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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
23/11/2011 |
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Data da última atualização: |
30/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEREIRA, I. S.; SILVA, D. D. da; CASELA. C. R.; TARDIN, F. D.; ABREU, M. S. de. |
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Afiliação: |
IGOR SOUZA PEREIRA, IFPA; DAGMA DIONISIA DA SILVA, CNPMS; CARLOS ROBERTO CASELA, Pesquisador aposentado - CNPMS; FLAVIO DESSAUNE TARDIN, CNPMS; MARIO SOBRAL DE ABREU, UFLA. |
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Título: |
Resistência de linhagens genitoras e híbridos simples de sorgo a Colletotrichum sublineolum, agente causal da antracnose. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Revista Caatinga, Mossoró, v. 24, n. 2, p. 46-51, abr./jun. 2011. |
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Idioma: |
Português |
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Conteúdo: |
Foi avaliada a reação de resistência de dez híbrido s simples de sorgo (Sorghum bicolor) e 14 linhagens genitoras destes híbridos ao patógeno Colletotrichum sublineolum, agente causal da antracnose. Em casa de vegetação, os genótipos foram inoculados se paradamente com 20 isolados monospóricos obtidos de diferentes regiões produtoras do país. A avaliação foi realizada aos dez dias após a inoculação, utilizando-se escala de notas. Nenhum genótipo de sorgo foi resistente a todos os isolados inoculados. A linhagem CMSXS657 foi resistente a 95%; a linhagem ATF14, a 90%; ATF08, a 85% e CMSXS210, a 70% dos isolados. Dentre os híbridos, destacou-se o BRS305 (CMSX S210A X BR012R), resistente a 75% dos isolados; 9920045 (ATF14A X CMSXS180R), a 65% dos isolados; BRS308 (CMSXS233A X BR012R), a 60% dos isolados e BRS650 (CMSXS222A X CMSXS657R), a 55% dos isolados. As linhagens BR001 e CMSXS222 foram suscetíveis a 90% dos isolados. A frequência de resistência dos híbridos foi igual ou inferior às linhagens genitoras. Exceção faz-se ao híbrido BRS305 (CMSXS2 10A X BR012R), que foi resistente a um maior número de isolados que seus genitores. Nenhum dos isolados testados foi virulento a todos os genótipos. Os isolados de Campo Novo dos Parecis (MT) foram os mais virulentos enquanto os isolados de Jardinópolis (SP) foram os menos virulentos. |
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Thesagro: |
Antracnose; Doença de planta; Sorghum Bicolor; Sorgo. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/47489/1/Resistencia-linhagens.pdf
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Marc: |
LEADER 02052naa a2200217 a 4500
001 1906826
005 2017-05-30
008 2011 bl uuuu u00u1 u #d
100 1 $aPEREIRA, I. S.
245 $aResistência de linhagens genitoras e híbridos simples de sorgo a Colletotrichum sublineolum, agente causal da antracnose.$h[electronic resource]
260 $c2011
520 $aFoi avaliada a reação de resistência de dez híbrido s simples de sorgo (Sorghum bicolor) e 14 linhagens genitoras destes híbridos ao patógeno Colletotrichum sublineolum, agente causal da antracnose. Em casa de vegetação, os genótipos foram inoculados se paradamente com 20 isolados monospóricos obtidos de diferentes regiões produtoras do país. A avaliação foi realizada aos dez dias após a inoculação, utilizando-se escala de notas. Nenhum genótipo de sorgo foi resistente a todos os isolados inoculados. A linhagem CMSXS657 foi resistente a 95%; a linhagem ATF14, a 90%; ATF08, a 85% e CMSXS210, a 70% dos isolados. Dentre os híbridos, destacou-se o BRS305 (CMSX S210A X BR012R), resistente a 75% dos isolados; 9920045 (ATF14A X CMSXS180R), a 65% dos isolados; BRS308 (CMSXS233A X BR012R), a 60% dos isolados e BRS650 (CMSXS222A X CMSXS657R), a 55% dos isolados. As linhagens BR001 e CMSXS222 foram suscetíveis a 90% dos isolados. A frequência de resistência dos híbridos foi igual ou inferior às linhagens genitoras. Exceção faz-se ao híbrido BRS305 (CMSXS2 10A X BR012R), que foi resistente a um maior número de isolados que seus genitores. Nenhum dos isolados testados foi virulento a todos os genótipos. Os isolados de Campo Novo dos Parecis (MT) foram os mais virulentos enquanto os isolados de Jardinópolis (SP) foram os menos virulentos.
650 $aAntracnose
650 $aDoença de planta
650 $aSorghum Bicolor
650 $aSorgo
700 1 $aSILVA, D. D. da
700 1 $aCASELA. C. R.
700 1 $aTARDIN, F. D.
700 1 $aABREU, M. S. de
773 $tRevista Caatinga, Mossoró$gv. 24, n. 2, p. 46-51, abr./jun. 2011.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/03/2009 |
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Data da última atualização: |
19/06/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
JOSÉ, A. A. F. B. V.; GAMA, M. A. S.; LANNA, D. P. D. |
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Afiliação: |
A. A. F. B. V. José, ESALQ/USP; Marco Antonio Sundfeld da Gama, Embrapa Gado de Leite; Dante Pazzanese Duarte Lanna, ESALQ/USP. |
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Título: |
Effects of trans-10, cis-12 conjugated linoleic acid on gene expression and lipid metabolism of adipose tissue of growing pigs. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 7, n. 2, p. 284-294, 2008. |
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DOI: |
https://doi.org/10.4238/vol7-2gmr417 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of the present study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (CLA) in adipose tissue explant cultures of growing pigs on the following responses: lipogenesis (measured as rate of 14C-labeled glucose incorporation over a subsequent 2-h incubation in the presence or absence of insulin), lipolysis (release of non-esterified fatty acid over a 2-h incubation in the presence or absence of isoproterenol), activities of lipogenic enzymes, and mRNA abundance of fatty acid synthase (FAS). Adipose tissue explants from nine growing pigs (78 ± 3 kg) were cultured in 199 medium with insulin, dexamethasone and antibiotics for 4, 12, 24, and 48 h. The treatments were 1) control: 100 µM polyvinyl alcohol (PVA); 2) pGH: 100 ng/mL porcine growth hormone (pGH) plus 100 µM PVA; 3) CLA200: 200 µM trans-10, cis-12 CLA; 4) CLA50: 50 µM trans-10, cis-12 CLA, and 5) LA: 200 µM linoleic acid. Fatty acids were added along with PVA (2:1), respectively, for 24 h. Explants were collected after each culture period and assayed for lipogenesis. Transcripts of FAS mRNA were quantified by real-time RT-PCR after 24 and 48 h. Lipolysis and activities of FAS, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and NADP-malate dehydrogenase were determined after 48 h. As expected, glucose incorporation was decreased (P < 0.05) in response to pGH treatment (positive control). LA had no effect on any parameter evaluated. Treatment with trans-10, cis-12 CLA decreased FAS activity (P < 0.05), but NADPH-generating enzymes were unaffected by treatments. Consistent with reduction in FAS activity, both lipid synthesis and FAS mRNA abundance were reduced with chronic CLA treatment, pGH increased baseline and stimulated lipolysis (P < 0.05) after 48 h of culture, while CLA treatment had no effect on nonesterified fatty acid release. Results of this study showed that trans-10, cis-12 CLA alters lipogenesis but has no effect on lipolysis in cultures of pig adipose tissue. MenosThe objective of the present study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (CLA) in adipose tissue explant cultures of growing pigs on the following responses: lipogenesis (measured as rate of 14C-labeled glucose incorporation over a subsequent 2-h incubation in the presence or absence of insulin), lipolysis (release of non-esterified fatty acid over a 2-h incubation in the presence or absence of isoproterenol), activities of lipogenic enzymes, and mRNA abundance of fatty acid synthase (FAS). Adipose tissue explants from nine growing pigs (78 ± 3 kg) were cultured in 199 medium with insulin, dexamethasone and antibiotics for 4, 12, 24, and 48 h. The treatments were 1) control: 100 µM polyvinyl alcohol (PVA); 2) pGH: 100 ng/mL porcine growth hormone (pGH) plus 100 µM PVA; 3) CLA200: 200 µM trans-10, cis-12 CLA; 4) CLA50: 50 µM trans-10, cis-12 CLA, and 5) LA: 200 µM linoleic acid. Fatty acids were added along with PVA (2:1), respectively, for 24 h. Explants were collected after each culture period and assayed for lipogenesis. Transcripts of FAS mRNA were quantified by real-time RT-PCR after 24 and 48 h. Lipolysis and activities of FAS, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and NADP-malate dehydrogenase were determined after 48 h. As expected, glucose incorporation was decreased (P < 0.05) in response to pGH treatment (positive control). LA had no effect on any parameter evaluated. Treatment with trans-10, cis-12... Mostrar Tudo |
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Palavras-Chave: |
Adipose tissue explants; Bioactive fatty acid. |
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Thesaurus NAL: |
conjugated linoleic acid; lipogenesis; swine. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/596283/1/Effects-of-trans-10-cis-12-conjugated.pdf
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Marc: |
LEADER 02764naa a2200217 a 4500
001 1596283
005 2024-06-19
008 2008 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4238/vol7-2gmr417$2DOI
100 1 $aJOSÉ, A. A. F. B. V.
245 $aEffects of trans-10, cis-12 conjugated linoleic acid on gene expression and lipid metabolism of adipose tissue of growing pigs.$h[electronic resource]
260 $c2008
520 $aThe objective of the present study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (CLA) in adipose tissue explant cultures of growing pigs on the following responses: lipogenesis (measured as rate of 14C-labeled glucose incorporation over a subsequent 2-h incubation in the presence or absence of insulin), lipolysis (release of non-esterified fatty acid over a 2-h incubation in the presence or absence of isoproterenol), activities of lipogenic enzymes, and mRNA abundance of fatty acid synthase (FAS). Adipose tissue explants from nine growing pigs (78 ± 3 kg) were cultured in 199 medium with insulin, dexamethasone and antibiotics for 4, 12, 24, and 48 h. The treatments were 1) control: 100 µM polyvinyl alcohol (PVA); 2) pGH: 100 ng/mL porcine growth hormone (pGH) plus 100 µM PVA; 3) CLA200: 200 µM trans-10, cis-12 CLA; 4) CLA50: 50 µM trans-10, cis-12 CLA, and 5) LA: 200 µM linoleic acid. Fatty acids were added along with PVA (2:1), respectively, for 24 h. Explants were collected after each culture period and assayed for lipogenesis. Transcripts of FAS mRNA were quantified by real-time RT-PCR after 24 and 48 h. Lipolysis and activities of FAS, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and NADP-malate dehydrogenase were determined after 48 h. As expected, glucose incorporation was decreased (P < 0.05) in response to pGH treatment (positive control). LA had no effect on any parameter evaluated. Treatment with trans-10, cis-12 CLA decreased FAS activity (P < 0.05), but NADPH-generating enzymes were unaffected by treatments. Consistent with reduction in FAS activity, both lipid synthesis and FAS mRNA abundance were reduced with chronic CLA treatment, pGH increased baseline and stimulated lipolysis (P < 0.05) after 48 h of culture, while CLA treatment had no effect on nonesterified fatty acid release. Results of this study showed that trans-10, cis-12 CLA alters lipogenesis but has no effect on lipolysis in cultures of pig adipose tissue.
650 $aconjugated linoleic acid
650 $alipogenesis
650 $aswine
653 $aAdipose tissue explants
653 $aBioactive fatty acid
700 1 $aGAMA, M. A. S.
700 1 $aLANNA, D. P. D.
773 $tGenetics and Molecular Research$gv. 7, n. 2, p. 284-294, 2008.
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Embrapa Gado de Leite (CNPGL) |
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