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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
13/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AMARAL, D. L.; ZANETTE, R. S.; ALMEIDA, C. G.; ALMEIDA, L. B.; OLIVEIRA, L. F.; MARCOMINI, R. F.; NOGUEIRA, B. V.; SANTOS, M. O.; BRANDAO, H. de M.; MARANDUBA, C. M. C.; MUNK, M. |
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Afiliação: |
HUMBERTO DE MELLO BRANDAO, CNPGL. |
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Título: |
In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Biomedical Materials, v. 14, article 035011, 2019. |
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DOI: |
https://doi.org/10.1088/1748-605x/ab0a52 |
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Idioma: |
Inglês |
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Conteúdo: |
Nanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering. In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation. In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC). Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays. Overall, neither pure BT NP or BT NP/alginate 3D scaffold induced cytotoxicity. The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation. Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold. Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements. These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic human MSC differentiation. MenosNanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering. In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation. In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC). Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays. Overall, neither pure BT NP or BT NP/alginate 3D scaffold induced cytotoxicity. The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation. Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold. Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements. These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic... Mostrar Tudo |
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Palavras-Chave: |
3D scaffolds; Human stem cells; Nanomaterial; Nanotopography; Osteoinduction. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02473naa a2200313 a 4500
001 2118648
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1088/1748-605x/ab0a52$2DOI
100 1 $aAMARAL, D. L.
245 $aIn vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation.$h[electronic resource]
260 $c2019
520 $aNanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering. In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation. In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC). Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays. Overall, neither pure BT NP or BT NP/alginate 3D scaffold induced cytotoxicity. The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation. Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold. Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements. These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic human MSC differentiation.
653 $a3D scaffolds
653 $aHuman stem cells
653 $aNanomaterial
653 $aNanotopography
653 $aOsteoinduction
700 1 $aZANETTE, R. S.
700 1 $aALMEIDA, C. G.
700 1 $aALMEIDA, L. B.
700 1 $aOLIVEIRA, L. F.
700 1 $aMARCOMINI, R. F.
700 1 $aNOGUEIRA, B. V.
700 1 $aSANTOS, M. O.
700 1 $aBRANDAO, H. de M.
700 1 $aMARANDUBA, C. M. C.
700 1 $aMUNK, M.
773 $tBiomedical Materials$gv. 14, article 035011, 2019.
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Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 24 | |
| 21. |  | GREGO, C. R.; RODRIGUES, C. A. G.; NOGUEIRA, S. F.; GIMENES, F. M. A.; OLIVEIRA, A. DE; ALMEIDA, C. G. F. DE; FURTADO, A. L. dos S.; DEMARCHI, J. J. A. DE A. Variabilidade espacial do solo e da biomassa epígea de pastagem, identificada por meio de geostatística. Pesquisa Agropecuária Brasileira, Brasilia, DF, v. 47, n. 9, p. 1404-1412, set. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Territorial; Embrapa Unidades Centrais. |
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| 22. | | AMARAL, D. L.; ZANETTE, R. S.; ALMEIDA, C. G.; ALMEIDA, L. B.; OLIVEIRA, L. F.; MARCOMINI, R. F.; NOGUEIRA, B. V.; SANTOS, M. O.; BRANDAO, H. de M.; MARANDUBA, C. M. C.; MUNK, M. In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation. Biomedical Materials, v. 14, article 035011, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
|  |
| 23. | | MUNK, M.; ZANETTE, R. de S. S.; CAMARGO, L. S. de A.; SOUZA, N. L. G. D. de; ALMEIDA, C. G. de; GERN, J. C.; GUIMARAES, A. S.; LADEIRA, L. O.; OLIVEIRA, L. F. C. de; BRANDAO, H. de M. Using carbon nanotubes to deliver genes to hard-to-transfect mammalian primary fibroblast cells. Biomedical Physics & Engineering Express, v. 3, n. 4, 045002, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 24. | | SILVA, N. P. da; PEREIRA, E. do C. R. L.; DUARTE, L. M.; FREITAS, J. C. de O.; ALMEIDA, C. G. de; SILVA, T. P. da; MELO, R. C. N.; APOLONIO, A. C. M.; OLIVEIRA, M. A. L. de; BRANDAO, H. de M.; PITTELLA, F.; FABRI, R. L.; TAVARES, G. D.; PINTO, P. de F. Improved anti-Cutibacterium acnes activity of tea tree oil-loaded chitosan-poly(ε-caprolactone) core-shell nanocapsules. Colloids and Surfaces B: Biointerfaces, v. 196, 111371, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 24 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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