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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
20/01/2015 |
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Data da última atualização: |
21/06/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BEZERRA, C. A.; MACEDO, L. L. P.; AMORIM, T. M. L.; SANTOS, V. O.; FRAGOSO, R. da R.; LUCENA, W. A.; MENEGUIM, A. M.; VALENCIA-JIMENEZ, A.; ENGLER, G.; SILVA, M. C. M.; ALBUQUERQUE, E. V. S.; GROSSI-DE-SA, M. F. |
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Afiliação: |
FURGS; FURGS; RODRIGO DA ROCHA FRAGOSO, CPAC; WAGNER ALEXANDRE LUCENA, CNPA; IAPAR; UNIVERSITY OF CALDAS; INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE; MARIA CRISTINA MATTAR DA SILVA, CENARGEN; ERIKA VALERIA SALIBA ALBUQUERQUE FR, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN. |
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Título: |
Molecular cloning and characterization of an α-amylase cDNA highly expressed in major feeding stages of the coffee berry borer, Hypothenemus hampei. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Gene, v. 553, n. 1, p. 7-16, Dec. 2014. |
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DOI: |
10.1016/j.gene.2014.09.050 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect a-amylases, including Anthonomus grandis, Ips typographus and Sitophilus oryzae a-amylases. In depth analysis revealed that the highly conserved three amino acid residues (Asp184, Glu220, and Asp285), which compose the catalytic site are also presented in AmyHha amylase. The AmyHha gene seems to be a single copy in the haploid genome and AmyHha transcription levels were found higher in L2 larvae and adult insects, both corresponding to major feeding phases. Modeling of the AmyHha predicted protein uncovered striking structural similarities to the Tenebrio molitor a-amylase also displaying the same amino acid residues involved in enzyme catalysis (Asp184, Glu220 and Asp285). Since AmyHha gene was mostly transcribed in the intestinal tract of H. hampei larvae, the cognate a-amylase could be considered a high valuable target to coffee bean insect control by biotechnological strategies. MenosAbstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect a-amylases, including Anthonomus grandis, Ips typographus and Sitophilus oryzae a-amylases. In depth analysis revealed that the highly conserved three amino acid residues (Asp184, Glu220, and Asp285), which compose the catalytic site are also presented in AmyHha amylase. The AmyHha gene seems to be a single copy in the haploid genome and AmyHha transcription levels were found higher in L2 larvae and adult insects, both corresponding to major feeding phases. Modeling of the AmyHha predicted protein uncovered striking structural similarities to the Tenebrio molitor a-amylase also displaying the same amino acid residues involved in enzyme catalysis (Asp184, Glu220 and Asp285). Since AmyHha gene was mostly transcribed in the intestinal... Mostrar Tudo |
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Palavras-Chave: |
Expressão gênica. |
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Thesagro: |
Amido; Curculionideo; Hidrolise; Praga de planta. |
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Thesaurus Nal: |
Curculionidae; Enzymatic hydrolysis; Gene expression; Insect pests; Starch. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/115999/1/34279.pdf
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Marc: |
LEADER 02795naa a2200385 a 4500
001 2006141
005 2024-06-21
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1016/j.gene.2014.09.050$2DOI
100 1 $aBEZERRA, C. A.
245 $aMolecular cloning and characterization of an α-amylase cDNA highly expressed in major feeding stages of the coffee berry borer, Hypothenemus hampei.$h[electronic resource]
260 $c2014
520 $aAbstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect a-amylases, including Anthonomus grandis, Ips typographus and Sitophilus oryzae a-amylases. In depth analysis revealed that the highly conserved three amino acid residues (Asp184, Glu220, and Asp285), which compose the catalytic site are also presented in AmyHha amylase. The AmyHha gene seems to be a single copy in the haploid genome and AmyHha transcription levels were found higher in L2 larvae and adult insects, both corresponding to major feeding phases. Modeling of the AmyHha predicted protein uncovered striking structural similarities to the Tenebrio molitor a-amylase also displaying the same amino acid residues involved in enzyme catalysis (Asp184, Glu220 and Asp285). Since AmyHha gene was mostly transcribed in the intestinal tract of H. hampei larvae, the cognate a-amylase could be considered a high valuable target to coffee bean insect control by biotechnological strategies.
650 $aCurculionidae
650 $aEnzymatic hydrolysis
650 $aGene expression
650 $aInsect pests
650 $aStarch
650 $aAmido
650 $aCurculionideo
650 $aHidrolise
650 $aPraga de planta
653 $aExpressão gênica
700 1 $aMACEDO, L. L. P.
700 1 $aAMORIM, T. M. L.
700 1 $aSANTOS, V. O.
700 1 $aFRAGOSO, R. da R.
700 1 $aLUCENA, W. A.
700 1 $aMENEGUIM, A. M.
700 1 $aVALENCIA-JIMENEZ, A.
700 1 $aENGLER, G.
700 1 $aSILVA, M. C. M.
700 1 $aALBUQUERQUE, E. V. S.
700 1 $aGROSSI-DE-SA, M. F.
773 $tGene$gv. 553, n. 1, p. 7-16, Dec. 2014.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 8 | |
| 1. |  | UTIAMADA, C. M.; HIGASHI, W. H.; SCHNEIDER, V. P.; SATO, L. M.; YORINORI, J. T. Época de aplicação de fungicida para o controle de mancha alvo (Corynespora cassiicola) em soja. Fitopatologia Brasileira, v. 23, p. 289, ago. 1998. Suplemento, ref. 444. Edição dos Resumos do XXXIII Congresso Brasileiro de Fitopatologia, Belem, ago. 2000.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 2. | | TRÊS, E. F.; GONZAGA, A. C. de O.; BARBOSA, F. R.; LIMA, I. M. de; GASPARETTO, C. A.; SATO, L. M.; CAMPOS, F. T. Artrópodes-pragas e predadores (Arthropoda) associados à cultura do feijoeiro na região de Cristalina-GO. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 3. | | MACHADO, D. L.; ENGEL, V. L.; PODADERA, D. S.; SATO, L. M.; GOEDE, R. G. M. de; MORAES, L. F. D. de; PARROTA, J. A. Edaphic properties drive the effects of vegetation coposition on litter fall and nutrient inputin tropical seasonal forest restoration systems. In: WORLD CONFERENCE ON ECOLOGICAL RESTORATION, 7.; CONGRESO IBEROAMERICANO Y DEL CARIBE DE RESTAURACIÓN ECOLÓGICA, 5.; CONFERÊNCIA BRASILEIRA DE RESTAURAÇÃO ECOLÓGICA, 1., 2017, Foz do Iguassu. Linking science and practice for a better world: book of abstracts. Londrina: Sociedade Brasileira de Restauração Ecológica, 2017. p. 42| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 4. | | ZULIAN, D. F.; SAUL, F. A. C.; OLIVEIRA JÚNIOR, J.; SATO, L. M.; CAMBUIM, J.; CAPO, L. F. M.; MORAES, M. L. T. de; AGUIAR, A. V. de. Proporção sexual de uma população base de Myracrodruon urundeuva em Selvíria, MS. Pesquisa Florestal Brasileira, Colombo, v. 39, (nesp), e201902043, 2019. p. 514. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 6. | | CHOCOBAR, M. L. E.; BELLO, H. J. S.; BASSETTO, C. C.; SILVA-MARINHO, N. M.; SATO, L. M. N.; SPERB, C.; CHAGAS, A. C. de S.; AMARANTE, A. F. T. Biotic potential and pathogenicity of a Haemonchus contortus susceptible laboratory isolate compared to a resistant field isolate in Brazil. Small Ruminant Research, v. 227, oct. 2023, 107063. 10 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 7. | | BARBOSA, F. R.; SILVEIRA, P. M. da; SILVA, C. C. da; GONZAGA, A. C. de O.; QUINTELA, E. D.; LOBO JUNIOR, M.; LIMA, I. M. de; GASPARETTO, C. A.; PRADO, L. de S.; SATO, L. M. Unidades piloto do sistema de produção integrada de feijão comum (Phaseolus vulgaris L.) em plantios irrigados. In: SEMINÁRIO BRASILEIRO DE PRODUÇÃO INTEGRADA DE FRUTAS, 11.; SEMINÁRIO SOBRE SISTEMA AGROPECUÁRIO DE PRODUÇÃO INTEGRADA, 3., 2009, Petrolina. Produção integrada: base de sustentabilidade para a agropecuária brasileira: resumos... Petrolina: Embrapa Semi-Árido, 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 8. | | BARBOSA, F. R.; GONZAGA, A. C. de O.; SILVEIRA, P. M. da; SILVA, C. C. da; QUINTELA, E. D.; LOBO JUNIOR, M.; COBUCCI, T.; LIMA, I. M. de; GASPARETTO, C. A.; PRADO, L. de S.; SATO, L. M. Validação do sistema de produção integrada do feijão comum (Phaseolus vulgaris L.) na região central brasileira. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2010. 8 p. (Embrapa Arroz e Feijão. Circular técnica, 87).| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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