinos; Embrapa Cerrados; Embrapa Clima Temperado; Embrapa Gado de Corte; Embrapa Gado de Leite; Embrapa Pantanal; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Roraima; Embrapa Semiárido; Embrapa Trigo; Embrapa Unidades Centrais. MenosEmbrapa Agrobiologia; Embrapa Agroindústria Tropical; Embrapa Agropecuária Oeste; Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Arroz e Feijão; Embrapa Caprinos e Ovinos; Embrapa Cerrados; Embrapa Clima Temperado... Mostrar Todas |
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Data corrente: |
22/10/1999 |
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Data da última atualização: |
24/09/2014 |
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Autoria: |
ALTHOFF, M. A. |
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Título: |
Metodologias para conservacao de germoplasma-semente de leguminosas forrageiras. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Brasilia: Embrapa Recursos Geneticos e Biotecnologia, 2000 |
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Páginas: |
24p. |
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Série: |
(Embrapa Recursos Geneticos e Biotecnologia. Documentos, 45) |
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Idioma: |
Português |
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Conteúdo: |
Procedimentos adotados no laboratório de sementes da Embrapa Recursos Genéticos e Biotecnologia para a conservação a médio e longo prazo de sementes de leguminosas forrageiras, para possibilitar que os destinatários de amostras de sementes destas plantas tenham conhecimento da metodologia utilizada, e que os mesmos possam aplicá-la dentro das suas necessidades e possibilidades, ara que este germoplasma seja devidamente manejado, conservado e utilizado, minimizando-se perdas que podem ser irreparáveis. |
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Palavras-Chave: |
Appropriate technology; Armazenagem; BAG; Brasil; Brasilia; Conservacao de germoplasma; Conservacao de semente; Conservação do germoplasma; Conservation; Feed crops; Feed legumes; Forrageira; Germplas; Leguminosa forrrageira; Methods; Metodologia; Planta leguminosa forrageira; Seed; Semen preservation. |
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Thesagro: |
Conservação; Germoplasma; Legume; Leguminosa; Leguminosa Forrageira; Leguminosae; Regeneração; Semente; Tecnologia Apropriada. |
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Thesaurus Nal: |
forage; germplasm; germplasm conservation; seeds; storage. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01945nam a2200529 a 4500
001 1179945
005 2014-09-24
008 2000 bl uuuu u0uu1 u #d
100 1 $aALTHOFF, M. A.
245 $aMetodologias para conservacao de germoplasma-semente de leguminosas forrageiras.
260 $aBrasilia: Embrapa Recursos Geneticos e Biotecnologia$c2000
300 $a24p.
490 $a(Embrapa Recursos Geneticos e Biotecnologia. Documentos, 45)
520 $aProcedimentos adotados no laboratório de sementes da Embrapa Recursos Genéticos e Biotecnologia para a conservação a médio e longo prazo de sementes de leguminosas forrageiras, para possibilitar que os destinatários de amostras de sementes destas plantas tenham conhecimento da metodologia utilizada, e que os mesmos possam aplicá-la dentro das suas necessidades e possibilidades, ara que este germoplasma seja devidamente manejado, conservado e utilizado, minimizando-se perdas que podem ser irreparáveis.
650 $aforage
650 $agermplasm
650 $agermplasm conservation
650 $aseeds
650 $astorage
650 $aConservação
650 $aGermoplasma
650 $aLegume
650 $aLeguminosa
650 $aLeguminosa Forrageira
650 $aLeguminosae
650 $aRegeneração
650 $aSemente
650 $aTecnologia Apropriada
653 $aAppropriate technology
653 $aArmazenagem
653 $aBAG
653 $aBrasil
653 $aBrasilia
653 $aConservacao de germoplasma
653 $aConservacao de semente
653 $aConservação do germoplasma
653 $aConservation
653 $aFeed crops
653 $aFeed legumes
653 $aForrageira
653 $aGermplas
653 $aLeguminosa forrrageira
653 $aMethods
653 $aMetodologia
653 $aPlanta leguminosa forrageira
653 $aSeed
653 $aSemen preservation
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
13/12/2016 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CAMPOS-JUNIOR, P. H. A.; ALVES, T. J. M.; DIAS, M. T.; ASSUNÇAO, C. M.; MUNK, M.; MATTOS, M. S.; KRAEMER, L. R.; ALMEIDA, B. G.; RUSSO, R. C.; BARCELOS, L.; CAMARGO, L. S. de A.; VIANA, J. H. M. |
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Afiliação: |
PAULO HENRIQUE ALMEIDA CAMPOS-JUNIOR, UFSJ; THALYS JAIR MELO ALVES, UFSJ; MARCO TULIO DIAS, UFSJ; CAROLINA MARINHO ASSUNÇAO, UFJF; MICHELE MUNK, UFJF; MATHEUS SILVÉRIO MATTOS, UFMG; LUCAS ROCHA KRAEMER, UFMG; BRÍGIDA GOMES ALMEIDA, UFMG; REMO CASTRO RUSSO, UFMG; LUCÍOLA BARCELOS, UFMG; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
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Título: |
Ovarian Grafts 10 days after Xenotransplantation: folliculogenesis and recovery of viable oocytes. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Plos One, v. 11, n. 6, e0158109, 2016. |
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DOI: |
10.1371/journal. pone.0158109 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstracts Ovarian xenotransplantation is a promising alternative to preserve fertility of oncologic patients. However, several functional aspects of this procedure remained to be addressed. The aim of this study was evaluate the feasibility of xenotransplantation as a strategy to maintain bovine ovarian grafts and produce oocytes. Adult ovarian cortical pieces were xenotransplanted to the dorsal subcutaneous of female NOD-SCID mice (n = 62). Grafts were recovered ten days after xenotransplantation. Host and graft weights; folliculogenesis progression; blood perfusion, relative gene expression and number of macrophage and neutrophil of xenografts; in vitro developmental competence of graft-derived oocytes were evaluated. Folliculogenesis was supported in the grafts, as indicated by the presence of primordial, primary, secondary, antral, and atretic follicles. The xenografts showed a greater volumetric density of atretic follicles and higher hyperemia and number of host-derived macrophage and neutrophil (P<0.05), when compared to non-grafted fragments. There was a higher blood perfusion under the back skin in the transplantation sites of host animals than in control and non-grafted (P<0.01). BAX and PRDX1 genes were up-regulated, while BCL2, FSHR, IGF1R and IGF2R were down-regulated, when compared to the control (P<0.01). Twenty seven oocytes were successfully harvested from grafts, and some of these oocytes were able to give rise to blastocysts after in vitro fertilization. However, cleavage and blastocyst rates of xenograft derived oocytes were lower than in control (P<0.01). Despite showing some functional modifications, the ovarian xenografts were able to support folliculogenesis and produce functional oocytes. MenosAbstracts Ovarian xenotransplantation is a promising alternative to preserve fertility of oncologic patients. However, several functional aspects of this procedure remained to be addressed. The aim of this study was evaluate the feasibility of xenotransplantation as a strategy to maintain bovine ovarian grafts and produce oocytes. Adult ovarian cortical pieces were xenotransplanted to the dorsal subcutaneous of female NOD-SCID mice (n = 62). Grafts were recovered ten days after xenotransplantation. Host and graft weights; folliculogenesis progression; blood perfusion, relative gene expression and number of macrophage and neutrophil of xenografts; in vitro developmental competence of graft-derived oocytes were evaluated. Folliculogenesis was supported in the grafts, as indicated by the presence of primordial, primary, secondary, antral, and atretic follicles. The xenografts showed a greater volumetric density of atretic follicles and higher hyperemia and number of host-derived macrophage and neutrophil (P<0.05), when compared to non-grafted fragments. There was a higher blood perfusion under the back skin in the transplantation sites of host animals than in control and non-grafted (P<0.01). BAX and PRDX1 genes were up-regulated, while BCL2, FSHR, IGF1R and IGF2R were down-regulated, when compared to the control (P<0.01). Twenty seven oocytes were successfully harvested from grafts, and some of these oocytes were able to give rise to blastocysts after in vitro fertilization. H... Mostrar Tudo |
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Palavras-Chave: |
Bovine ovarian grafts; Ovarian xenografts. |
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Thesaurus NAL: |
oocytes. |
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Categoria do assunto: |
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L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154925/1/Cnpgl-2016-PlosOne-Ovarian.PDF
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Marc: |
LEADER 02628naa a2200301 a 4500
001 2062986
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1371/journal. pone.0158109$2DOI
100 1 $aCAMPOS-JUNIOR, P. H. A.
245 $aOvarian Grafts 10 days after Xenotransplantation$bfolliculogenesis and recovery of viable oocytes.$h[electronic resource]
260 $c2016
520 $aAbstracts Ovarian xenotransplantation is a promising alternative to preserve fertility of oncologic patients. However, several functional aspects of this procedure remained to be addressed. The aim of this study was evaluate the feasibility of xenotransplantation as a strategy to maintain bovine ovarian grafts and produce oocytes. Adult ovarian cortical pieces were xenotransplanted to the dorsal subcutaneous of female NOD-SCID mice (n = 62). Grafts were recovered ten days after xenotransplantation. Host and graft weights; folliculogenesis progression; blood perfusion, relative gene expression and number of macrophage and neutrophil of xenografts; in vitro developmental competence of graft-derived oocytes were evaluated. Folliculogenesis was supported in the grafts, as indicated by the presence of primordial, primary, secondary, antral, and atretic follicles. The xenografts showed a greater volumetric density of atretic follicles and higher hyperemia and number of host-derived macrophage and neutrophil (P<0.05), when compared to non-grafted fragments. There was a higher blood perfusion under the back skin in the transplantation sites of host animals than in control and non-grafted (P<0.01). BAX and PRDX1 genes were up-regulated, while BCL2, FSHR, IGF1R and IGF2R were down-regulated, when compared to the control (P<0.01). Twenty seven oocytes were successfully harvested from grafts, and some of these oocytes were able to give rise to blastocysts after in vitro fertilization. However, cleavage and blastocyst rates of xenograft derived oocytes were lower than in control (P<0.01). Despite showing some functional modifications, the ovarian xenografts were able to support folliculogenesis and produce functional oocytes.
650 $aoocytes
653 $aBovine ovarian grafts
653 $aOvarian xenografts
700 1 $aALVES, T. J. M.
700 1 $aDIAS, M. T.
700 1 $aASSUNÇAO, C. M.
700 1 $aMUNK, M.
700 1 $aMATTOS, M. S.
700 1 $aKRAEMER, L. R.
700 1 $aALMEIDA, B. G.
700 1 $aRUSSO, R. C.
700 1 $aBARCELOS, L.
700 1 $aCAMARGO, L. S. de A.
700 1 $aVIANA, J. H. M.
773 $tPlos One$gv. 11, n. 6, e0158109, 2016.
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Embrapa Gado de Leite (CNPGL) |
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