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Registro Completo |
Biblioteca(s): |
Embrapa Meio-Norte; Embrapa Uva e Vinho. |
Data corrente: |
01/09/1992 |
Data da última atualização: |
30/08/2019 |
Autoria: |
REUNIÃO SUL-AMERICANA DE BOTÂNICA, 1., 1938, Rio de Janeiro. |
Título: |
Anais... |
Ano de publicação: |
1938 |
Fonte/Imprenta: |
Rio de Janeiro: MA-Jardim Botanico do Rio de Janeiro, 1938. |
Volume: |
v.1 |
Páginas: |
341p. |
Idioma: |
Português |
Conteúdo: |
v. 1. Introdução; v. 2. Botânica sistemática (criptógamos) |
Palavras-Chave: |
congresso. |
Thesagro: |
Botânica; Taxonomia. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00505nam a2200169 a 4500 001 1534930 005 2019-08-30 008 1938 bl uuuu u00u1 u #d 100 1 $aREUNIÃO SUL-AMERICANA DE BOTÂNICA, 1., 1938, Rio de Janeiro. 245 $aAnais... 260 $aRio de Janeiro: MA-Jardim Botanico do Rio de Janeiro$c1938 300 $a341p. v.1 490 $vv.1 520 $av. 1. Introdução; v. 2. Botânica sistemática (criptógamos) 650 $aBotânica 650 $aTaxonomia 653 $acongresso
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Embrapa Uva e Vinho (CNPUV) |
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Registro Completo
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
06/05/2019 |
Data da última atualização: |
06/05/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
COELHO, S. V. B.; ROSA, S. D. V. F. da; FANTAZZINI, T. B.; BAUTE, J. L.; SILVA, L. C. |
Afiliação: |
Stefânia Vilas Boas Coelho, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; Tatiana Botelho Fantazzini, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; Júlia Lima Baute, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; Luciano Coutinho Silva, Departamento de Biologia Celular e Molecular/Universidade Federal da Paraíba - UFPB. |
Título: |
Cryopreservation in Coffea canephora Pierre seeds: slow and fast cooling. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Ciência e Agrotecnologia, v. 42, n. 6, p. 588-597, Nov/Dec. 2018 |
Idioma: |
Português |
Notas: |
Título em português Criopreservação em sementes de Coffea canephora Pierre: Resfriamento lento e rápido. |
Conteúdo: |
Coffee is one of the main agricultural commodities in the country, and it is important to conservation of plant material for breeding programs. Cryopreservation is a promising alternative for preserving in the long-term the germplasm of species considered recalcitrant. However, studies should be performed to achieve maximum survival of seedlings after immersion in liquid nitrogen. The objective of this work was to find a cryopreservation protocol for storing seeds of Coffea canephora, studying two methods of cryopreservation, slow and fast cooling. Seeds were subjected to drying in silica gel up to the water content of 0.25 g g-1. In the first experiment, dried seeds were subjected to treatments of slow cooling at speeds of -1 ºC min-1,-3 ºC min-1 and -5 ºC min-1 until the end temperatures of -40 ºC, -50 ºC and -60 ºC, by means of a bio freezer and subsequently immersed in liquid nitrogen. In the second experiment, the best result was selected of the first experiment and compared with the rapid cooling, in which dried seeds, with 0.25 g g-1 of water content, were immersed directly into liquid nitrogen. Physiological and biochemical alterations occurring in the seeds after cryopreservation were evaluated. Coffea canephora seeds respond better to cryopreservation by rapid cooling, when compared to slow cooling. Drying, one of the cryopreservation steps does not affect the viability of Coffea canephora Pierre seeds, when these seeds are dried to 0.25 g g-1 of water content. Catalase and esterase enzymes are good biochemical markers for cryopreserved coffee seeds and their activity is greater in larger seed physiological quality. MenosCoffee is one of the main agricultural commodities in the country, and it is important to conservation of plant material for breeding programs. Cryopreservation is a promising alternative for preserving in the long-term the germplasm of species considered recalcitrant. However, studies should be performed to achieve maximum survival of seedlings after immersion in liquid nitrogen. The objective of this work was to find a cryopreservation protocol for storing seeds of Coffea canephora, studying two methods of cryopreservation, slow and fast cooling. Seeds were subjected to drying in silica gel up to the water content of 0.25 g g-1. In the first experiment, dried seeds were subjected to treatments of slow cooling at speeds of -1 ºC min-1,-3 ºC min-1 and -5 ºC min-1 until the end temperatures of -40 ºC, -50 ºC and -60 ºC, by means of a bio freezer and subsequently immersed in liquid nitrogen. In the second experiment, the best result was selected of the first experiment and compared with the rapid cooling, in which dried seeds, with 0.25 g g-1 of water content, were immersed directly into liquid nitrogen. Physiological and biochemical alterations occurring in the seeds after cryopreservation were evaluated. Coffea canephora seeds respond better to cryopreservation by rapid cooling, when compared to slow cooling. Drying, one of the cryopreservation steps does not affect the viability of Coffea canephora Pierre seeds, when these seeds are dried to 0.25 g g-1 of water content. Cat... Mostrar Tudo |
Palavras-Chave: |
Cristal de gelo; Drying in silica gel; Ice crystals; Isoenzymes; Physiological quality; Qualidade fisiológica; Secagem em sílica gel; Semente recalcitrante. |
Thesagro: |
Isoenzima; Secagem. |
Thesaurus NAL: |
Recalcitrant seeds. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/196979/1/Cryopreservation-in-Coffea-canephora-Pierre-seeds.pdf
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Marc: |
LEADER 02666naa a2200313 a 4500 001 2108759 005 2019-05-06 008 2018 bl uuuu u00u1 u #d 100 1 $aCOELHO, S. V. B. 245 $aCryopreservation in Coffea canephora Pierre seeds$bslow and fast cooling.$h[electronic resource] 260 $c2018 500 $aTítulo em português Criopreservação em sementes de Coffea canephora Pierre: Resfriamento lento e rápido. 520 $aCoffee is one of the main agricultural commodities in the country, and it is important to conservation of plant material for breeding programs. Cryopreservation is a promising alternative for preserving in the long-term the germplasm of species considered recalcitrant. However, studies should be performed to achieve maximum survival of seedlings after immersion in liquid nitrogen. The objective of this work was to find a cryopreservation protocol for storing seeds of Coffea canephora, studying two methods of cryopreservation, slow and fast cooling. Seeds were subjected to drying in silica gel up to the water content of 0.25 g g-1. In the first experiment, dried seeds were subjected to treatments of slow cooling at speeds of -1 ºC min-1,-3 ºC min-1 and -5 ºC min-1 until the end temperatures of -40 ºC, -50 ºC and -60 ºC, by means of a bio freezer and subsequently immersed in liquid nitrogen. In the second experiment, the best result was selected of the first experiment and compared with the rapid cooling, in which dried seeds, with 0.25 g g-1 of water content, were immersed directly into liquid nitrogen. Physiological and biochemical alterations occurring in the seeds after cryopreservation were evaluated. Coffea canephora seeds respond better to cryopreservation by rapid cooling, when compared to slow cooling. Drying, one of the cryopreservation steps does not affect the viability of Coffea canephora Pierre seeds, when these seeds are dried to 0.25 g g-1 of water content. Catalase and esterase enzymes are good biochemical markers for cryopreserved coffee seeds and their activity is greater in larger seed physiological quality. 650 $aRecalcitrant seeds 650 $aIsoenzima 650 $aSecagem 653 $aCristal de gelo 653 $aDrying in silica gel 653 $aIce crystals 653 $aIsoenzymes 653 $aPhysiological quality 653 $aQualidade fisiológica 653 $aSecagem em sílica gel 653 $aSemente recalcitrante 700 1 $aROSA, S. D. V. F. da 700 1 $aFANTAZZINI, T. B. 700 1 $aBAUTE, J. L. 700 1 $aSILVA, L. C. 773 $tCiência e Agrotecnologia$gv. 42, n. 6, p. 588-597, Nov/Dec. 2018
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