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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
04/03/2016 |
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Data da última atualização: |
04/03/2016 |
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Autoria: |
LEMOS, V. F.; GUARANÁ, E. L. S.; AFONSO, J. A. B.; FAGLIARI, J. J.; SILVA, P. C.; SOARES P. C.; MENDONÇA, C. L. de. |
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Afiliação: |
VÂNIA F. LEMOS, UFRPE; EDUARDO L. S. GUARANÁ, UFRPE; JOSÉ A. B. AFONSO, UFRPE; JOSÉ J. FAGLIARI, UNESP; PAULO C. SILVA, UNESP; PIERRE C. SOARES, UFRPE; CARLA LOPES DE MENDONÇA, UFRPE. |
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Título: |
Effects of intramammary infection on whey proteinograms os sheep during lactation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 3, p. 230-236, mar. 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
The study aimed to identify potential biomarkers of mammary gland infection in Santa Inês sheep. Commercial flocks of sheep provided the same hygiene, sanitary, and nutritional management under semi-intensive production systems were monitored during the lactation stage-and assessed 15, 30, 60, and 90 days after delivery (through the end of lactation and weaning). The California Mastitis Test (CMT) was performed on the mammary glands. Milk was collected for bacterial examination and protein analysis. Bacterial culture and biochemical characterization of the samples were performed. Forty-two milk samples from healthy glands (negative CMT and bacterial testing) and 43 milk samples from infected glands (positive CMT and bacterial testing) taken at the predefined time points were assessed. A rennin solution was used to obtain the whey. The proteins analysis was performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which allowed for the quantification of nine whey proteins produced in healthy glands: serum albumin, lactoferrin, IgA, IgG heavy-chain (IgG HC), IgG light-chain (IgG LC), total IgG (IgG HC + IgG LC), ?-lactalbumin, ?-lactoglobulin, protein with MW 15.000 Da, protein with MW 29.000 Da and eleven whey proteins secreted by infected glands, including haptoglobin and ?-1-acid glycoprotein. A comparison of whey proteins between healthy and infected glands showed increases (P<0.05) in the secreted and total contents of all proteins, except for IgG LC and ?-lactoalbumin. The most significant changes were observed in ?-1- acid glycoprotein, lactoferrin and haptoglobin, which showed three-, five-, and seven-fold increases in secretion, respectively. This study showed that haptoglobin, ?-1-acid glycoprotein, lactoferrin, albumin, and the IgA and IgG immunoglobulins may serve as potential biomarkers for mammary gland infection in sheep. MenosThe study aimed to identify potential biomarkers of mammary gland infection in Santa Inês sheep. Commercial flocks of sheep provided the same hygiene, sanitary, and nutritional management under semi-intensive production systems were monitored during the lactation stage-and assessed 15, 30, 60, and 90 days after delivery (through the end of lactation and weaning). The California Mastitis Test (CMT) was performed on the mammary glands. Milk was collected for bacterial examination and protein analysis. Bacterial culture and biochemical characterization of the samples were performed. Forty-two milk samples from healthy glands (negative CMT and bacterial testing) and 43 milk samples from infected glands (positive CMT and bacterial testing) taken at the predefined time points were assessed. A rennin solution was used to obtain the whey. The proteins analysis was performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which allowed for the quantification of nine whey proteins produced in healthy glands: serum albumin, lactoferrin, IgA, IgG heavy-chain (IgG HC), IgG light-chain (IgG LC), total IgG (IgG HC + IgG LC), ?-lactalbumin, ?-lactoglobulin, protein with MW 15.000 Da, protein with MW 29.000 Da and eleven whey proteins secreted by infected glands, including haptoglobin and ?-1-acid glycoprotein. A comparison of whey proteins between healthy and infected glands showed increases (P<0.05) in the secreted and total contents of all proteins, except for ... Mostrar Tudo |
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Palavras-Chave: |
Marcador biológico; Mastite subclínica; Proteína de fase aguda; Subclinical mastitis. |
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Thesagro: |
Leite; Ovelha. |
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Thesaurus Nal: |
Acute phase proteins; Biomarkers; Ewes; Milk. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140638/1/Effects-of-intramammary-infection.pdf
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Marc: |
LEADER 02780naa a2200313 a 4500
001 2039647
005 2016-03-04
008 2015 bl uuuu u00u1 u #d
100 1 $aLEMOS, V. F.
245 $aEffects of intramammary infection on whey proteinograms os sheep during lactation.
260 $c2015
520 $aThe study aimed to identify potential biomarkers of mammary gland infection in Santa Inês sheep. Commercial flocks of sheep provided the same hygiene, sanitary, and nutritional management under semi-intensive production systems were monitored during the lactation stage-and assessed 15, 30, 60, and 90 days after delivery (through the end of lactation and weaning). The California Mastitis Test (CMT) was performed on the mammary glands. Milk was collected for bacterial examination and protein analysis. Bacterial culture and biochemical characterization of the samples were performed. Forty-two milk samples from healthy glands (negative CMT and bacterial testing) and 43 milk samples from infected glands (positive CMT and bacterial testing) taken at the predefined time points were assessed. A rennin solution was used to obtain the whey. The proteins analysis was performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which allowed for the quantification of nine whey proteins produced in healthy glands: serum albumin, lactoferrin, IgA, IgG heavy-chain (IgG HC), IgG light-chain (IgG LC), total IgG (IgG HC + IgG LC), ?-lactalbumin, ?-lactoglobulin, protein with MW 15.000 Da, protein with MW 29.000 Da and eleven whey proteins secreted by infected glands, including haptoglobin and ?-1-acid glycoprotein. A comparison of whey proteins between healthy and infected glands showed increases (P<0.05) in the secreted and total contents of all proteins, except for IgG LC and ?-lactoalbumin. The most significant changes were observed in ?-1- acid glycoprotein, lactoferrin and haptoglobin, which showed three-, five-, and seven-fold increases in secretion, respectively. This study showed that haptoglobin, ?-1-acid glycoprotein, lactoferrin, albumin, and the IgA and IgG immunoglobulins may serve as potential biomarkers for mammary gland infection in sheep.
650 $aAcute phase proteins
650 $aBiomarkers
650 $aEwes
650 $aMilk
650 $aLeite
650 $aOvelha
653 $aMarcador biológico
653 $aMastite subclínica
653 $aProteína de fase aguda
653 $aSubclinical mastitis
700 1 $aGUARANÁ, E. L. S.
700 1 $aAFONSO, J. A. B.
700 1 $aFAGLIARI, J. J.
700 1 $aSILVA, P. C.
700 1 $aSOARES P. C.
700 1 $aMENDONÇA, C. L. de
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35, n. 3, p. 230-236, mar. 2015.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
27/11/2017 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BARRETO, C. K.; MAIA, A. L. R. S.; VEIGA, M. de O.; PRATES, J. F.; BRANDÃO, F. Z.; SOUZA-FABJAN, J. M. G.; FERREIRA, M. I. C.; FACO, O.; FONSECA, J. F. da. |
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Afiliação: |
Carla Knopp Barreto; Ana Lúcia Rosa Silva Maia; Maíra de Oliveira Veiga; Jader F. Prates; Felipe Zandonadi Brandão; Joanna Maria Gonçalves Souza-Fabjan; MARIA IZABEL CARNEIRO FERREIRA, CNPC; OLIVARDO FACO, CNPC; JEFERSON FERREIRA DA FONSECA, CNPC. |
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Título: |
Use of cloprostenol to synchronize estrus after induction by light program in anestrous dairy goats. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Revista Brasileira de Reprodução Animal, Belo Horizonte, v. 41, n. 1, p. 505, jan./mar. 2017. |
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Idioma: |
Inglês |
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Notas: |
Edição dos anais do XXII Congresso Brasileiro de Reprodução Animal (CBRA), Santos, SP, Brasil, maio 2017. |
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Conteúdo: |
This study aimed to test the possibility to synchronize estrus with cloprostenol in dairy goats submitted to light program, after estrous detection. Both estrous control techniques do not require milk discharge. The sequential association of these two tools can provide synchronous estrus during the non-breeding season, allowing AI to be performed in timed like scheme similar to the breeding season in dairy goats. [Uso de cloprostenol para sincronização do estro após indução por meio de programa de luz em caprinos leiteiros na estação de anestro estacional]. |
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Palavras-Chave: |
Caprino leiteiro; Dairy goat; Light program; Oestrus synchronization; Programa de luz; Taxa de prenhez. |
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Thesagro: |
Cabra leiteira; Caprino; Ciclo Estral; Sincronização do Cio. |
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Thesaurus NAL: |
Goats; pregnancy rate. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/167546/1/cnpc-2017-Use-of.pdf
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Marc: |
LEADER 01756nam a2200361 a 4500
001 2080796
005 2019-09-23
008 2017 bl uuuu u00u1 u #d
100 1 $aBARRETO, C. K.
245 $aUse of cloprostenol to synchronize estrus after induction by light program in anestrous dairy goats.$h[electronic resource]
260 $aRevista Brasileira de Reprodução Animal, Belo Horizonte, v. 41, n. 1, p. 505, jan./mar. 2017.$c2017
500 $aEdição dos anais do XXII Congresso Brasileiro de Reprodução Animal (CBRA), Santos, SP, Brasil, maio 2017.
520 $aThis study aimed to test the possibility to synchronize estrus with cloprostenol in dairy goats submitted to light program, after estrous detection. Both estrous control techniques do not require milk discharge. The sequential association of these two tools can provide synchronous estrus during the non-breeding season, allowing AI to be performed in timed like scheme similar to the breeding season in dairy goats. [Uso de cloprostenol para sincronização do estro após indução por meio de programa de luz em caprinos leiteiros na estação de anestro estacional].
650 $aGoats
650 $apregnancy rate
650 $aCabra leiteira
650 $aCaprino
650 $aCiclo Estral
650 $aSincronização do Cio
653 $aCaprino leiteiro
653 $aDairy goat
653 $aLight program
653 $aOestrus synchronization
653 $aPrograma de luz
653 $aTaxa de prenhez
700 1 $aMAIA, A. L. R. S.
700 1 $aVEIGA, M. de O.
700 1 $aPRATES, J. F.
700 1 $aBRANDÃO, F. Z.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aFERREIRA, M. I. C.
700 1 $aFACO, O.
700 1 $aFONSECA, J. F. da
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Embrapa Caprinos e Ovinos (CNPC) |
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