Data/hora: 08/06/2024 01:19:01

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 30/03/1994
Data da última atualização: | 30/03/1994
Autoria: | AFZA, R.; KAMMER, D.; DOLEZEL, J.; KONIG, J.; DUREN, M. van; KAHL, G. NOVAK, F. J.
Título: | The potential of nuclear DNA flow - cytometry and DNA fingerprinting for Musa inprovement programmes.
Ano de publicação: | 1992
Fonte/Imprenta: | Montpellier, France: CIRAD-FLHOR, 1992.
Páginas: | P.65-75.
ISBN: | 2-87614-108-6
Idioma: | Inglês
Notas: | GANRY, J. Breeding banana and plantain for resistance to disease and pests. Montpellier, France: CIRAD-FLHOR, 1992. Proceeding of the International Symposium on Genetic Improvement of Banana for Resistance...
Conteúdo: | Isolated nuclei from leaf tissue of Musa balbisiana and M. acuminata were stained with propidium iodide and nuclear DNA contents (2C) were measured by flowcytometry. To determine nuclear DNA content in absolute units, fluorescence intensiy of Musa nuclei were compared with fluorescence intensity of nuclei isolated from Glycine max with Known nuclear genome size. Generally, the 2C DNA contents of diploid Musa clones were lower in comparison with other angiosperms. Nuclear DNA size among Musa balbisiana (2C =1.14pg) was significantly lower than DNA content of M. acuminata subspecies and diploid cultivar pisang Mas (1.23-1.26pg). The flow-cytometry has potential applications in Musa taxonomy, cross-breeding and biotechnology. Two DNA-based marker techniqueswere used for genomic characterization of different species, cultivars, and a mutant clone of the genus Musa. Hybridization in-gel of Hinf I-digested genomic DNA with the P-labelled synthetic oligonucleotides (GATA)4, (GTG)5, and (CA)8 revealed differences between clones of AA, AAA, AAAA, AAB, ABB, and BB genotypes. Thus, oligonucleotide fingerprinting can be applied for taxonomic studies of clones and the indentification of duplications among accession of Musa germplasm  collections (both in vivo and in vitro). Random DNA fragments were amplified by the polymerase chain reaction (PCR) using single or pair-wise combinations of a series of short oligonucleotide primers...
Palavras-Chave: | Congresso; Plantano.
Thesagro: | Banana; Fungo; Musa sp; Vírus.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (10138-1) - EMB - LV - -- - 634.7720601 - G198b -  - DIS