Data/hora: 20/06/2024 01:53:35

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 15/07/2002
Data da última atualização: | 08/11/2018
Autoria: | MENDES, B. M. J.; BOSCARIOL, R. L.; MOURÃO FILHO, F. de A. A.; ALMEIDA, W. A. B. de.
Afiliação: | BEATRIZ MADALENA JANUZZI MENDES, Universidade de São Paulo - USP/Centro de Energia Nuclear na Agricultura - CENA; RAQUEL LUCIANA BOSCARIOL, Universidade de São Paulo - USP/Centro de Energia Nuclear na Agricultura - CENA; WELITON ANTONIO BASTOS DE ALMEIDA, Escola Superior de Agricultura Luiz de Queiroz - Esalq/Departamento de Produção Vegetal.
Título: | Agrobacterium-mediated genetic transformation of 'Hamlin' sweet orange.
Ano de publicação: | 2002
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, DF, v. 37, n. 7, p. 955-961, jul. 2002
Idioma: | Inglês
Notas: | Título em português: Transformação genética de laranja "Hamlin" via agrobacterium.
Conteúdo: | The development and optimization of efficient transformation protocols is essential in new citrus breeding programs, not only for rootstock, but also for scion improvement. Transgenic 'Hamlin' sweet orange (Citrus sinensis (L.) Osbeck) plants were obtained by Agrobacterium tumefaciens-mediated transformation of epicotyl segments collected from seedlings germinated in vitro. Factors influencing genetic transformation efficiency were evaluated including seedling incubation conditions, time of inoculation with Agrobacterium and co-culture conditions. Epicotyl segments were adequate explants for transformation, regenerating plants by direct organogenesis. Higher percentage of transformation was obtained with explants collected from seedlings germinated in darkness, transferred to 16 hours photoperiod for 2-3 weeks, and inoculated with Agrobacterium for 15-45 min. The best co-culture condition was the incubation of the explants in darkness, for three days in culture medium supplemented with 100 mM of acetosyringone. Genetic transformation was confirmed by performing ß-glucoronidase (GUS) assays and, subsequently, by PCR amplification for the nptII and GUS genes.
Palavras-Chave: | epicótilo; métodos de melhoramento; plântulas; transgênicos; transgenics.
Thesagro: | Citrus Sinensis.
Thesaurus Nal: | breeding methods; epicotyls; seedlings.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/22582/1/pab1158.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (22582-1) - UPE - AP - PP - 630.72081 - P474 -  - DIS