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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
10/12/2014 |
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Data da última atualização: |
10/12/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AMABILE, R. F.; FALEIRO, F. G.; CAPETTINI, F.; RIBEIRO JUNIOR, W. Q.; PEIXOTO, J. R.; ALMEIDA, B. C. de. |
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Afiliação: |
RENATO FERNANDO AMABILE, CPAC; FABIO GELAPE FALEIRO, CPAC; WALTER QUADROS RIBEIRO JUNIOR, CPAC. |
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Título: |
Genetic variability of elite barley genotypes for Brazilian Savanna irrigated systems based on RAPD. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Bioscience Journal, Uberlândia, v. 30, n. 4, July/Aug. 2014. |
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Páginas: |
p. 1118-1126 |
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Idioma: |
Português |
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Palavras-Chave: |
Diversidade genética; RAPD. |
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Thesagro: |
Cevada; Hordeum vulgare. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 00676naa a2200229 a 4500
001 2002097
005 2014-12-10
008 2014 bl --- 0-- u #d
100 1 $aAMABILE, R. F.
245 $aGenetic variability of elite barley genotypes for Brazilian Savanna irrigated systems based on RAPD.
260 $c2014
300 $ap. 1118-1126
650 $aCevada
650 $aHordeum vulgare
653 $aDiversidade genética
653 $aRAPD
700 1 $aFALEIRO, F. G.
700 1 $aCAPETTINI, F.
700 1 $aRIBEIRO JUNIOR, W. Q.
700 1 $aPEIXOTO, J. R.
700 1 $aALMEIDA, B. C. de
773 $tBioscience Journal, Uberlândia$gv. 30, n. 4, July/Aug. 2014.
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Embrapa Cerrados (CPAC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
11/09/1997 |
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Data da última atualização: |
11/09/1997 |
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Autoria: |
SILVA, R. A. M. S.; RAMIREZ, L.; DAVILA, A. M. R.; VICTORIO, A. M.; PEREIRA, M. E. B. |
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Afiliação: |
EMBRAPA. Centro de Pesquisa Agropecuaria do Pantanal (Corumba, MS); UFMS. Centro Universitario de Corumba. Departamento de Ciencias do Ambiente (Corumba, MS). |
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Título: |
An investigation of in vitro phagocytosis of Trypanosoma evansi by rat peritoneal macrophages: increase mediated by parasite-specific antibodies. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Memorias do Instituto Oswaldo Cruz, Rio de Janeiro, v.91, p.105, 1996. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Trypanosoma evansi causes and usually fatal disease in rats, mice, horses and dogs. Phagocytosis and the intracellular killing of pathogens in probaly one of the most important defense system available to an animal. Macrophages (Mo) are central cells in the action and regulation of the immune network. This is in view of their function as phagocytic and antigen-presenting cells, are their release of mediators, expression of surface receptors, and interaction with T and B cells. This experiment was developed to evaluate the influence of parasitic-specific antibody in the phagocytosis by peritoneal Mo of rats. Peritoneal Mo, 1,45 x 10 6 were isolated from Wistar rats pretreated 3 days before single intraperitoneal dose of tioglicolate. The Mo were then precultivated for 24 h. on coverslips in culture flasks. Trypanosomes, 8,7 x 10 6(final concentration) were added to the adherent macrophages and were cocultivated at 37 oC for 5, 15, 30, 45 min. in EAGLE medium (Instituto Adolfo Lutz, SP). The trypanosome/ Mo proportion was 6:1. The phagocytosis test was performed in two groups: group A (Mo treated with normal serum) and B (Mo treated with 200 ul of serum from horse naturally infected by T.evansi). The parasites were not observed attached or phagocytosed by Mo in group A. In group B trypanosomes attached to 51,26% of the Mos within 5 min, 17,20% within 15 min, 46,15% within 30 min, 10,65% within 45 min. The phagocytosis process was observed in 96,63% of the Mos at 5 min, 83,87% at 15 min, 100% at 30 min, and 94,26% at 45 min. We conclude that the absence of phagocytosis in the group A could be explained normal macrophages have some difficulty to uptake the parasites; although some of parasites attach to the Mo, they are not internalized as observed in experiments with T.brucei. The espace from phagocytosis is even improved because of short cell contacts due to permanent movement of the cells concerned. However, in the presence of parasite-specific antibodies (group B), internalization and degradationof the trypanosomes took place. So, according to Shakibaei & Frevert, in the absence of antibodies, bloodstream trypanosomes are protected against phagocytosis by the surface coat. In immune animals the parasites are removed from the bloodsteam by phagocytic cells. The opsonization with specific antibodies or proteolytic removal of the coat result in rapid uptake of the parasites by Mos. MenosTrypanosoma evansi causes and usually fatal disease in rats, mice, horses and dogs. Phagocytosis and the intracellular killing of pathogens in probaly one of the most important defense system available to an animal. Macrophages (Mo) are central cells in the action and regulation of the immune network. This is in view of their function as phagocytic and antigen-presenting cells, are their release of mediators, expression of surface receptors, and interaction with T and B cells. This experiment was developed to evaluate the influence of parasitic-specific antibody in the phagocytosis by peritoneal Mo of rats. Peritoneal Mo, 1,45 x 10 6 were isolated from Wistar rats pretreated 3 days before single intraperitoneal dose of tioglicolate. The Mo were then precultivated for 24 h. on coverslips in culture flasks. Trypanosomes, 8,7 x 10 6(final concentration) were added to the adherent macrophages and were cocultivated at 37 oC for 5, 15, 30, 45 min. in EAGLE medium (Instituto Adolfo Lutz, SP). The trypanosome/ Mo proportion was 6:1. The phagocytosis test was performed in two groups: group A (Mo treated with normal serum) and B (Mo treated with 200 ul of serum from horse naturally infected by T.evansi). The parasites were not observed attached or phagocytosed by Mo in group A. In group B trypanosomes attached to 51,26% of the Mos within 5 min, 17,20% within 15 min, 46,15% within 30 min, 10,65% within 45 min. The phagocytosis process was observed in 96,63% of the Mos at 5 min, 83,87%... Mostrar Tudo |
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Thesaurus NAL: |
Trypanosoma evansi. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03040naa a2200181 a 4500
001 1791630
005 1997-09-11
008 1996 bl uuuu u00u1 u #d
100 1 $aSILVA, R. A. M. S.
245 $aAn investigation of in vitro phagocytosis of Trypanosoma evansi by rat peritoneal macrophages$bincrease mediated by parasite-specific antibodies.
260 $c1996
520 $aTrypanosoma evansi causes and usually fatal disease in rats, mice, horses and dogs. Phagocytosis and the intracellular killing of pathogens in probaly one of the most important defense system available to an animal. Macrophages (Mo) are central cells in the action and regulation of the immune network. This is in view of their function as phagocytic and antigen-presenting cells, are their release of mediators, expression of surface receptors, and interaction with T and B cells. This experiment was developed to evaluate the influence of parasitic-specific antibody in the phagocytosis by peritoneal Mo of rats. Peritoneal Mo, 1,45 x 10 6 were isolated from Wistar rats pretreated 3 days before single intraperitoneal dose of tioglicolate. The Mo were then precultivated for 24 h. on coverslips in culture flasks. Trypanosomes, 8,7 x 10 6(final concentration) were added to the adherent macrophages and were cocultivated at 37 oC for 5, 15, 30, 45 min. in EAGLE medium (Instituto Adolfo Lutz, SP). The trypanosome/ Mo proportion was 6:1. The phagocytosis test was performed in two groups: group A (Mo treated with normal serum) and B (Mo treated with 200 ul of serum from horse naturally infected by T.evansi). The parasites were not observed attached or phagocytosed by Mo in group A. In group B trypanosomes attached to 51,26% of the Mos within 5 min, 17,20% within 15 min, 46,15% within 30 min, 10,65% within 45 min. The phagocytosis process was observed in 96,63% of the Mos at 5 min, 83,87% at 15 min, 100% at 30 min, and 94,26% at 45 min. We conclude that the absence of phagocytosis in the group A could be explained normal macrophages have some difficulty to uptake the parasites; although some of parasites attach to the Mo, they are not internalized as observed in experiments with T.brucei. The espace from phagocytosis is even improved because of short cell contacts due to permanent movement of the cells concerned. However, in the presence of parasite-specific antibodies (group B), internalization and degradationof the trypanosomes took place. So, according to Shakibaei & Frevert, in the absence of antibodies, bloodstream trypanosomes are protected against phagocytosis by the surface coat. In immune animals the parasites are removed from the bloodsteam by phagocytic cells. The opsonization with specific antibodies or proteolytic removal of the coat result in rapid uptake of the parasites by Mos.
650 $aTrypanosoma evansi
700 1 $aRAMIREZ, L.
700 1 $aDAVILA, A. M. R.
700 1 $aVICTORIO, A. M.
700 1 $aPEREIRA, M. E. B.
773 $tMemorias do Instituto Oswaldo Cruz, Rio de Janeiro$gv.91, p.105, 1996. Suplemento.
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