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Registro Completo |
Biblioteca(s): |
Embrapa Agroenergia; Embrapa Soja. |
Data corrente: |
02/10/2015 |
Data da última atualização: |
13/11/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; NAKAYAMA, T. J.; REIS, R. R.; FARIAS, J. R. B.; HARMON, F. G.; MOLINARI, H. B. C.; MOLINARI, M. D. C.; NEPOMUCENO, A. L. |
Afiliação: |
UEL; AUTOR; AUTOR; UFV; UEL; JOSE RENATO BOUCAS FARIAS, CNPSO; U C BERKELEY - BERKELEY; HUGO BRUNO CORREA MOLINARI, CNPAE; UEL; ALEXANDRE LIMA NEPOMUCENO, CNPSO. |
Título: |
Transcriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Plos One, v. 10, n. 9, e0139051, 2015. |
Páginas: |
16 p. |
DOI: |
10.1371/journal.pone.0139051 |
Idioma: |
Inglês |
Conteúdo: |
The soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day. MenosThe soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean ge... Mostrar Tudo |
Palavras-Chave: |
Drought stress; Soybean. |
Thesagro: |
Estiagem; Resistência à seca; Seca; Soja. |
Thesaurus Nal: |
Drought tolerance; Soybeans; Transcriptome. |
Categoria do assunto: |
-- F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166647/1/nepomuceno-plos-one.pdf
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Marc: |
LEADER 02592naa a2200361 a 4500 001 2079599 005 2017-11-13 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1371/journal.pone.0139051$2DOI 100 1 $aMARCOLINO-GOMES, J. 245 $aTranscriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day.$h[electronic resource] 260 $c2015 300 $a16 p. 520 $aThe soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day. 650 $aDrought tolerance 650 $aSoybeans 650 $aTranscriptome 650 $aEstiagem 650 $aResistência à seca 650 $aSeca 650 $aSoja 653 $aDrought stress 653 $aSoybean 700 1 $aRODRIGUES, F. A. 700 1 $aFUGANTI-PAGLIARINI, R. 700 1 $aNAKAYAMA, T. J. 700 1 $aREIS, R. R. 700 1 $aFARIAS, J. R. B. 700 1 $aHARMON, F. G. 700 1 $aMOLINARI, H. B. C. 700 1 $aMOLINARI, M. D. C. 700 1 $aNEPOMUCENO, A. L. 773 $tPlos One$gv. 10, n. 9, e0139051, 2015.
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Embrapa Soja (CNPSO) |
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