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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
03/01/2019 |
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Data da última atualização: |
27/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AGUSTINI, B. C.; SILVA, G. A. da; BOMFIM, T. M. B. B. |
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Afiliação: |
BRUNA CARLA AGUSTINI, CNPUV; GILDO ALMEIDA DA SILVA, CNPUV; Tania Maria Bordin Bonfim, Institute of Microbiology, Academy of Sciences of the Czech Republic. |
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Título: |
MALDI-TOF MS Supplementary database for species identification employing the yeast diversity encountered on southern Brazil grapes. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Folia Microbiológica, v. 63, p. 685-693, 2018. |
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DOI: |
https://doi.org/10.1007/s12223-018-0607-2 |
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Idioma: |
Inglês |
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Conteúdo: |
The study of grape microflora is of interest when autochthonous yeasts, which are related to typical wine characteristics, are intended to be used in winemaking. The election of matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) as the first method for yeast identification was based on its accuracy and rapidity compared to alternative laboratory protocols for identification. The aims of this study are to consolidate the MALDI-TOF MS Supplementary database for environmental yeasts already constructed, to expand it through the addition of standard spectra of not included yeast species, and to discuss the grape microflora encountered in Southern Brazil. A total of 358 strains, isolated from grape berries, were submitted to protein profiling employing Biotyper and Supplementary database. Molecular biology techniques were used as alternatives to identify 6.4% of strains not promptly designated by protein profiling. These strains corresponded to the species Candida californica, Zygoascus meyerae, Candida akabanensis, Candida azyma, and Hanseniaspora vineae. The MALDI-TOF MS spectra of the identified species were added to Supplementary database. The presented results strengthen the need for further expansion of the mass spectra database to broaden its microbiological application. |
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Palavras-Chave: |
Candida akabanensis; Candida azyma; Candida californica; Grape microflora; MALDI-TOF MS spectra; Zygoascus meyerae. |
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Thesaurus Nal: |
Grapes; Hanseniaspora vineae; Strains. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/189666/1/MALDI-TOF-MS-Supplementary-database-for-species-identification-employing-the-yeast-diversity-encountered-on-southern-Brazil-grapes-s12223-018-0607-2.pdf
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Marc: |
LEADER 02182naa a2200265 a 4500
001 2102899
005 2019-04-27
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s12223-018-0607-2$2DOI
100 1 $aAGUSTINI, B. C.
245 $aMALDI-TOF MS Supplementary database for species identification employing the yeast diversity encountered on southern Brazil grapes.$h[electronic resource]
260 $c2018
520 $aThe study of grape microflora is of interest when autochthonous yeasts, which are related to typical wine characteristics, are intended to be used in winemaking. The election of matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) as the first method for yeast identification was based on its accuracy and rapidity compared to alternative laboratory protocols for identification. The aims of this study are to consolidate the MALDI-TOF MS Supplementary database for environmental yeasts already constructed, to expand it through the addition of standard spectra of not included yeast species, and to discuss the grape microflora encountered in Southern Brazil. A total of 358 strains, isolated from grape berries, were submitted to protein profiling employing Biotyper and Supplementary database. Molecular biology techniques were used as alternatives to identify 6.4% of strains not promptly designated by protein profiling. These strains corresponded to the species Candida californica, Zygoascus meyerae, Candida akabanensis, Candida azyma, and Hanseniaspora vineae. The MALDI-TOF MS spectra of the identified species were added to Supplementary database. The presented results strengthen the need for further expansion of the mass spectra database to broaden its microbiological application.
650 $aGrapes
650 $aHanseniaspora vineae
650 $aStrains
653 $aCandida akabanensis
653 $aCandida azyma
653 $aCandida californica
653 $aGrape microflora
653 $aMALDI-TOF MS spectra
653 $aZygoascus meyerae
700 1 $aSILVA, G. A. da
700 1 $aBOMFIM, T. M. B. B.
773 $tFolia Microbiológica$gv. 63, p. 685-693, 2018.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
25/09/1998 |
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Data da última atualização: |
13/07/2018 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
CARVALHO, H. W. L. de; SANTOS, M. X. dos; LEAL, M. de L. da S.; MONTEIRO, A. A. T.; LIRA, M. A.; CARVALHO, B. C. L. de. |
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Afiliação: |
EMBRAPA/CNPMS. |
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Título: |
Comportamento de cultivares de milho no Nordeste Brasileiro no ano de 1997. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE MILHO E SORGO, 22., 1998, Recife. Globalização e segurança alimentar: anais. Recife: ABMS, 1998. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Palavras-Chave: |
Brasil; Maize; Nordeste; Northeast; Variedades. |
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Thesagro: |
Melhoramento; Milho; Zea Mays. |
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Thesaurus NAL: |
Brazil; breeding; varieties. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/39315/1/Comportamento-cultivares.pdf
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Marc: |
LEADER 00887nam a2200301 a 4500
001 1480611
005 2018-07-13
008 1998 bl uuuu u00u1 u #d
100 1 $aCARVALHO, H. W. L. de
245 $aComportamento de cultivares de milho no Nordeste Brasileiro no ano de 1997.$h[electronic resource]
260 $aIn: CONGRESSO NACIONAL DE MILHO E SORGO, 22., 1998, Recife. Globalização e segurança alimentar: anais. Recife: ABMS$c1998
300 $c1 CD-ROM.
650 $aBrazil
650 $abreeding
650 $avarieties
650 $aMelhoramento
650 $aMilho
650 $aZea Mays
653 $aBrasil
653 $aMaize
653 $aNordeste
653 $aNortheast
653 $aVariedades
700 1 $aSANTOS, M. X. dos
700 1 $aLEAL, M. de L. da S.
700 1 $aMONTEIRO, A. A. T.
700 1 $aLIRA, M. A.
700 1 $aCARVALHO, B. C. L. de
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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