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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
17/12/2015 |
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Data da última atualização: |
05/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
KUMA, K. M.; LOPES-CAITAR, V. S.; ROMERO, C. C. T.; SILVA, S. M. H.; CARVALHO, M. C. C. G.; ABDELNOOR, R. V.; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C. |
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Afiliação: |
UEL; UEL; CNPSo; UEL; UENP; RICARDO VILELA ABDELNOOR, CNPSO; WALDIR PEREIRA DIAS, CNPSO; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO. |
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Título: |
A high efficient protocol for soybean root transformation by Agrobacterium rhizogenes and most stable reference genes for RT-qPCR analysis. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plant Cell Reports, v. 34, n. 11, p. 1987-2000, 2015. |
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ISSN: |
1432-203X |
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DOI: |
10.1007/s00299-015-1845-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Gene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis in composite plants, mainly under nematode infection. MenosGene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis i... Mostrar Tudo |
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Thesagro: |
Soja. |
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Categoria do assunto: |
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Marc: |
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100 1 $aKUMA, K. M.
245 $aA high efficient protocol for soybean root transformation by Agrobacterium rhizogenes and most stable reference genes for RT-qPCR analysis.$h[electronic resource]
260 $c2015
520 $aGene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis in composite plants, mainly under nematode infection.
650 $aSoja
700 1 $aLOPES-CAITAR, V. S.
700 1 $aROMERO, C. C. T.
700 1 $aSILVA, S. M. H.
700 1 $aCARVALHO, M. C. C. G.
700 1 $aABDELNOOR, R. V.
700 1 $aDIAS, W. P.
700 1 $aMARCELINO-GUIMARÃES, F. C.
773 $tPlant Cell Reports$gv. 34, n. 11, p. 1987-2000, 2015.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 363 | |
| 5. |  | ABDELNOOR, R. V.; MORIYAMA, H.; MACKENZIE, S. Identification of Msh-1-homologue genes in soybean, rice, tomato and common bean. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 255. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.| Biblioteca(s): Embrapa Soja. |
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| 13. | | YAMANAKA, N.; HERCULANO, S. F. H.; ABDELNOOR, R. V.; NEPOMUCENO, A. L.; YORINORI, J. T. Avaliação de resistência a SDS utilizando isolado brasileiro em genótipos de soja chineses e japoneses resistentes a isolado argentino. In: CONGRESSO BRASILEIRO DE SOJA, 4., 2006, Londrina. Resumos... Londrina: Embrapa Soja, 2006. p. 104. Organizado por Odilon Ferreira Saraiva, Simone Ery Grosskopf.| Biblioteca(s): Embrapa Soja. |
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| 14. | | CARDOSO, R.; CAITAR, V. L.; FERREIRA, E. C.; MARCELINO-GUIMARÃES, F. C.; ABDELNOOR, R. V. Associative mapping for bacterial pustule in soybean (Mapeamento associativo para pústula bacteriana em soja) in: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 51., 2019, Recife. Anais... Brasília, DF: SBF, 2019. p. 156.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 15. | | MORALES, A. M. A. P.; BORÉM, A.; GRAHAM, M.; ABDELNOOR, R. V. Advances on molecular studies of the interaction soybean - Asian rust. Crop Breeding Applied Biotechnology, Viçosa, MG, v. 12, n. 1, p. 1-7, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 16. | | SANTANA, R. H.; VERAS, H. C. T.; ABDELNOOR, R. V.; BARROS, L. M. G. Análise experimental da expressão diferencial de um transcrito de soja identificado através de northern eletrônico. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. Resumo 011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | SILVA, J. F. V.; ABDELNOOR, R. V.; COSTA, C. L.; BAÍA, G. S. Análise de RAPD em isolados de Fusarium spp. parasitas de ovos de Heterodera glycines ou de plantas de soja. Fitopatologia Brasileira, Brasilia, DF, v. 25, n. 3, p. 491-496, set. 2000.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Soja. |
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| 18. | | LEMOS, N. G.; BRACCINI, A. de L.; ABDELNOOR, R. V.; SUENAGA, K.; YAMANAKA, N. Análise da resistência genética de linhagens de soja contendo genes de resistência para a ferrugem asiática piramidados. In: CONGRESO DE LA SOJA DEL MERCOSUR, 5.; FORO DE LA SOJA ASIA, 1., 2011, Rosário. Un grano: un universo. [Rosário: Asociación de la Cadena de la Soja Argentina], 2011. 4 p. 1 CD-ROM. MERCOSOJA 2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 19. | | CORRÊA, R. X.; BARROS, E. G.; ABDELNOOR, R. V.; MOREIRA, M. A. Intra-specific genetic map of soybean based on RAPD markers. In: CONGRESSO NACIONAL DE GENÉTICA, 41., 1995, Caxambu. Programa e resumos... Revista Brasileira de Genética, v. 18, n. 3, p. 162, set. 1995. Suplemento. A.153.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 20. | | LEMOS, N. G.; BRACCINI, A. de L.; ABDELNOOR, R. V.; YAMANAKA, N. Influence of three resistance genes, RPP2, RPP4 and RPP5 on resistance characters against asian soybean rust. In: WORLD SOYBEAN RESEARCH CONFERENCE, 8., 2009, Beijing. Developing a global soy blueprint for a safe secure and sustainable supply: abstracts. Beijing: Chinese Academy of Agricultural Sciences: Institute of Crop Science, 2009. p. 132, ref. P321. WSRC 2009. Editado por Lijuan Qiu, Rongxia Guan, Jian Jin, Qijan Song, Shuntang Guo, Wenbin Li, Yuanchao Wang, Tianfu Han, Xiaobing Liu, Deyue Yu, Lianzhou Jiang, Deliang Peng.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 363 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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