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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
28/03/2013 |
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Data da última atualização: |
12/07/2016 |
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Autoria: |
ALMEIDA, L. M. de; ALMEIDA, M. Z. P. R. B. de; MENDONCA, C. L. de; MAMIZUKA, E. M. |
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Título: |
Novel sequence types (STs) of Staphylococcus aureus isolates causing clinical and subclinical mastitis in flocks of sheep in the northeast of Brazil. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Journal of Dairy Research, v. 78, n. 3, p. 373-378, Aug. 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
Staphylococcus aureus is one of the most important infectious mastitis causative agents in small ruminants. In order to know the distribution of Staph. aureus strains associated with infectious mastitis in flocks of sheep in the northeast of Brazil and establish whether these clones are related to the strains distributed internationally, this study analysed the genetic diversity of Staph. aureus isolates from cases of clinical and subclinical mastitis in ewes by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). In this research, 135 ewes with mastitis from 31 sheep flocks distributed in 15 districts were examined. Staph. aureus was isolated from sheep milk in 9 (29%) out of 31 herds located in 47% of the districts surveyed. MLST analysis allowed the identification of four STs (ST750, ST1728, ST1729 and ST1730). The last three with their respective novel alleles (g/p-220; pta-182 and yqil-180) were recently reported in the Staph. aureus MLST database (http://www.mlst.net). Each novel allele showed only a nucleotide different from those already described. The occurrence of CC133 (ST750 and ST1729) in this study is in agreement with other reports that only a few clones of Staph. aureus seem to be responsible for most cases of mastitis in dairy farms and that some of these clones may have broad geographic distribution. However, the prevalence of CC5 (ST1728 and ST1730)-an important group related to cases of colonization or infection in humans-differs from previous studies by its widespread occurrence and may suggest human contamination followed by selective pressures of the allelic diversifications presented for these STs. MenosStaphylococcus aureus is one of the most important infectious mastitis causative agents in small ruminants. In order to know the distribution of Staph. aureus strains associated with infectious mastitis in flocks of sheep in the northeast of Brazil and establish whether these clones are related to the strains distributed internationally, this study analysed the genetic diversity of Staph. aureus isolates from cases of clinical and subclinical mastitis in ewes by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). In this research, 135 ewes with mastitis from 31 sheep flocks distributed in 15 districts were examined. Staph. aureus was isolated from sheep milk in 9 (29%) out of 31 herds located in 47% of the districts surveyed. MLST analysis allowed the identification of four STs (ST750, ST1728, ST1729 and ST1730). The last three with their respective novel alleles (g/p-220; pta-182 and yqil-180) were recently reported in the Staph. aureus MLST database (http://www.mlst.net). Each novel allele showed only a nucleotide different from those already described. The occurrence of CC133 (ST750 and ST1729) in this study is in agreement with other reports that only a few clones of Staph. aureus seem to be responsible for most cases of mastitis in dairy farms and that some of these clones may have broad geographic distribution. However, the prevalence of CC5 (ST1728 and ST1730)-an important group related to cases of colonization or infection in humans-differs... Mostrar Tudo |
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Thesagro: |
Mamite; Microbiologia; Ovino; Staphylococcus aureus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02360naa a2200205 a 4500
001 1954493
005 2016-07-12
008 2011 bl uuuu u00u1 u #d
100 1 $aALMEIDA, L. M. de
245 $aNovel sequence types (STs) of Staphylococcus aureus isolates causing clinical and subclinical mastitis in flocks of sheep in the northeast of Brazil.$h[electronic resource]
260 $c2011
520 $aStaphylococcus aureus is one of the most important infectious mastitis causative agents in small ruminants. In order to know the distribution of Staph. aureus strains associated with infectious mastitis in flocks of sheep in the northeast of Brazil and establish whether these clones are related to the strains distributed internationally, this study analysed the genetic diversity of Staph. aureus isolates from cases of clinical and subclinical mastitis in ewes by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). In this research, 135 ewes with mastitis from 31 sheep flocks distributed in 15 districts were examined. Staph. aureus was isolated from sheep milk in 9 (29%) out of 31 herds located in 47% of the districts surveyed. MLST analysis allowed the identification of four STs (ST750, ST1728, ST1729 and ST1730). The last three with their respective novel alleles (g/p-220; pta-182 and yqil-180) were recently reported in the Staph. aureus MLST database (http://www.mlst.net). Each novel allele showed only a nucleotide different from those already described. The occurrence of CC133 (ST750 and ST1729) in this study is in agreement with other reports that only a few clones of Staph. aureus seem to be responsible for most cases of mastitis in dairy farms and that some of these clones may have broad geographic distribution. However, the prevalence of CC5 (ST1728 and ST1730)-an important group related to cases of colonization or infection in humans-differs from previous studies by its widespread occurrence and may suggest human contamination followed by selective pressures of the allelic diversifications presented for these STs.
650 $aMamite
650 $aMicrobiologia
650 $aOvino
650 $aStaphylococcus aureus
700 1 $aALMEIDA, M. Z. P. R. B. de
700 1 $aMENDONCA, C. L. de
700 1 $aMAMIZUKA, E. M.
773 $tJournal of Dairy Research$gv. 78, n. 3, p. 373-378, Aug. 2011.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Tipo/Formato |
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Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
14/01/2020 |
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Data da última atualização: |
14/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SABINO, A. R.; TAVARES, S. S.; RIFFEL, A.; LI, J. V.; OLIVEIRA, D. J. A.; FERES, C. I. M. A.; HENRIQUE, L.; OLIVEIRA, J. S.; CORREIA, G. D. S.; SABINO, A. R.; NASCIMENTO, T. G.; HAWKES, G.; SANTANA, A. E. G.; HOLMES, E.; BENDO, E. S. |
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Afiliação: |
ADILSON R. SABINO; SHEILA S. TAVARES; ALESSANDRO RIFFEL, CPATC; JIA V. LI; DEMETRIOS J. A. OLIVEIRA; CHRYSTIAN I. M. A. FERES; JAIM S. OLIVEIRA; GONCALO D. S. CORREIA; ANDERSON R. SABINO; TICIANO G. NASCIMENTO; GEOFREY HAWKES; ANTONIO E. G. SANTANA; ELAINE HOLMES; EDSON S. BENTO. |
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Título: |
1H NMR metabolomic approach reveals chlorogenic acid as a response ofsugarcane induced by exposure toDiatraea saccharalis. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Industrial Crops & Products, V. 140, 111651, 2019. |
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Idioma: |
Português |
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Conteúdo: |
Sugarcane(Saccharum officinarum)has been considered one of the most efficient energy crops, but its productionyield is sensitive to outbreaks of pest insects, especially the sugarcane borerDiatraea saccharalis. Geneticbreeding programs and biotechnology projects have been developed to decode the defense mechanisms of su-garcane against herbivorous insect attacks, and the develop plague-resistant plants. We performed metabolicprofile analysis of the SP791011 sugarcane variety?s response toDiatraea saccharalisherbivory, using NuclearMagnetic Resonance (NMR) spectroscopy of organic leaf extracts. The leaf response of SP791011 toD. saccharalisresulted in depletion of choline, alanine, sucrose, glutamate, trigonelline, and isomers (E)-aconitate, (Z)-aco-nitate, and higher expression of chlorogenic acid and other caffeic acid conjugates in sugarcane leaves. Theincrease in chlorogenic acid suggests the shikimic acid pathway was induced byD. saccharalisherbivory, in-creasing the biosynthesis of phenylpropanoids such as chlorogenic acid in the sugarcane leaves. In addition tothe herbivory test, we performed an in vivo biological assay by adding chlorogenic acid to an artificial diet toD.saccharaliscaterpillars. This assay demonstrated a decrease in the development time of the pupae compared withpupae from caterpillars raised under normal diet. However, deformations in moth wings fed with chlorogenicacid were observed for three concentrations tested (0.05 mg/mL, 0.5 mg/mL and 5 mg/mL) during the in vivobioassay. Chlorogenic acid may be considered a natural biopesticide and its production could be induced todevelop more resistant sugarcane varieties againstD. saccharalis. MenosSugarcane(Saccharum officinarum)has been considered one of the most efficient energy crops, but its productionyield is sensitive to outbreaks of pest insects, especially the sugarcane borerDiatraea saccharalis. Geneticbreeding programs and biotechnology projects have been developed to decode the defense mechanisms of su-garcane against herbivorous insect attacks, and the develop plague-resistant plants. We performed metabolicprofile analysis of the SP791011 sugarcane variety?s response toDiatraea saccharalisherbivory, using NuclearMagnetic Resonance (NMR) spectroscopy of organic leaf extracts. The leaf response of SP791011 toD. saccharalisresulted in depletion of choline, alanine, sucrose, glutamate, trigonelline, and isomers (E)-aconitate, (Z)-aco-nitate, and higher expression of chlorogenic acid and other caffeic acid conjugates in sugarcane leaves. Theincrease in chlorogenic acid suggests the shikimic acid pathway was induced byD. saccharalisherbivory, in-creasing the biosynthesis of phenylpropanoids such as chlorogenic acid in the sugarcane leaves. In addition tothe herbivory test, we performed an in vivo biological assay by adding chlorogenic acid to an artificial diet toD.saccharaliscaterpillars. This assay demonstrated a decrease in the development time of the pupae compared withpupae from caterpillars raised under normal diet. However, deformations in moth wings fed with chlorogenicacid were observed for three concentrations tested (0.05 mg/mL, 0.5 mg/mL and 5 mg/mL)... Mostrar Tudo |
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Thesagro: |
Cana de Açúcar; Inseto; Inseto Para Controle Biológico. |
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Thesaurus NAL: |
Sugarcane. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208742/1/2019-h1nmr.pdf
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Marc: |
LEADER 02668naa a2200337 a 4500
001 2118719
005 2020-01-14
008 2019 bl uuuu u00u1 u #d
100 1 $aSABINO, A. R.
245 $a1H NMR metabolomic approach reveals chlorogenic acid as a response ofsugarcane induced by exposure toDiatraea saccharalis.$h[electronic resource]
260 $c2019
520 $aSugarcane(Saccharum officinarum)has been considered one of the most efficient energy crops, but its productionyield is sensitive to outbreaks of pest insects, especially the sugarcane borerDiatraea saccharalis. Geneticbreeding programs and biotechnology projects have been developed to decode the defense mechanisms of su-garcane against herbivorous insect attacks, and the develop plague-resistant plants. We performed metabolicprofile analysis of the SP791011 sugarcane variety?s response toDiatraea saccharalisherbivory, using NuclearMagnetic Resonance (NMR) spectroscopy of organic leaf extracts. The leaf response of SP791011 toD. saccharalisresulted in depletion of choline, alanine, sucrose, glutamate, trigonelline, and isomers (E)-aconitate, (Z)-aco-nitate, and higher expression of chlorogenic acid and other caffeic acid conjugates in sugarcane leaves. Theincrease in chlorogenic acid suggests the shikimic acid pathway was induced byD. saccharalisherbivory, in-creasing the biosynthesis of phenylpropanoids such as chlorogenic acid in the sugarcane leaves. In addition tothe herbivory test, we performed an in vivo biological assay by adding chlorogenic acid to an artificial diet toD.saccharaliscaterpillars. This assay demonstrated a decrease in the development time of the pupae compared withpupae from caterpillars raised under normal diet. However, deformations in moth wings fed with chlorogenicacid were observed for three concentrations tested (0.05 mg/mL, 0.5 mg/mL and 5 mg/mL) during the in vivobioassay. Chlorogenic acid may be considered a natural biopesticide and its production could be induced todevelop more resistant sugarcane varieties againstD. saccharalis.
650 $aSugarcane
650 $aCana de Açúcar
650 $aInseto
650 $aInseto Para Controle Biológico
700 1 $aTAVARES, S. S.
700 1 $aRIFFEL, A.
700 1 $aLI, J. V.
700 1 $aOLIVEIRA, D. J. A.
700 1 $aFERES, C. I. M. A.
700 1 $aHENRIQUE, L.
700 1 $aOLIVEIRA, J. S.
700 1 $aCORREIA, G. D. S.
700 1 $aSABINO, A. R.
700 1 $aNASCIMENTO, T. G.
700 1 $aHAWKES, G.
700 1 $aSANTANA, A. E. G.
700 1 $aHOLMES, E.
700 1 $aBENDO, E. S.
773 $tIndustrial Crops & Products, V. 140, 111651, 2019.
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Registro original: |
Embrapa Tabuleiros Costeiros (CPATC) |
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