Data/hora: 19/06/2024 09:07:23

Biblioteca(s): 	Embrapa Soja.	
Data corrente: 	11/09/2006	
Data da última atualização: 	11/09/2006	
Autoria: 	KOWALCHUCK, G. A.; BRUIJN, F. J. de; HEAD, I. M.; AKKERMANS, A. D. L.; ELSAS, J. D. van (Ed.).	
Título: 	Molecular microbial ecology manual.	
Edição: 	2. ed.	
Ano de publicação: 	2004	
Fonte/Imprenta: 	Dordrecht: Kluwer, 2004.	
Volume: 	2v.	
Idioma: 	Inglês	
Conteúdo: 	Simplified protocols for the preparation of genomic DNA from  bacterial cultures; Extraction of ribosomal RNA from microbial cultures; Extraction of microbial DNA from aquatic sources: marine environments; Extraction of microbial DNA from aquatic sources: freshwater; Methods for extracting DNA from microbial mats and cultivated micro-organisms: high molecular weight DNA from  French press lysis; Extraction of microbial DNA from aquatic sediments; Extraction of microbial RNA from aquatic sources: marine environments; Extraction of total RNA and DNA from bacterioplankton; Methods for extracting RNA or ribosomes from microbial mats and cultivated microorganisms; Cell extraction method; DNA and RNA extraction from soil;  Rapid simultaneous extraction of DNA and RNA from bulk and rhizosphere soil; Direct extraction of fungal DNA from soil; Purification of microbial genes from soil and rhizosphere by magnetic capture hybridization and subsequent amplification of target genes by PCR; Direct ribosome isolation from soil; DNA extraction from actinorhizal nodules; Quantification of nucleic acids; Quantification of nucleic acids from aquatic environments by using green-fluorescent dyes and microtiter plates; Degradation and turnover of extracellular DNA in marine sediments;  Incorporation of thymidine into DNA of soil bacteria; Preparation of radioactive probes; Detection of nucleic ccids by chemiluminescence; Parameters of nucleic acid hybridization experiments; Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization; Detection of microbial DNA sequences by colony hybridization; Polymerase chain reaction analysis of soil microbial DNA; Detection of microbial nucleic acids by polymerase chain reaction in aquatic samples; Isolation and detection of bacterial DNA sequences in dairy products; Quantitative PCR of environmental samples; Molecular beacons for homogeneous real-time monitoring of amplification products; Detection and enumeration of soil bacteria using the MPN-PCR technique; Detection of mRNA and rRNA via reverse transcription and PCR in soil; Amplification of ribosomal RNA sequences;  Cloning 16S rRNA genes and utilization to type bacterial communities; SARST, Serial Analysis of Ribosomal Sequence Tags; Oligonucleotide Fingerprinting of Ribosomal RNA Genes (OFRG); Genotyping of bacterial isolates from the environment using low-molecular-weight RNA fingerprints; Characterization of the diversity of ecologically important microbes by rep-PCR genomic fingerprinting; Genomic fingerprinting of micro-organisms by AFLPTM and ERIC-anchor PCR; The use of pulsed-field gel electrophoresis to study bacteria recovered from the environment; Easy individual strain and community typing by rDNA ITS1 analysis; In situ PCR methodologies for visualization of microscale genetic and taxonomic diversities of prokaryotic communities; Sensitive multi-color fluorescence in situ hybridization for the identification of environmental microorganisms; Use of Cloned Artificial Targets for FISH (catFISH) for the optimization of oligonucleotide probe hybridization conditions with 16S rRNA clones for in situ quantification of uncultivated prokaryotic cells; Denaturing gradient gel electrophoresis (DGGE) in microbial ecology; Fungal community analysis using PCR- Denaturing Gradient Gel Electrophoresis (DGGE); The Analysis of Microbial Communities with Terminal Restriction Fragment Length Polymorphism (T-RFLP) Microbial community analysis by PCR-single-strand conformation polymorphism (PCR-SSCP); Isolation of high molecular weight genomic DNA from soil bacteria for genomic library construction; Use of Biolog r _ for the Community Level Physiological Profiling (CLPP) of environmental samples; Fluorescent staining of microbes for total direct counts; Detection of microbes by Scanning Confocal Laser Microscopy (SCLM); Production of anti-microbial antibodies and their utilization in studies of microbial autecology by immunofluorescence microscopy and in situ CMEIAS image analysis; The slide immunoenzymatic assay (SIA): A simple and low cost system suitable for detecting water-borne microbes without the need for sophisticated technological infrastructure; In situ hybridization to detect microbial messenger RNA in plant tissues; Fatty acid analysis in the identification, taxonomy and ecology of (plant pathogenic) bacteria; Determination of microbial community structure using phospholipid fatty acid profiles; Respiratory lipoquinones as biomarkers; Environmental proteomics: methods and applications for aquatic ecosystems; Natural transformation in aquatic environments; Natural transformation in soil: microcosm studies; Plasmid transfer in aquatic environments; Conjugation in the epilithon; Detection of bacterial conjugation in soil; Transduction in the aquatic environment; Phage ecology and genetic exchange in soil; Lac as a marker gene to track microbes in the environment; XylE as a marker gene for microorganisms; GUS as a marker to track microbes; The celB marker gene; Visualisation of microbes and their interactions in the rhizosphere using auto fluorescent proteins as markers; Identification of bacteria by their intrinsic sequences: probe design and testing of their specificity; Subtraction hybridization for the production of high specificity DNA probes; Considerations for the use of functional markers and field release of genetically engineered microorganisms to soils and plants; Application of ecological diversity statistics in microbial ecology; Sampling efficiency and interpretation of diversity in 16S rRNA gene libraries; LIBSHUFF comparisons of 16S rRNA gene clone libraries; Cluster analysis and statistical comparison of molecular community profile data; Computer-assisted analysis of molecular fingerprint profiles and database construction; Multivariate statistical methods and artificial neural networks for analysis of microbial community molecular fingerprints; Quantitative fluorescence in situ hybridisation (FISH): statistical methods for valid cell counting; Oligonucleotide probe design for mixed microbial community microarrays and other applications and important considerations for data analysis; Design of microarrays for genome-wide expression profiling; Assessment of the membrane potential, intracellular pH and respiration of bacteria employing fluorescence techniques; Use of microelectrodes to measure in situ microbial activities in biofilms, sediments, and microbial mats; Application of whole-cell biosensors in soil; Detection of bacterial homoserine lactone quorum sensing signals; BrdU substrate utilization assay; Stable isotope probing of nucleic acids to identify active microbial populations; Linking microbial community structure and functioning: stable isotope (13C) labeling in combination with PLFA analysis; Correlating single-cell count with function in mixed natural microbial communities through STARFISH; Differential display of mRNA; Macro-arrays protocols for gene expression studies in bacteria; Oligonucleotide-based functional gene arrays for analysis of microbial communities in the environment; Proteomic analysis of bacterial systems.	
Thesagro: 	Bactéria; Microrganismo; Solo.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Soja (CNPSO)	
Exemplares: 	CNPSO - (26617-1) - ADC - LV - -- - 579.334 - K88m - 2006.00151 - DIS
CNPSO - (26617-2) - ADC - LV - -- - 579.334 - K88m - 2006.00152 - DIS