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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
24/08/2017 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. |
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Afiliação: |
Helena Fabiana Reis de Almeida Saraiva, Universidade de São Paulo (USA) - Pirassununga, SP, Brasil; Ribrio Ivan Tavares Pereira Batista, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brasil; Vivian Angélico Pereira Alfradique, UFF - Niterói, RJ, Brasil; Pedro Henrique Nicolau Pinto, UFF - Niterói, RJ, Brasil; Lilian Santos Ribeiro, UFF - Niterói, RJ, Brasil; CLARA SLADE OLIVEIRA, CNPGL; Joanna Maria Gonçalves de Souza Fabjan, UFF - Niterói, RJ, Brasil; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Zandonadi Brandão, UFF - Niterói, RJ, Brasil. |
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Título: |
L-carnitine supplementation during vitrification did not improve survival and quality rates, but altered CrAT and PRDX1 expression in in vivo-produced ovine embryos. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Animal Reprodroduction, v. 14, n. 3, p. 898, Jul./Sept. 2017. |
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Idioma: |
Inglês |
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Notas: |
Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts. |
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Conteúdo: |
Embryo cryodamage is observed mainly at metabolic and molecular aspects and it impairs post warming quality and survival rates. This study aimed to evaluate the effect of L-carnitine (LC) supplementation during either vitrification or post warming solutions on the 6-7th day of in vivo-produced ovine embryos. LC (3.72 mM) was added to vitrification (Experiment 1; C1: control; LC1: supplemented embryos) or warming solutions (Experiment 2; C2; LC2). In vitro culture (IVC) of warmed embryos was performed for 72 h at 38,5 °C, 5% CO2 and 5% O2 to evaluate survival rates in both Experiments. In Experiment 1, reactive oxygen species (ROS) levels were measured by CellROX Green staining, total cell number (TCN) by Hoechst 33342, number of apoptotic cells by caspase-3 immunofluorescence staining protocol, apoptotic index evaluation in both groups. Gene expression analysis of carnitine palmitoyltransferase 1 and 2 (CPT1 and CPT2), carnitine O-acyltransferase (CrAT) and peroxiredoxin 1 (PRDX1), were performed by RT-qPCR (ACTB as endogenous control) in Experiments 1 and 2 and results were compared to fresh embryos (FE). Averages of survival rates were compared by the Chi-Square test. Means of TCN, apoptotic cells, apoptotic index and fluorescence intensity were compared by Student's t-test, at 5% significance level. Survival rates were similar between groups (p> 0.05) in Experiments 1 (68.7%, C1 vs 81.8%, LC1) and 2 (48.5%, C2 vs 64.7%, LC2). In Experiment 1, ROS levels at 24 h of IVC (85.83 ± 68.37 x 1010, C1 vs 89.04 ± 84.48 x 1010, LC1), total cell number at 24 h (89 ± 22, C1 vs 82.2 ± 28, LC1) and 72 h (86 ± 19.9, C1 vs 68.5 ± 25.26, LC1), apoptotic cells (3.75 ± 1.48, C1 vs 4.50 ± 4.72, LC1) and apoptotic index (4.37 ± 1.45, C1 vs 5.23 ± 4.72, LC1) at 72 h of IVC did not differ (p> 0.05) between C1 and LC1. Gene expression analysis showed no differences in CPT1 and CPT2 mRNA relative abundance in embryos of both experiments compared to FE, however, CrAT was downregulated (p< 0.05) in C1 and PRDX1 was downregulated (p< 0.05) in both C1 and LC1, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p< 0.05) in C2 and CrAT was downregulated (p< 0.05) in LC2, in relation to FE. In conclusion, although the short-term LC supplementation at 3.72 mM during cryopreservation did not improve post-warming survival and morphological parameters of the evaluated embryos, it was able to modulate expression of genes related to energy homeostasis (CrAT) and oxidative stress (PRDX1), proving to be beneficial, in both forms of supplementation, to in vivo-produced ovine embryos.". MenosEmbryo cryodamage is observed mainly at metabolic and molecular aspects and it impairs post warming quality and survival rates. This study aimed to evaluate the effect of L-carnitine (LC) supplementation during either vitrification or post warming solutions on the 6-7th day of in vivo-produced ovine embryos. LC (3.72 mM) was added to vitrification (Experiment 1; C1: control; LC1: supplemented embryos) or warming solutions (Experiment 2; C2; LC2). In vitro culture (IVC) of warmed embryos was performed for 72 h at 38,5 °C, 5% CO2 and 5% O2 to evaluate survival rates in both Experiments. In Experiment 1, reactive oxygen species (ROS) levels were measured by CellROX Green staining, total cell number (TCN) by Hoechst 33342, number of apoptotic cells by caspase-3 immunofluorescence staining protocol, apoptotic index evaluation in both groups. Gene expression analysis of carnitine palmitoyltransferase 1 and 2 (CPT1 and CPT2), carnitine O-acyltransferase (CrAT) and peroxiredoxin 1 (PRDX1), were performed by RT-qPCR (ACTB as endogenous control) in Experiments 1 and 2 and results were compared to fresh embryos (FE). Averages of survival rates were compared by the Chi-Square test. Means of TCN, apoptotic cells, apoptotic index and fluorescence intensity were compared by Student's t-test, at 5% significance level. Survival rates were similar between groups (p> 0.05) in Experiments 1 (68.7%, C1 vs 81.8%, LC1) and 2 (48.5%, C2 vs 64.7%, LC2). In Experiment 1, ROS levels at 24 h of IVC (85... Mostrar Tudo |
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Palavras-Chave: |
Animal embryos; Santa Inês; Survival. |
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Thesaurus Nal: |
Carnitine; Cell culture; Cryopreservation; Ewes; Gene expression; In vitro culture; Sheep; Vitrification. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163011/1/CNPC-2017-L-carnitine.pdf
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Marc: |
LEADER 03898nam a2200361 a 4500
001 2074433
005 2019-09-23
008 2017 bl uuuu u00u1 u #d
100 1 $aSARAIVA, H. F. R. de A.
245 $aL-carnitine supplementation during vitrification did not improve survival and quality rates, but altered CrAT and PRDX1 expression in in vivo-produced ovine embryos.$h[electronic resource]
260 $aAnimal Reprodroduction, v. 14, n. 3, p. 898, Jul./Sept. 2017.$c2017
500 $aProceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.
520 $aEmbryo cryodamage is observed mainly at metabolic and molecular aspects and it impairs post warming quality and survival rates. This study aimed to evaluate the effect of L-carnitine (LC) supplementation during either vitrification or post warming solutions on the 6-7th day of in vivo-produced ovine embryos. LC (3.72 mM) was added to vitrification (Experiment 1; C1: control; LC1: supplemented embryos) or warming solutions (Experiment 2; C2; LC2). In vitro culture (IVC) of warmed embryos was performed for 72 h at 38,5 °C, 5% CO2 and 5% O2 to evaluate survival rates in both Experiments. In Experiment 1, reactive oxygen species (ROS) levels were measured by CellROX Green staining, total cell number (TCN) by Hoechst 33342, number of apoptotic cells by caspase-3 immunofluorescence staining protocol, apoptotic index evaluation in both groups. Gene expression analysis of carnitine palmitoyltransferase 1 and 2 (CPT1 and CPT2), carnitine O-acyltransferase (CrAT) and peroxiredoxin 1 (PRDX1), were performed by RT-qPCR (ACTB as endogenous control) in Experiments 1 and 2 and results were compared to fresh embryos (FE). Averages of survival rates were compared by the Chi-Square test. Means of TCN, apoptotic cells, apoptotic index and fluorescence intensity were compared by Student's t-test, at 5% significance level. Survival rates were similar between groups (p> 0.05) in Experiments 1 (68.7%, C1 vs 81.8%, LC1) and 2 (48.5%, C2 vs 64.7%, LC2). In Experiment 1, ROS levels at 24 h of IVC (85.83 ± 68.37 x 1010, C1 vs 89.04 ± 84.48 x 1010, LC1), total cell number at 24 h (89 ± 22, C1 vs 82.2 ± 28, LC1) and 72 h (86 ± 19.9, C1 vs 68.5 ± 25.26, LC1), apoptotic cells (3.75 ± 1.48, C1 vs 4.50 ± 4.72, LC1) and apoptotic index (4.37 ± 1.45, C1 vs 5.23 ± 4.72, LC1) at 72 h of IVC did not differ (p> 0.05) between C1 and LC1. Gene expression analysis showed no differences in CPT1 and CPT2 mRNA relative abundance in embryos of both experiments compared to FE, however, CrAT was downregulated (p< 0.05) in C1 and PRDX1 was downregulated (p< 0.05) in both C1 and LC1, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p< 0.05) in C2 and CrAT was downregulated (p< 0.05) in LC2, in relation to FE. In conclusion, although the short-term LC supplementation at 3.72 mM during cryopreservation did not improve post-warming survival and morphological parameters of the evaluated embryos, it was able to modulate expression of genes related to energy homeostasis (CrAT) and oxidative stress (PRDX1), proving to be beneficial, in both forms of supplementation, to in vivo-produced ovine embryos.".
650 $aCarnitine
650 $aCell culture
650 $aCryopreservation
650 $aEwes
650 $aGene expression
650 $aIn vitro culture
650 $aSheep
650 $aVitrification
653 $aAnimal embryos
653 $aSanta Inês
653 $aSurvival
700 1 $aBATISTA, R. I. T. P.
700 1 $aALFRADIQUE, V. A. P.
700 1 $aPINTO, P. H. N.
700 1 $aRIBEIRO, L. S.
700 1 $aOLIVEIRA, C. S.
700 1 $aSOUZA FABJAN, J. M. G. de
700 1 $aCAMARGO, L. S. de A.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDÃO, F. Z.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
29/04/2023 |
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Data da última atualização: |
07/06/2023 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
PANETTO, J. C. do C.; SILVA, M. V. G. B.; VERNEQUE, R. da S.; MACHADO, M. A.; FERNANDES, A. R.; TEIXEIRA, R. B.; MACHADO, C. H. C.; MARTINS, M. F.; REIS, D. R. de L.; BORGES, C. A. V.; OLIVEIRA, J. C. de; VENTURA, H. T.; PEREIRA, M. A.; MACHADO, M. S.; MOURA, G. M. (ed.). |
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Afiliação: |
JOAO CLAUDIO DO CARMO PANETTO, CNPGL; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; RUI DA SILVA VERNEQUE, CNPGL; MARCO ANTONIO MACHADO, CNPGL; ANDRÉ RABELO FERNANDES, Associação Brasileira dos Criadores de Gir Leiteiro; RAFAEL BASTOS TEIXEIRA, Associação Brasileira dos Criadores de Gir Leiteiro; CARLOS HENRIQUE CAVALLARI MACHADO, Programa Nacional de Melhoramento do Gir Leiteiro; MARTA FONSECA MARTINS, CNPGL; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; CRISTIANO AMANCIO VIEIRA BORGES, CNPGL; JEAN CARLOS DE OLIVEIRA, Programa Nacional de Melhoramento do Gir Leiteiro; HENRIQUE TORRES VENTURA, Associação Brasileira dos Criadores de Zebu; MARIANA ALENCAR PEREIRA, Associação Brasileira dos Criadores de Zebu; MATHEUS SILVA MACHADO, Universidade Cruzeiro do Sul; GUILHERME MAGALHÃES MOURA, Instituto Vianna Júnior. |
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Título: |
Programa Nacional de Melhoramento do Gir Leiteiro: sumário brasileiro de touros: 6ª avaliação genômica de touros: resultado do teste de progênie - abril 2023. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Juiz de Fora: Embrapa Gado de Leite, 2023. |
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Páginas: |
126 p. |
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Série: |
(Embrapa Gado de Leite. Documentos, 273). |
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Idioma: |
Português |
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Conteúdo: |
O objetivo do Programa Nacional de Melhoramento do Gir Leiteiro é promover o melhoramento genético da raça Gir por meio de identificação e seleção de animais geneticamente superiores para a produção de leite e seus constituintes, características reprodutivas e de conformação e manejo. |
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Thesagro: |
Bovino; Gado Leiteiro. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1153450/1/Programa-Nacional-de-Melhoramento-do-Gir-Leiteiro-sumario-brasileiro-de-touros.pdf
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Marc: |
LEADER 01324nam a2200325 a 4500
001 2153450
005 2023-06-07
008 2023 bl uuuu 00u1 u #d
100 1 $aPANETTO, J. C. do C.
245 $aPrograma Nacional de Melhoramento do Gir Leiteiro$bsumário brasileiro de touros: 6ª avaliação genômica de touros: resultado do teste de progênie - abril 2023.$h[electronic resource]
260 $aJuiz de Fora: Embrapa Gado de Leite$c2023
300 $a126 p.
490 $a(Embrapa Gado de Leite. Documentos, 273).
520 $aO objetivo do Programa Nacional de Melhoramento do Gir Leiteiro é promover o melhoramento genético da raça Gir por meio de identificação e seleção de animais geneticamente superiores para a produção de leite e seus constituintes, características reprodutivas e de conformação e manejo.
650 $aBovino
650 $aGado Leiteiro
700 1 $aSILVA, M. V. G. B.
700 1 $aVERNEQUE, R. da S.
700 1 $aMACHADO, M. A.
700 1 $aFERNANDES, A. R.
700 1 $aTEIXEIRA, R. B.
700 1 $aMACHADO, C. H. C.
700 1 $aMARTINS, M. F.
700 1 $aREIS, D. R. de L.
700 1 $aBORGES, C. A. V.
700 1 $aOLIVEIRA, J. C. de
700 1 $aVENTURA, H. T.
700 1 $aPEREIRA, M. A.
700 1 $aMACHADO, M. S.
700 1 $aMOURA, G. M.
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Embrapa Gado de Leite (CNPGL) |
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