|
|
Registro Completo |
Biblioteca(s): |
Embrapa Rondônia. |
Data corrente: |
15/03/2016 |
Data da última atualização: |
15/03/2016 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
RAMOS, R. da M.; COSTA, R. S. C. da; PASSOS, A. M. A. dos; AKER, A. M.; SOBRAL, I. M.; LENA, A. G. |
Afiliação: |
Ronaldo da Mota Ramos, FIMCA; ROGERIO SEBASTIAO CORREA DA COSTA, CPAF-RO; ALEXANDRE MARTINS ABDAO DOS PASSOS, CPAF-RO; Andréia Marcilane Aker, UNIR; Ito Medeiros Sobral, FIMCA; ALDOIR GUILHERME LENA, CPAF-RO. |
Título: |
Fungos micorrízicos arbusculares em plantas de cobertura utilizadas no plantio direto. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
In: ENCONTRO DE INICIAÇÃO À PESQUISA DA EMBRAPA RONDÔNIA, 6.; ENCONTRO DE PÓS-GRADUAÇÃO, 2015, Porto Velho, RO. Anais... Porto Velho: Embrapa Rondônia, 2015. p. 88. |
Idioma: |
Português |
Conteúdo: |
O trabalho tem como objetivo monitorar a influência das plantas de coberturas na população dos fungos micorrízicos arbusculares, em um sistema de plantio direto no campo experimental da Embrapa Rondônia, em Porto Velho. |
Palavras-Chave: |
FMA. |
Thesagro: |
Cobertura do Solo; Simbiose. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141221/1/VI-EIPER-Micorrizas.pdf
|
Marc: |
LEADER 00954nam a2200205 a 4500 001 2041050 005 2016-03-15 008 2015 bl uuuu u00u1 u #d 100 1 $aRAMOS, R. da M. 245 $aFungos micorrízicos arbusculares em plantas de cobertura utilizadas no plantio direto.$h[electronic resource] 260 $aIn: ENCONTRO DE INICIAÇÃO À PESQUISA DA EMBRAPA RONDÔNIA, 6.; ENCONTRO DE PÓS-GRADUAÇÃO, 2015, Porto Velho, RO. Anais... Porto Velho: Embrapa Rondônia, 2015. p. 88.$c2015 520 $aO trabalho tem como objetivo monitorar a influência das plantas de coberturas na população dos fungos micorrízicos arbusculares, em um sistema de plantio direto no campo experimental da Embrapa Rondônia, em Porto Velho. 650 $aCobertura do Solo 650 $aSimbiose 653 $aFMA 700 1 $aCOSTA, R. S. C. da 700 1 $aPASSOS, A. M. A. dos 700 1 $aAKER, A. M. 700 1 $aSOBRAL, I. M. 700 1 $aLENA, A. G.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Rondônia (CPAF-RO) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
03/09/2021 |
Data da última atualização: |
03/09/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
PAES, B. G.; STEINDORF, A. S.; FORMIGHIERI, E. F.; PEREIRA, I. S.; ALMEIDA, J. R. M. de. |
Afiliação: |
BARBARA G. PAES, Universidade de Brasília; ANDREI STECCA STEINDORF, Universidade de Brasília; EDUARDO FERNANDES FORMIGHIERI, CNPAE; ILDINETE SILVA PEREIRA, Universidade de Brasília; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE. |
Título: |
Physiological characterization and transcriptome analysis of Pichia pastoris reveals its response to lignocellulose-derived inhibitors. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
AMB Express, v. 11, n. 2, 2021. |
Descrição Física: |
PDF: il. color. |
DOI: |
https://doi.org/10.1186/s13568-020-01170-9 |
Idioma: |
Inglês |
Conteúdo: |
The negative efects of lignocellulose-derived inhibitors such as acetic acid and furaldehydes on microbial metabolism constitute a signifcant drawback to the usage of biomass feedstocks for the production of fuels and chemicals. The yeast Pichia pastoris has shown a great biotechnological potential for producing heterologous proteins and renewable chemicals. Despite its relevance, the performance of P. pastoris in presence of lignocellulose-derived inhibitors remains unclear. In this work, our results show for the frst time the dose-dependent response of P. pastoris to acetic acid, furaldehydes (HMF and furfural), and sugarcane biomass hydrolysate, both at physiological and transcriptional levels. The yeast was able to grow in synthetic media with up to 6 g.L-1 acetic acid, 1.75 g.L-1 furaldehydes or hydrolysate diluted to 10% (v/v). However, its metabolism was completely hindered in presence of hydrolysate diluted to 30% (v/v). Additionally, the yeast was capable to co-consume acetic acid and glucose. At the transcriptional level, P. pastoris response to lignocellulose-derived inhibitors relays on the up-regulation of genes related to transmembrane transport, oxidoreductase activities, RNA processing, and the repression of pathways related to biosynthetic processes and central carbon metabolism. These results demonstrate a polygenetic response that involves detoxifcation activi ties, and maintenance of energy and cellular homeostasis. In this context, ALD4, OYE3, QOR2, NTL100, YCT1, and PPR1 were identifed as target genes to improve P. pastoris tolerance. Altogether, this work provides valuable insights into the P. pastoris stress tolerance, which can be useful to expand its use in diferent bioprocesses. MenosThe negative efects of lignocellulose-derived inhibitors such as acetic acid and furaldehydes on microbial metabolism constitute a signifcant drawback to the usage of biomass feedstocks for the production of fuels and chemicals. The yeast Pichia pastoris has shown a great biotechnological potential for producing heterologous proteins and renewable chemicals. Despite its relevance, the performance of P. pastoris in presence of lignocellulose-derived inhibitors remains unclear. In this work, our results show for the frst time the dose-dependent response of P. pastoris to acetic acid, furaldehydes (HMF and furfural), and sugarcane biomass hydrolysate, both at physiological and transcriptional levels. The yeast was able to grow in synthetic media with up to 6 g.L-1 acetic acid, 1.75 g.L-1 furaldehydes or hydrolysate diluted to 10% (v/v). However, its metabolism was completely hindered in presence of hydrolysate diluted to 30% (v/v). Additionally, the yeast was capable to co-consume acetic acid and glucose. At the transcriptional level, P. pastoris response to lignocellulose-derived inhibitors relays on the up-regulation of genes related to transmembrane transport, oxidoreductase activities, RNA processing, and the repression of pathways related to biosynthetic processes and central carbon metabolism. These results demonstrate a polygenetic response that involves detoxifcation activi ties, and maintenance of energy and cellular homeostasis. In this context, ALD4, OYE3, QOR2, NTL1... Mostrar Tudo |
Palavras-Chave: |
Furaldehydes; Komagataella phaffi; Lignocellulosic hydrolysate. |
Thesaurus NAL: |
Acetic acid; Pichia pastoris; Stress tolerance. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/225706/1/Physiological-characterization-and-transcriptome-analysis-2021.pdf
|
Marc: |
LEADER 02579naa a2200265 a 4500 001 2134061 005 2021-09-03 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1186/s13568-020-01170-9$2DOI 100 1 $aPAES, B. G. 245 $aPhysiological characterization and transcriptome analysis of Pichia pastoris reveals its response to lignocellulose-derived inhibitors.$h[electronic resource] 260 $c2021 300 $cPDF: il. color. 520 $aThe negative efects of lignocellulose-derived inhibitors such as acetic acid and furaldehydes on microbial metabolism constitute a signifcant drawback to the usage of biomass feedstocks for the production of fuels and chemicals. The yeast Pichia pastoris has shown a great biotechnological potential for producing heterologous proteins and renewable chemicals. Despite its relevance, the performance of P. pastoris in presence of lignocellulose-derived inhibitors remains unclear. In this work, our results show for the frst time the dose-dependent response of P. pastoris to acetic acid, furaldehydes (HMF and furfural), and sugarcane biomass hydrolysate, both at physiological and transcriptional levels. The yeast was able to grow in synthetic media with up to 6 g.L-1 acetic acid, 1.75 g.L-1 furaldehydes or hydrolysate diluted to 10% (v/v). However, its metabolism was completely hindered in presence of hydrolysate diluted to 30% (v/v). Additionally, the yeast was capable to co-consume acetic acid and glucose. At the transcriptional level, P. pastoris response to lignocellulose-derived inhibitors relays on the up-regulation of genes related to transmembrane transport, oxidoreductase activities, RNA processing, and the repression of pathways related to biosynthetic processes and central carbon metabolism. These results demonstrate a polygenetic response that involves detoxifcation activi ties, and maintenance of energy and cellular homeostasis. In this context, ALD4, OYE3, QOR2, NTL100, YCT1, and PPR1 were identifed as target genes to improve P. pastoris tolerance. Altogether, this work provides valuable insights into the P. pastoris stress tolerance, which can be useful to expand its use in diferent bioprocesses. 650 $aAcetic acid 650 $aPichia pastoris 650 $aStress tolerance 653 $aFuraldehydes 653 $aKomagataella phaffi 653 $aLignocellulosic hydrolysate 700 1 $aSTEINDORF, A. S. 700 1 $aFORMIGHIERI, E. F. 700 1 $aPEREIRA, I. S. 700 1 $aALMEIDA, J. R. M. de 773 $tAMB Express$gv. 11, n. 2, 2021.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Agroenergia (CNPAE) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|