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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
07/04/2004 |
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Data da última atualização: |
11/09/2013 |
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Autoria: |
LEMOS, N. G.; YAMAGUCHI-SHINOZAKI, K.; NAKASHIMA, K.; MOLINA, J. C.; STOLF, R.; MORALES, A. M. R.; LUGLE, S. M.; SILVEIRA, C. A. de; MARIN, S. R. R.; BINNECK, E.; FARIAS, J. R. B.; NEUMAIER, N.; NEPOMUCENO, A. L. |
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Título: |
Introduction of genes that confer drought stress tolerance in soybean [Glycine max (L.) Merril] by biobalistic. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
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Páginas: |
p. 256. |
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Série: |
(Embrapa Soja. Documentos, 228). |
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Idioma: |
Inglês |
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Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. |
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Conteúdo: |
The soybean crop moves the Brazilian economy from the primary sector to the industry, generating many direct and indirect jobs. However, yearly billions of dolars are lost due abiotic and biotic stresses occurring during the crop cycle. Abiotic stresses, precisely drought, is one of the main causes of yield losses. Aiming to minimize this damage, scientists are using biotechnology methods, introducing genes in plants to improve tolerance to drought stress. The plant drought response is regulated by a complex of genes generating a variety of tolerance mecanisms. The complexity of such responses make difficult of using classic breeding methods in the development tolerant varieties. However, genetic engineering of key genes in key metabolic pathways has succeded in incresing drought tolerance in many crops. Our objective was to introduce in soybean the expression cassets pRD29:DREB1 and pE35S:P5CS-129A, using an Embrapa's patent method of Biobalistics that confer dehydration tolerance in plants. DREB1A (Dehidration Responsive Biding Element Protein) is a transcription factor responsible by the activation of genes involved in the drought, freezing and saline stress response; P5CS-129A is also a key enzime in the pathway for proline production. Proline increasing in the cytosol has been related to the cell turgor preservation and protection of cell structures during dehydration by scavenging oxidative radicals. The transformed plants have been generated and tested by PCR. More than 2590 embryos were transformed until now. Nineteen plants were PCR positives for the AHAS gene and none PCR positive plants were detected until now for the p35S:P5CS-129A construct. Number of insertion copies and expression levels will be tested in the positive plants. Physiological characterization in drought conditions will be carried on in promissing events. MenosThe soybean crop moves the Brazilian economy from the primary sector to the industry, generating many direct and indirect jobs. However, yearly billions of dolars are lost due abiotic and biotic stresses occurring during the crop cycle. Abiotic stresses, precisely drought, is one of the main causes of yield losses. Aiming to minimize this damage, scientists are using biotechnology methods, introducing genes in plants to improve tolerance to drought stress. The plant drought response is regulated by a complex of genes generating a variety of tolerance mecanisms. The complexity of such responses make difficult of using classic breeding methods in the development tolerant varieties. However, genetic engineering of key genes in key metabolic pathways has succeded in incresing drought tolerance in many crops. Our objective was to introduce in soybean the expression cassets pRD29:DREB1 and pE35S:P5CS-129A, using an Embrapa's patent method of Biobalistics that confer dehydration tolerance in plants. DREB1A (Dehidration Responsive Biding Element Protein) is a transcription factor responsible by the activation of genes involved in the drought, freezing and saline stress response; P5CS-129A is also a key enzime in the pathway for proline production. Proline increasing in the cytosol has been related to the cell turgor preservation and protection of cell structures during dehydration by scavenging oxidative radicals. The transformed plants have been generated and tested by PCR. More th... Mostrar Tudo |
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Thesagro: |
Soja. |
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Categoria do assunto: |
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Marc: |
LEADER 03105naa a2200313 a 4500
001 1466831
005 2013-09-11
008 2004 bl uuuu u00u1 u #d
100 1 $aLEMOS, N. G.
245 $aIntroduction of genes that confer drought stress tolerance in soybean [Glycine max (L.) Merril] by biobalistic.
260 $c2004
300 $ap. 256.
490 $a(Embrapa Soja. Documentos, 228).
500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
520 $aThe soybean crop moves the Brazilian economy from the primary sector to the industry, generating many direct and indirect jobs. However, yearly billions of dolars are lost due abiotic and biotic stresses occurring during the crop cycle. Abiotic stresses, precisely drought, is one of the main causes of yield losses. Aiming to minimize this damage, scientists are using biotechnology methods, introducing genes in plants to improve tolerance to drought stress. The plant drought response is regulated by a complex of genes generating a variety of tolerance mecanisms. The complexity of such responses make difficult of using classic breeding methods in the development tolerant varieties. However, genetic engineering of key genes in key metabolic pathways has succeded in incresing drought tolerance in many crops. Our objective was to introduce in soybean the expression cassets pRD29:DREB1 and pE35S:P5CS-129A, using an Embrapa's patent method of Biobalistics that confer dehydration tolerance in plants. DREB1A (Dehidration Responsive Biding Element Protein) is a transcription factor responsible by the activation of genes involved in the drought, freezing and saline stress response; P5CS-129A is also a key enzime in the pathway for proline production. Proline increasing in the cytosol has been related to the cell turgor preservation and protection of cell structures during dehydration by scavenging oxidative radicals. The transformed plants have been generated and tested by PCR. More than 2590 embryos were transformed until now. Nineteen plants were PCR positives for the AHAS gene and none PCR positive plants were detected until now for the p35S:P5CS-129A construct. Number of insertion copies and expression levels will be tested in the positive plants. Physiological characterization in drought conditions will be carried on in promissing events.
650 $aSoja
700 1 $aYAMAGUCHI-SHINOZAKI, K.
700 1 $aNAKASHIMA, K.
700 1 $aMOLINA, J. C.
700 1 $aSTOLF, R.
700 1 $aMORALES, A. M. R.
700 1 $aLUGLE, S. M.
700 1 $aSILVEIRA, C. A. de
700 1 $aMARIN, S. R. R.
700 1 $aBINNECK, E.
700 1 $aFARIAS, J. R. B.
700 1 $aNEUMAIER, N.
700 1 $aNEPOMUCENO, A. L.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 14,689 | |
| 161. |  | SERRONI, M. A. L. de O.; FUHRMANN, M. B.; AVILA, M. R. de; CARLI, R. de; OLIVEIRA, A. C. B. de. Avaliação de genótipos de soja na safra 2013/14. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 23.; ENCONTRO DE PÓS-GRADUAÇÃO, 16., 2014, Pelotas. [Anais.]. Pelotas: UFPel, 2014.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 166. | | VERNETTI JUNIOR, F. de J.; PARFITT, J. M. B.; AGUILA, L. S. H. D. Avaliação de cultivares de soja irrigadas por aspersão em rotação com arroz irrigado. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: anais. Brasília, DF: Embrapa; Londrina: Embrapa Soja, 2012. 1 CD-ROM. Editado por Adilson de Oliveira Júnior, Clara Beatriz Hoffmann-Campo, Regina Maria Villas Bôas de Campos Leite, Ricardo Vilela Abdelnoor. VI CBSoja.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 172. | | VIEIRA, S. S.; LOURENÇÃO, A. L.; GRAÇA, J. P. da; JANEGITZ, T.; SALVADOR, M. C.; OLIVEIRA, M. C. N. de; HOFFMANN-CAMPO, C. B. Biological aspects of Bemisia tabaci biotype B and the chemical causes of resistance in soybean genotypes. Arthropod-Plant Interactions, v. 10, n. 6, p. 525-534, Dec. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 173. | | VILLELA, A. T.; ANTUNES, I. F.; EICHHOLZ, C. J.; FEIJÓ, C. T.; BEVILAQUA, G. A. P.; GREHS, R. C. Biodlversity score and its effects on common bean (Phaseolus vulgaris L.) crop genetic diversity. Annual Report of the Bean Improvement Cooperative, Prosser, WA, n. 57, p. 259-260, March 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 174. | | ANDRADE, K.; BUENO, A. de F.; SILVA, D. M. da; STECCA, C. dos S.; PASINI, A.; OLIVEIRA, M. C. N. de. Bioecological characteristics of Chrysodeixis includens (Lepidoptera: Noctuidae) fed on different hosts. Austral Entomology, v. 55, n. 4, p. 449-454, nov. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 176. | | ALMEIDA, A. M. R.; SAKAI, J.; OLIVEIRA, T. G. O.; BELINTANI, P.; KITAJIMA, E. W.; SOUTO, E. R.; NOVAES, T. G. de; NORA, P. S. Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil. Fitopatologia Brasileira, Brasília, v. 30, n. 4, p. 366-373, July/Aug. 2005.| Biblioteca(s): Embrapa Soja. |
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| 177. | | BRIGHENTI, A. M.; VOLL, E.; GAZZIERO, D. L. P. Biology and management of Cardiospermum haliacabum. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 143. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 14,689 | |
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