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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
07/07/2014 |
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Data da última atualização: |
16/01/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VIDAL, Á. M.; COSTA, M. A. P. de C.; SOUZA, A. da S.; ALMEIDA, W. A. B. de; SOUZA, F. V. D. |
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Afiliação: |
ÁDILA MELO VIDAL, UFRB; MARIA ANGÉLICA PEREIRA DE CARVALHO COSTA, UFRB; ANTONIO DA SILVA SOUZA, CNPMF; WELITON ANTONIO BASTOS DE ALMEIDA, FAMAM; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
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Título: |
In vitro regeneration and morphogenesis of somatic embryos of cassava. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Revista Ciência Agronômica, v. 45, n. 3, p. 558-565, jul-set, 2014. |
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ISSN: |
1806-6690 |
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Idioma: |
Inglês |
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Conteúdo: |
In this study, the somatic embryogenesis and regeneration of Cigana Preta plants from shoot apices and immature leaves taken from plants cultivated in vitro were examined. To embryo induction the explants were cultivated in Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 4-amino-3,5,6-trichloropicolinic acid (Picloram) at concentrations of 8.0 and 12 mg L-1. To development of embryos two media cultures with different concentration of BAP (D1 or D2) were evaluated. Embryos in the cotyledonary stage were incubated in germination medium containing MS salts and vitamins, 2.0 ?M copper sulfate, 2.4 g L-1 of Phytagel® and 1.77 ?M BAP. The highest frequency of calluses and the greatest number of embryos per explant were obtained using the auxin picloram at a concentration of 8.0 mg L-1. The plants regenerated in the picloram treatment exhibited normal development and were transferred to a multiplication medium after a minimum of four weeks. The histological sections of the malformed embryos from foliar explants cultured in the presence of 2,4-D demonstrated that the origin of the cotyledonary structures was independent of the formation of the shoot apical meristem, which was not formed in the embryos, and the majority of the embryos were classified as cornet-shaped. This study demonstrates that in cassava, the use of different auxins provides different conditions for the formation of somatic embryos, and the low rate of conversion into plants results from abnormalities in the embryos. MenosIn this study, the somatic embryogenesis and regeneration of Cigana Preta plants from shoot apices and immature leaves taken from plants cultivated in vitro were examined. To embryo induction the explants were cultivated in Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 4-amino-3,5,6-trichloropicolinic acid (Picloram) at concentrations of 8.0 and 12 mg L-1. To development of embryos two media cultures with different concentration of BAP (D1 or D2) were evaluated. Embryos in the cotyledonary stage were incubated in germination medium containing MS salts and vitamins, 2.0 ?M copper sulfate, 2.4 g L-1 of Phytagel® and 1.77 ?M BAP. The highest frequency of calluses and the greatest number of embryos per explant were obtained using the auxin picloram at a concentration of 8.0 mg L-1. The plants regenerated in the picloram treatment exhibited normal development and were transferred to a multiplication medium after a minimum of four weeks. The histological sections of the malformed embryos from foliar explants cultured in the presence of 2,4-D demonstrated that the origin of the cotyledonary structures was independent of the formation of the shoot apical meristem, which was not formed in the embryos, and the majority of the embryos were classified as cornet-shaped. This study demonstrates that in cassava, the use of different auxins provides different conditions for the formation of somatic embryos, and the low rate of conversion into pl... Mostrar Tudo |
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Palavras-Chave: |
Cigana Preta. |
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Thesagro: |
Embriogênese; Mandioca. |
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Thesaurus Nal: |
Cassava; Plant growth substances; Somatic embryogenesis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02261naa a2200253 a 4500
001 1989891
005 2015-01-16
008 2014 bl uuuu u00u1 u #d
022 $a1806-6690
100 1 $aVIDAL, Á. M.
245 $aIn vitro regeneration and morphogenesis of somatic embryos of cassava.
260 $c2014
520 $aIn this study, the somatic embryogenesis and regeneration of Cigana Preta plants from shoot apices and immature leaves taken from plants cultivated in vitro were examined. To embryo induction the explants were cultivated in Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 4-amino-3,5,6-trichloropicolinic acid (Picloram) at concentrations of 8.0 and 12 mg L-1. To development of embryos two media cultures with different concentration of BAP (D1 or D2) were evaluated. Embryos in the cotyledonary stage were incubated in germination medium containing MS salts and vitamins, 2.0 ?M copper sulfate, 2.4 g L-1 of Phytagel® and 1.77 ?M BAP. The highest frequency of calluses and the greatest number of embryos per explant were obtained using the auxin picloram at a concentration of 8.0 mg L-1. The plants regenerated in the picloram treatment exhibited normal development and were transferred to a multiplication medium after a minimum of four weeks. The histological sections of the malformed embryos from foliar explants cultured in the presence of 2,4-D demonstrated that the origin of the cotyledonary structures was independent of the formation of the shoot apical meristem, which was not formed in the embryos, and the majority of the embryos were classified as cornet-shaped. This study demonstrates that in cassava, the use of different auxins provides different conditions for the formation of somatic embryos, and the low rate of conversion into plants results from abnormalities in the embryos.
650 $aCassava
650 $aPlant growth substances
650 $aSomatic embryogenesis
650 $aEmbriogênese
650 $aMandioca
653 $aCigana Preta
700 1 $aCOSTA, M. A. P. de C.
700 1 $aSOUZA, A. da S.
700 1 $aALMEIDA, W. A. B. de
700 1 $aSOUZA, F. V. D.
773 $tRevista Ciência Agronômica$gv. 45, n. 3, p. 558-565, jul-set, 2014.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 4. |  | MENDES, B. M. J.; BOSCARIOL, R. L.; MOURÃO FILHO, F. de A. A.; ALMEIDA, W. A. B. de. Agrobacterium-mediated genetic transformation of 'Hamlin' sweet orange. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 37, n. 7, p. 955-961, jul. 2002 Título em português: Transformação genética de laranja "Hamlin" via agrobacterium.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 5. | | SILVA, R. P. da; COSTA, M. A. P. de C.; SOUZA, A. da S.; ALMEIDA, W. A. B. de. Regeneração de plantas de laranja Pêra via organogênese in vitro. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 40, n. 12, p. 1153-1159, dez. 2005 Título em inglês: Regeneration of 'Pera' sweet orange plants through in vitro organogenesis.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 7. | | COSTA, M. A. P. de C.; ALMEIDA, W. A. B. de; MOURÃO FILHO, F. de A. A.; MENDES, B. M. J.; RODRIGUEZ, A. P. M. Stomatal analysis of citrus somatic hybrids obtained by protoplast fusion. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 39, n. 3, p. 297-300, mar. 2004 Notas Científicas.
Título em português: Análise estomática de híbridos somáticos de citros obtidos por fusão de protoplastos.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 8. | | VIDAL, Á. M.; COSTA, M. A. P. de C.; SOUZA, A. da S.; ALMEIDA, W. A. B. de; SOUZA, F. V. D. In vitro regeneration and morphogenesis of somatic embryos of cassava. Revista Ciência Agronômica, v. 45, n. 3, p. 558-565, jul-set, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 9. | | COSTA, M. A. P. de C.; SOUZA, F. V. D.; LUNA, J. V. U.; CASTELLEN, M. da S.; ALMEIDA, W. A. B. de; SILVA, S. A.; DANTAS, A. C. V. L. Conservação de fruteiras potenciais para o nordeste brasileiro. In: CARVALHO, C. A. L. de; DANTAS, A. C. V. L.; PEREIRA, F. A. de C.; SOARES, A. C. F.; MELO FILHO, J. F. de; OLIVEIRA, G. J. C. de (Org.). Tópicos em ciências agrárias. Cruz das Almas: Universidade Federal do Recôncavo da Bahia, 2009. 296 p. il. p. 3-13| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 10. | | LEMOS, E. E. P. de; COSTA, M. A. P. de C.; ALOUFA, M. A. I.; LEDO, A. da S.; ALMEIDA, W. A. B. de; DANTAS, A. C. V. L.; SILVA, S. A.; SOUZA, F. V. D. Micropropagação. In: SILVA JUNIOR, J. F. da; LEDO, A. da S. (Ed). A cultura da mangaba. Aracaju: Embrapa Tabuleiros Costeiros, 2006. p. 125-133| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 10 | |
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