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Registro Completo |
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
09/02/2021 |
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Data da última atualização: |
09/02/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BENEMANN, D. de P.; CECHIN, J.; AGOSTINETTO, D.; VARGAS, L. |
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Afiliação: |
DAIANE DE PINHO BENEMANN, Department of Crop Protection, Federal University of Pelotas, PO Box 354, Capão do Leão -RS, Brazil, Zip Code 96010-900; JOANEI CECHIN, 1Department of Crop Protection, Federal University of Pelotas, PO Box 354, Capão do Leão -RS, Brazil, Zip Code 96010-900; DIRCEU AGOSTINETTO, 1Department of Crop Protection, Federal University of Pelotas, PO Box 354, Capão do Leão -RS, Brazil, Zip Code 96010-900; LEANDRO VARGAS, CNPT. |
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Título: |
Expression of antioxidant genes and photosynthetic apparatus in the soybean crop in competition with Italian ryegrass biotypes. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Australian Journal Of Crop Science, v. 14, p. 718-728, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Crop-weed competition induces stress in plants causing physiological changes which can be evaluated using the RT-qPCR technique. The aim of this study was to evaluate the stability of candidate reference genes and measure the relative gene expression of antioxidant enzymes and components of the photosynthetic apparatus in soybean in competition with glyphosate-susceptible and resistant Italian ryegrass (Lolium multiflorum Lam.). The experiment was carried out in a greenhouse using replacement series between soybean and Italian ryegrass biotypes. In this study, candidate reference genes were evaluated for use as controls in RT-qPCR to quantify gene expression. An evaluation was made of genes that encode catalase, ascorbate peroxidase, superoxide dismutase, chlorophyll a/b, phytochrome A and cytochrome P450 at 50 days after emergence of the soybean. The SKIP and GAPDH genes were the most stable for soybean and Italian ryegrass, respectively. The soybean subjected to the interspecific competition with the glyphosate-resistant Italian ryegrass biotype showed an increase in superoxide dismutase gene expression. The catalase and cytochrome P450 genes were up-regulated in the susceptible biotype while the other genes were down-regulated. However, the soybean crop under interspecific competition with glyphosate-resistant biotye showed as up-regulated for all the genes evaluated. For the photosynthetic apparatus, cytochrome P450 gene was up-regulated under intraspecific competition on both Italian ryegrass biotypes, while the phytochrome A was up-regulated only in the resistant biotype. Thus, the increase in genes investigated represents a potential tool for the genetic improvement of plants to enhance their competitive capacity. MenosCrop-weed competition induces stress in plants causing physiological changes which can be evaluated using the RT-qPCR technique. The aim of this study was to evaluate the stability of candidate reference genes and measure the relative gene expression of antioxidant enzymes and components of the photosynthetic apparatus in soybean in competition with glyphosate-susceptible and resistant Italian ryegrass (Lolium multiflorum Lam.). The experiment was carried out in a greenhouse using replacement series between soybean and Italian ryegrass biotypes. In this study, candidate reference genes were evaluated for use as controls in RT-qPCR to quantify gene expression. An evaluation was made of genes that encode catalase, ascorbate peroxidase, superoxide dismutase, chlorophyll a/b, phytochrome A and cytochrome P450 at 50 days after emergence of the soybean. The SKIP and GAPDH genes were the most stable for soybean and Italian ryegrass, respectively. The soybean subjected to the interspecific competition with the glyphosate-resistant Italian ryegrass biotype showed an increase in superoxide dismutase gene expression. The catalase and cytochrome P450 genes were up-regulated in the susceptible biotype while the other genes were down-regulated. However, the soybean crop under interspecific competition with glyphosate-resistant biotye showed as up-regulated for all the genes evaluated. For the photosynthetic apparatus, cytochrome P450 gene was up-regulated under intraspecific competition o... Mostrar Tudo |
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Palavras-Chave: |
Antioxidant enzymes; Candidate reference genes; Physiological changes. |
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Thesaurus Nal: |
Plant competition. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/221021/1/Beneman-2020-718.pdf
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Marc: |
LEADER 02425naa a2200205 a 4500
001 2129875
005 2021-02-09
008 2020 bl uuuu u00u1 u #d
100 1 $aBENEMANN, D. de P.
245 $aExpression of antioxidant genes and photosynthetic apparatus in the soybean crop in competition with Italian ryegrass biotypes.$h[electronic resource]
260 $c2020
520 $aCrop-weed competition induces stress in plants causing physiological changes which can be evaluated using the RT-qPCR technique. The aim of this study was to evaluate the stability of candidate reference genes and measure the relative gene expression of antioxidant enzymes and components of the photosynthetic apparatus in soybean in competition with glyphosate-susceptible and resistant Italian ryegrass (Lolium multiflorum Lam.). The experiment was carried out in a greenhouse using replacement series between soybean and Italian ryegrass biotypes. In this study, candidate reference genes were evaluated for use as controls in RT-qPCR to quantify gene expression. An evaluation was made of genes that encode catalase, ascorbate peroxidase, superoxide dismutase, chlorophyll a/b, phytochrome A and cytochrome P450 at 50 days after emergence of the soybean. The SKIP and GAPDH genes were the most stable for soybean and Italian ryegrass, respectively. The soybean subjected to the interspecific competition with the glyphosate-resistant Italian ryegrass biotype showed an increase in superoxide dismutase gene expression. The catalase and cytochrome P450 genes were up-regulated in the susceptible biotype while the other genes were down-regulated. However, the soybean crop under interspecific competition with glyphosate-resistant biotye showed as up-regulated for all the genes evaluated. For the photosynthetic apparatus, cytochrome P450 gene was up-regulated under intraspecific competition on both Italian ryegrass biotypes, while the phytochrome A was up-regulated only in the resistant biotype. Thus, the increase in genes investigated represents a potential tool for the genetic improvement of plants to enhance their competitive capacity.
650 $aPlant competition
653 $aAntioxidant enzymes
653 $aCandidate reference genes
653 $aPhysiological changes
700 1 $aCECHIN, J.
700 1 $aAGOSTINETTO, D.
700 1 $aVARGAS, L.
773 $tAustralian Journal Of Crop Science$gv. 14, p. 718-728, 2020.
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Registro original: |
Embrapa Trigo (CNPT) |
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| Registros recuperados : 163 | |
| 22. |  | VARGAS, L.; GAZZIERO, D. P.; AGOSTINETTO, D.; KARAM, D. Como manejar. Caderno Técnico Cultivar, Pelotas, v. 13, n, 146, p. 10-11, jul. 2011. Encarte.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 23. | | VARGAS, L.; SCHNEIDER, T.; AGOSTINETTO, D.; BIANCHI, M. A. Geographic distribution of ryegrass resistent to the clethodim herbicide in Rio Grande do Sul. Planta Daninha, Viçosa, MG, v. 34, n. 2, p. 365-373, Apr./June 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Trigo. |
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| 28. | | CECHIN, J.; RUCHEL, Q.; SCHNEIDER, T.; VARGAS, L.; AGOSTINETTO, D. Resistencia de Raphanus sativus L. ao herbicida nicosulfurom. In: CONGRESO LATINOAMERICANO DE MALEZAS, 22.; CONGRESO ARGENTINO DE MALEZAS, 1., Buenos Aires, 2015. Malezas 2015: ciência y producción: hacia um manejo racional: [anales]. [Buenos Aires]: Asociación Latinoamericana de Malezas: Asociación Argentina de Ciencia de las Malezas, 2015. Trabajos científicos, RH.EP.03.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 30. | | PAULA, J. de; DAL MAGRO, T.; VARGAS, L.; AGOSTINETTO, D. Eficiência de herbicidas aplicados em pré-semeadura da soja, para o controle de buva. In: CONGRESSO BRASILEIRO DA CIÊNCIA DAS PLANTAS DANINHAS, 26.; CONGRESO DE LA ASOCIACION LATINOAMERICANA DE MALEZAS, 18., 2008, Ouro Preto. A ciência das plantas daninhas na sustentabilidade dos sistemas agrícolas: palestras apresentadas. Sete Lagoas: SBCPD: Embrapa Milho e Sorgo, 2008. 1 CD-ROM. 5 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| Registros recuperados : 163 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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