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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
21/03/2011 |
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Data da última atualização: |
17/04/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MORALES, A. M. A. P.; BOREM, A.; LOUREIRO, M.; MOREIRA, R. S.; ABDELNOOR, R. V.; GRAHAM, M. A. |
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Afiliação: |
AGUIDA MARIA ALVES PEREIRA MORALES, UFV; ALUÍZIO BOREM; MARCELO LOUREIRO; RENATA STOLF MOREIRA; RICARDO VILELA ABDELNOOR, CNPSO; MICHELLE A. GRAHAM, USDA-ARS. |
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Título: |
Expression analyses of candidate resistance genes in the Rpp4 Asian Soybean Rust resistance locus. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
2010. |
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Idioma: |
Inglês |
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Notas: |
Edição de Poster da 11. Annual National Outreach Scholarship Conference, Raleigh. |
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Conteúdo: |
Asian Soybean Rust (ASR), caused Phakopsora pachyrhizi, is considered the most severe soybean disease around the world. Infection of susceptible genotypes leads to early defoliation, incomplete seed development, and yield losses as high as 80%. Five ASR resistance genes have been identified in soybean: Rpp1, Rpp2, Rpp3, Rpp4 and Rpp5. Of particular interest is Rpp4, which has remained stable and confers resistance against P. pachyrhizi isolates from around the world. Rpp4 was mapped to soybean linkage group G (chromosome 18), 1.9 cM from simple sequence repeat (SSR) marker Satt288. Sequencing of this region in the susceptible genotype Williams 82 identified a cluster of three CC-NBS-LRR resistance genes. Virus Induced Gene Silencing was used to demonstrate that orthologous genes were responsible for resistance. We have now sequenced a >460 kb region of the Rpp4 locus in the resistant mapping parent PI459025B. Eight CC-NBS-LRR resistance genes have been identified in this region. In order to obtain more information about Rpp4 function, we are using real time quantitative PCR (qRT-PCR) to analyze the expression of all eight genes in different plant tissues, in different stages of development and after inoculation with P. pachyrhizi. We have developed a single pair of primers from the NBD domain that monitors the expression of all eight genes. Direct sequencing of the RT-PCR product differentiates between the eight genes. Detailed sequence analyses of the Rpp4 locus suggest that intra- and intergenic duplications and recombination have played an important role in creating genetic diversity. Alternative splicing of intragenic duplications may create additional sequence diversity at an RNA level. We are developing primers that will allow us to monitor alternative splicing events. Sequencing of the RT-PCR products will determine if alternative splicing plays a role in generating additional sequence diversity at the Rpp4 locus. MenosAsian Soybean Rust (ASR), caused Phakopsora pachyrhizi, is considered the most severe soybean disease around the world. Infection of susceptible genotypes leads to early defoliation, incomplete seed development, and yield losses as high as 80%. Five ASR resistance genes have been identified in soybean: Rpp1, Rpp2, Rpp3, Rpp4 and Rpp5. Of particular interest is Rpp4, which has remained stable and confers resistance against P. pachyrhizi isolates from around the world. Rpp4 was mapped to soybean linkage group G (chromosome 18), 1.9 cM from simple sequence repeat (SSR) marker Satt288. Sequencing of this region in the susceptible genotype Williams 82 identified a cluster of three CC-NBS-LRR resistance genes. Virus Induced Gene Silencing was used to demonstrate that orthologous genes were responsible for resistance. We have now sequenced a >460 kb region of the Rpp4 locus in the resistant mapping parent PI459025B. Eight CC-NBS-LRR resistance genes have been identified in this region. In order to obtain more information about Rpp4 function, we are using real time quantitative PCR (qRT-PCR) to analyze the expression of all eight genes in different plant tissues, in different stages of development and after inoculation with P. pachyrhizi. We have developed a single pair of primers from the NBD domain that monitors the expression of all eight genes. Direct sequencing of the RT-PCR product differentiates between the eight genes. Detailed sequence analyses of the Rpp4 locus suggest tha... Mostrar Tudo |
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Palavras-Chave: |
Ferrugem asiática da soja. |
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Thesagro: |
Doença fungica; Fungo. |
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Thesaurus Nal: |
Disease resistance; Fungal diseases of plants; Soybean rust. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/30153/1/abdelnoor.conf..pdf
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Marc: |
LEADER 02727nam a2200253 a 4500
001 1881697
005 2018-04-17
008 2010 bl uuuu u00u1 u #d
100 1 $aMORALES, A. M. A. P.
245 $aExpression analyses of candidate resistance genes in the Rpp4 Asian Soybean Rust resistance locus.
260 $a2010.$c2010
500 $aEdição de Poster da 11. Annual National Outreach Scholarship Conference, Raleigh.
520 $aAsian Soybean Rust (ASR), caused Phakopsora pachyrhizi, is considered the most severe soybean disease around the world. Infection of susceptible genotypes leads to early defoliation, incomplete seed development, and yield losses as high as 80%. Five ASR resistance genes have been identified in soybean: Rpp1, Rpp2, Rpp3, Rpp4 and Rpp5. Of particular interest is Rpp4, which has remained stable and confers resistance against P. pachyrhizi isolates from around the world. Rpp4 was mapped to soybean linkage group G (chromosome 18), 1.9 cM from simple sequence repeat (SSR) marker Satt288. Sequencing of this region in the susceptible genotype Williams 82 identified a cluster of three CC-NBS-LRR resistance genes. Virus Induced Gene Silencing was used to demonstrate that orthologous genes were responsible for resistance. We have now sequenced a >460 kb region of the Rpp4 locus in the resistant mapping parent PI459025B. Eight CC-NBS-LRR resistance genes have been identified in this region. In order to obtain more information about Rpp4 function, we are using real time quantitative PCR (qRT-PCR) to analyze the expression of all eight genes in different plant tissues, in different stages of development and after inoculation with P. pachyrhizi. We have developed a single pair of primers from the NBD domain that monitors the expression of all eight genes. Direct sequencing of the RT-PCR product differentiates between the eight genes. Detailed sequence analyses of the Rpp4 locus suggest that intra- and intergenic duplications and recombination have played an important role in creating genetic diversity. Alternative splicing of intragenic duplications may create additional sequence diversity at an RNA level. We are developing primers that will allow us to monitor alternative splicing events. Sequencing of the RT-PCR products will determine if alternative splicing plays a role in generating additional sequence diversity at the Rpp4 locus.
650 $aDisease resistance
650 $aFungal diseases of plants
650 $aSoybean rust
650 $aDoença fungica
650 $aFungo
653 $aFerrugem asiática da soja
700 1 $aBOREM, A.
700 1 $aLOUREIRO, M.
700 1 $aMOREIRA, R. S.
700 1 $aABDELNOOR, R. V.
700 1 $aGRAHAM, M. A.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 363 | |
| 61. |  | POLIZEL-PODANOSQUI, A. M.; PEREIRA, R. M.; STOLF-MOREIRA, R.; MARCELINO-GUIMARÃES, F. C.; ABDELNOOR, R. V. Identificação de genes diferencialmente expressos em soja em resposta à Phakopsora pachyrhizi pela metodologia ACP. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: anais. Brasília, DF: Embrapa, 2012. 4 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 62. | | MORALES, A.; O'ROURKE, J. A.; BORÉM, A.; ABDELNOOR, R. V.; PEDLEY, K. F.; WHITHAM, S. A.; GRAHAM, M. A. Combining transcriptome analyses and virus induced gene silencing to identify genes in the Rpp4-mediated asian soybean rust resistance Pathway. In: MOLECULAR & CELLULAR BIOLOGY OF TE SOYBEAN, 14., Des Moines, 2012. [Proceedings...]. Des Moines: Iowa State University, 2012. p. 137.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 63. | | SCHUSTER, I.; ABDELNOOR, R. V.; MARTINS, M. K.; CARVALHO, V. P.; DIAS, W. P.; BARROS, E. G.; MOREIRA, M. A. Heranca da resistencia a raca 4+ do nematoide de cisto da soja (Heterodera glycines Ichinohe) no cruzamento Hartwig x PI437654. Genetics and Molecular Biology, Ribeirao Preto, v.23, n.3, p.453-454, Sept. 2000. Supplement. Trabalho apresentado no 46, Congresso Nacional de Genetica, 19 a 23 de setembro de 2000, Aguas de Lindoia, SP.| Biblioteca(s): Embrapa Soja. |
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| 64. | | ABDELNOOR, R. V.; DIAS, W. P.; MARIN, S. R. R.; CARVALHO, V. P.; MARTINS, M. K.; ALMEIDA, A. M. R. Diversidade genetica entre isolados do nematoide de cisto da soja (Heterodera glycines) avaliada com o uso de marcadores RAPD. Genetics and Molecular Biology, Ribeirao Preto, v. 22, n. 3, p. 329, Oct. 1999. Supplement. Trabalho apresentado no 45o. Congresso Nacional de Genética, 1999, Gramado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 66. | | CAMARGO, P. C.; CATELLI, L. L.; RINCÃO, M. P.; PIOVEZANI, A. R.; ARIAS, C. A. A.; ABDELNOOR, R. V. Mapeamento do locus de resistência à ferrugem asiática no genótipo PI 561356. In: CONGRESSO BRASILEIRO DE SOJA, 5.; MERCOSOJA 2009, Goiânia. Anais... Londrina: Embrapa Soja, 2009. Seção trabalhos, t. 171. 1 CD-ROM. Editado por Adilson de Oliveira Júnior, Odilon Ferreira Saraiva, Clara Beatriz Hoffmann Campo, César de Castro.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 67. | | CATELLI, L. L.; ARIAS, C. A. A.; CAMARGO, P. O.; MARIN, S. R. R.; ABDELNOOR, R. V. Mapeamento molecular do gene de resistência à ferrugem asiática nos genótipos PI 200487 (Kinoshita) e Shiranui. Fitopatologia Brasileira, Brasília, DF, v. 32, p. S325, ago. 2007. Suplemento, resumo 1084. Edição dos Resumos do XL Congresso Brasileiro de Fitopatologia, Maringá, PR, ago. 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Soja. |
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| 70. | | CARVALHO, V. de P.; ABDELNOOR, R. V.; ALMEIDA, A. M. R.; ARIAS, C. A. A.; KIIHL, R. A. de S. Molecular markers linked to the resistance to race 3 of the soybean cyst nematode Heterodera glycines. Crop Breeding and Applied Biotechnology, Londrina, v. 2, n. 1, p. 77-84, mar. 2002.| Biblioteca(s): Embrapa Soja. |
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| 71. | | RINCÃO, M. P; CRUZ, B. G.; YOKOYAMA, A.; KUWAHARA, M. K.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. Perfil transcricional de genes essenciais ao fungo phakopsora pachyrhizi causador da ferrugem asiática da soja. In: CONGRESSO BRASILEIRO DE SOJA, 7.; MERCOSOJA, 2015, Florianópolis. Tecnologia e mercado global: perspectivas para soja: anais. Londrina: Embrapa Soja, 2015. 3 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 73. | | CORRÊA, R. X.; ABDELNOOR, R. V.; FALEIRO, F. G.; CRUZ, C. D.; MOREIRA, M. A.; BARROS, E. G. de. Genetic distances in soybean based on RAPD markers. Bragantia, Campinas, v. 58, n.1, p. 15-22, 1999.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 74. | | DIAS, W. P.; SILVA, J. F. V.; KIIHL, R. A. de S.; HIROMOTO, D. M.; ABDELNOOR, R. V. Quebra da resistência da cv. Hartwig por população de campo do nematóide de cisto da soja (Heterodera glycines). Pesquisa Agropecuária Brasileira, Brasília, v. 33, n.6, p. 971-974 , jun. 1998.| Biblioteca(s): Embrapa Soja; Embrapa Unidades Centrais. |
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| 75. | | CAMOLESE, A. C.; BOTELHO, L.; PINHEIRO, M.; FRAGA, T. R.; MAIA, M. S.; RINCÃO, M. P.; ABDELNOOR, R. V. Ranqueamento de populações de soja quanto à resistência a Phakopsora pachyrhizi. JORNADA ACADÊMICA DA EMBRAPA SOJA, 7., 2012, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2012. p. 158-165. (Embrapa Soja. Documentos, 333). Editado por Paula Gerón Saiz de Mello. Disponível em: .| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 76. | | YAMANAKA, N.; LEMOS, N. G.; UNO, M.; AKAMATSU, H.; YAMAOKA, Y.; ABDELNOOR, R. V.; BRACCINI, A. L. e; SUENAGA, K. Resistance to Asian Soybean Rust in soybean lines with the pyramided three Rpp genes. Crop Breeding and Applied Biotechnology, Viçosa, v. 13, n. 1, p. 75-82, Mar. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 79. | | CUNHA, M. J.; SANTANA, R. H.; VERAS, H. C. T; ABDELNOOR, R. V.; ALMEIDA, J. D.; BARROS, L. M. G. Validação de um gene de Glycine max preferencialmente expresso em folha. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. p. 73.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 80. | | VERAS, H. C. T.; SANTANA, R. H.; ALMEIDA, J. D. de; ABDELNOOR, R. V.; BARROS, L. M. G. Validação de um gene de soja (Glycine max) preferencialmente expresso em semente. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. Resumo 040.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 363 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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