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Registro Completo |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
12/07/1996 |
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Data da última atualização: |
20/02/2008 |
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Autoria: |
ALARSA, C.; CASTRO, S. S. de; FERREIRA, R. P. D. |
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Título: |
Compartimentação morfopedológica como procedimento preparatório para estudo em topossequência - o exemplo de águas de São Pedro, SP. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
IN: CONGRESSO BRASILEIRO DE CIÊNÇIA DO SOLO, 25., 1995, Viçosa, MG. Resumos expandidos. Viçosa: UFV, 1995. v.3, p. 1509-1511. |
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Idioma: |
Português |
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Palavras-Chave: |
Brasil; Morfopedológica; São Paulo; São Pedro; Sistema pedológicos; Topossequência. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00720naa a2200205 a 4500
001 1364845
005 2008-02-20
008 1995 bl uuuu u00u1 u #d
100 1 $aALARSA, C.
245 $aCompartimentação morfopedológica como procedimento preparatório para estudo em topossequência - o exemplo de águas de São Pedro, SP.
260 $c1995
653 $aBrasil
653 $aMorfopedológica
653 $aSão Paulo
653 $aSão Pedro
653 $aSistema pedológicos
653 $aTopossequência
700 1 $aCASTRO, S. S. de
700 1 $aFERREIRA, R. P. D.
773 $tIN: CONGRESSO BRASILEIRO DE CIÊNÇIA DO SOLO, 25., 1995, Viçosa, MG. Resumos expandidos. Viçosa: UFV, 1995.$gv.3, p. 1509-1511.
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Registro original: |
Embrapa Tabuleiros Costeiros (CPATC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
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Data corrente: |
03/03/2015 |
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Data da última atualização: |
10/04/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LADINIG, A.; LUNNEY, J. K.; SOUZA, C. J. H.; ASHLEY, C.; PLASTOW, G.; HARDING, J. C. S. |
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Afiliação: |
ANDREA LADINIG, UNIVERSITY OF SASKATCHEWAN, CANADA; JOAN K. LUNNEY, USDA; CARLOS JOSE HOFF DE SOUZA, CNPASA; CAROLYN ASHLEY, UNIVERSITY OF SASKATCHEWAN, CANADA; GRAHAM PLASTOW, UNIVERSITY OF ALBERTA, CANADA; JOHN C. S. HARDING, UNIVERSITY OF SASKATCHEWAN, CANADA. |
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Título: |
Cytokine profiles in pregnant gilts experimentally infected with porcine reproductive and respiratory syndrome virus and relationships with viral load and fetal outcome. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Veterinary Research, v. 45, 2014. |
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DOI: |
10.1186/s13567-014-0113-8 |
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Idioma: |
Inglês |
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Conteúdo: |
In spite of extensive research, immunologic control mechanisms against Porcine Reproductive and Respiratory Syndrome virus (PRRSv) remain poorly understood. Cytokine responses have been exhaustively studied in nursery pigs and show contradictory results. Since no detailed reports on cytokine responses to PRRSv in pregnant females exist, the objectives of this study were to compare host cytokine responses between PRRSv-infected and non-infected pregnant gilts, and to investigate relationships between cytokine levels in infected gilts and viral load or fetal mortality rate. Serum samples and supernatants of peripheral blood mononuclear cells (PBMC) either stimulated with PRRSv or phorbol myristate acetate/Ionomycin (PMA/Iono) were analyzed for cytokines/chemokines: interleukins (IL) 1-beta (IL1β), IL4, IL8, IL10, IL12, chemokine ligand 2 (CCL2), interferon alpha (IFNα) and interferon gamma (IFNγ). Three cytokines (IFNα, CCL2, IFNγ) in gilt serum differed significantly in inoculated versus control gilts over time. In supernatants of PRRSv stimulated PBMC from PRRSv-infected gilts, levels of IFNα were significantly decreased, while IL8 secretion was significantly increased. PRRSv infection altered the secretion of all measured cytokines, with the exception of IFNα, from PBMC after mitogen stimulation, indicating a possible immunomodulatory effect of PRRSv. IFNα, CCL2, and IFNγ in serum, and IFNγ in supernatants of PMA/Iono stimulated PBMC were significantly associated with viral load in tissues, serum or both. However, only IFNα in supernatants of PRRSv stimulated PBMC was significantly associated with fetal mortality rate. We conclude that of the eight cytokines tested in this study IFNα was the best indicator of viral load and severity of reproductive PRRSv infection. MenosIn spite of extensive research, immunologic control mechanisms against Porcine Reproductive and Respiratory Syndrome virus (PRRSv) remain poorly understood. Cytokine responses have been exhaustively studied in nursery pigs and show contradictory results. Since no detailed reports on cytokine responses to PRRSv in pregnant females exist, the objectives of this study were to compare host cytokine responses between PRRSv-infected and non-infected pregnant gilts, and to investigate relationships between cytokine levels in infected gilts and viral load or fetal mortality rate. Serum samples and supernatants of peripheral blood mononuclear cells (PBMC) either stimulated with PRRSv or phorbol myristate acetate/Ionomycin (PMA/Iono) were analyzed for cytokines/chemokines: interleukins (IL) 1-beta (IL1β), IL4, IL8, IL10, IL12, chemokine ligand 2 (CCL2), interferon alpha (IFNα) and interferon gamma (IFNγ). Three cytokines (IFNα, CCL2, IFNγ) in gilt serum differed significantly in inoculated versus control gilts over time. In supernatants of PRRSv stimulated PBMC from PRRSv-infected gilts, levels of IFNα were significantly decreased, while IL8 secretion was significantly increased. PRRSv infection altered the secretion of all measured cytokines, with the exception of IFNα, from PBMC after mitogen stimulation, indicating a possible immunomodulatory effect of PRRSv. IFNα, CCL2, and IFNγ in serum, and IFNγ in supernatants of PMA/Iono stimul... Mostrar Tudo |
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Thesagro: |
Porco; Reprodução animal; Respiração; Suino; Virus. |
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Thesaurus NAL: |
Cytokines; Porcine reproductive and respiratory syndrome virus; Swine. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/119182/1/cnpasa.pdf
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Marc: |
LEADER 02748naa a2200289 a 4500
001 2010493
005 2018-04-10
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1186/s13567-014-0113-8$2DOI
100 1 $aLADINIG, A.
245 $aCytokine profiles in pregnant gilts experimentally infected with porcine reproductive and respiratory syndrome virus and relationships with viral load and fetal outcome.$h[electronic resource]
260 $c2014
520 $aIn spite of extensive research, immunologic control mechanisms against Porcine Reproductive and Respiratory Syndrome virus (PRRSv) remain poorly understood. Cytokine responses have been exhaustively studied in nursery pigs and show contradictory results. Since no detailed reports on cytokine responses to PRRSv in pregnant females exist, the objectives of this study were to compare host cytokine responses between PRRSv-infected and non-infected pregnant gilts, and to investigate relationships between cytokine levels in infected gilts and viral load or fetal mortality rate. Serum samples and supernatants of peripheral blood mononuclear cells (PBMC) either stimulated with PRRSv or phorbol myristate acetate/Ionomycin (PMA/Iono) were analyzed for cytokines/chemokines: interleukins (IL) 1-beta (IL1β), IL4, IL8, IL10, IL12, chemokine ligand 2 (CCL2), interferon alpha (IFNα) and interferon gamma (IFNγ). Three cytokines (IFNα, CCL2, IFNγ) in gilt serum differed significantly in inoculated versus control gilts over time. In supernatants of PRRSv stimulated PBMC from PRRSv-infected gilts, levels of IFNα were significantly decreased, while IL8 secretion was significantly increased. PRRSv infection altered the secretion of all measured cytokines, with the exception of IFNα, from PBMC after mitogen stimulation, indicating a possible immunomodulatory effect of PRRSv. IFNα, CCL2, and IFNγ in serum, and IFNγ in supernatants of PMA/Iono stimulated PBMC were significantly associated with viral load in tissues, serum or both. However, only IFNα in supernatants of PRRSv stimulated PBMC was significantly associated with fetal mortality rate. We conclude that of the eight cytokines tested in this study IFNα was the best indicator of viral load and severity of reproductive PRRSv infection.
650 $aCytokines
650 $aPorcine reproductive and respiratory syndrome virus
650 $aSwine
650 $aPorco
650 $aReprodução animal
650 $aRespiração
650 $aSuino
650 $aVirus
700 1 $aLUNNEY, J. K.
700 1 $aSOUZA, C. J. H.
700 1 $aASHLEY, C.
700 1 $aPLASTOW, G.
700 1 $aHARDING, J. C. S.
773 $tVeterinary Research$gv. 45, 2014.
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Embrapa Pesca e Aquicultura (CNPASA) |
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