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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
26/07/2001 |
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Data da última atualização: |
26/07/2001 |
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Autoria: |
FELSKE, A.; WOLTERINK, A.; VAN LIS, R.; DE VOS, W. M.; AKKERMANS, A. D. L. |
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Título: |
Response of a soil bacterial community to grassland succession as monitored by 16S rRNA clevels of the predominant ribotypes. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Applied and Environmental Microbiology, Washington, v. 66, n. 9, p. 3998-4003, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
The composition of predominant soll bacteria during grassland succession was investigated in the Dutch Drentse A area. Five meadows, taken out of agricultural at different time points, and one currently fertitlized plot represented different stages of grassland succession. Since fertilization and agricultural production were stopped, the six plots showed a constant decline in the levels of nutrients and vegetation changes. The activity of the predominant bacteria was monitored by direct ribosome isolation from soil and temperature gradient gel electrophoresis of reverse transcription (RT)-PCR products generated from bacterial 16S rRNA. The amounts of 16S rRNA of 20 predominant ribosome types per gram of soil were monitored via multiple competitive RT-PCR in six plots at different succession stages. These ribosome types mainly represented Bacillus and members of the Acidobacterium cluster and the @ subclass of the Proteobacteria. The 20 16S rRNA molecules monitored approximately half of all bacterial soil rRNA which was estimated by dot blot hybridizations of soil rRNA with the Bacteria probe EUB338. The grasslands showed highly reproducible and specific shifts of bacterial ribosome type composition. The total bacterial ribosome level increased during the first years after agricultural production and fertilization stopped. This correlated with the collapse of the dominant Lolium population and an increased rate of mineralization of organic matter. The results indicate that is a true correlation between the total activity of the bacterial community in soil and the amount of bacterial ribosomes. MenosThe composition of predominant soll bacteria during grassland succession was investigated in the Dutch Drentse A area. Five meadows, taken out of agricultural at different time points, and one currently fertitlized plot represented different stages of grassland succession. Since fertilization and agricultural production were stopped, the six plots showed a constant decline in the levels of nutrients and vegetation changes. The activity of the predominant bacteria was monitored by direct ribosome isolation from soil and temperature gradient gel electrophoresis of reverse transcription (RT)-PCR products generated from bacterial 16S rRNA. The amounts of 16S rRNA of 20 predominant ribosome types per gram of soil were monitored via multiple competitive RT-PCR in six plots at different succession stages. These ribosome types mainly represented Bacillus and members of the Acidobacterium cluster and the @ subclass of the Proteobacteria. The 20 16S rRNA molecules monitored approximately half of all bacterial soil rRNA which was estimated by dot blot hybridizations of soil rRNA with the Bacteria probe EUB338. The grasslands showed highly reproducible and specific shifts of bacterial ribosome type composition. The total bacterial ribosome level increased during the first years after agricultural production and fertilization stopped. This correlated with the collapse of the dominant Lolium population and an increased rate of mineralization of organic matter. The results indicate tha... Mostrar Tudo |
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Palavras-Chave: |
Ribossomo. |
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Thesagro: |
Bactéria; Pastagem; Solo. |
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Thesaurus Nal: |
grasslands; ribosomes; soil. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02349naa a2200253 a 4500
001 1621226
005 2001-07-26
008 2000 bl --- 0-- u #d
100 1 $aFELSKE, A.
245 $aResponse of a soil bacterial community to grassland succession as monitored by 16S rRNA clevels of the predominant ribotypes.
260 $c2000
520 $aThe composition of predominant soll bacteria during grassland succession was investigated in the Dutch Drentse A area. Five meadows, taken out of agricultural at different time points, and one currently fertitlized plot represented different stages of grassland succession. Since fertilization and agricultural production were stopped, the six plots showed a constant decline in the levels of nutrients and vegetation changes. The activity of the predominant bacteria was monitored by direct ribosome isolation from soil and temperature gradient gel electrophoresis of reverse transcription (RT)-PCR products generated from bacterial 16S rRNA. The amounts of 16S rRNA of 20 predominant ribosome types per gram of soil were monitored via multiple competitive RT-PCR in six plots at different succession stages. These ribosome types mainly represented Bacillus and members of the Acidobacterium cluster and the @ subclass of the Proteobacteria. The 20 16S rRNA molecules monitored approximately half of all bacterial soil rRNA which was estimated by dot blot hybridizations of soil rRNA with the Bacteria probe EUB338. The grasslands showed highly reproducible and specific shifts of bacterial ribosome type composition. The total bacterial ribosome level increased during the first years after agricultural production and fertilization stopped. This correlated with the collapse of the dominant Lolium population and an increased rate of mineralization of organic matter. The results indicate that is a true correlation between the total activity of the bacterial community in soil and the amount of bacterial ribosomes.
650 $agrasslands
650 $aribosomes
650 $asoil
650 $aBactéria
650 $aPastagem
650 $aSolo
653 $aRibossomo
700 1 $aWOLTERINK, A.
700 1 $aVAN LIS, R.
700 1 $aDE VOS, W. M.
700 1 $aAKKERMANS, A. D. L.
773 $tApplied and Environmental Microbiology, Washington$gv. 66, n. 9, p. 3998-4003, 2000.
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Embrapa Agrobiologia (CNPAB) |
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