| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Soja. |
|
Data corrente: |
07/04/2004 |
|
Data da última atualização: |
11/09/2013 |
|
Autoria: |
LEMOS, N. G.; YAMAGUCHI-SHINOZAKI, K.; NAKASHIMA, K.; MOLINA, J. C.; STOLF, R.; MORALES, A. M. R.; LUGLE, S. M.; SILVEIRA, C. A. de; MARIN, S. R. R.; BINNECK, E.; FARIAS, J. R. B.; NEUMAIER, N.; NEPOMUCENO, A. L. |
|
Título: |
Introduction of genes that confer drought stress tolerance in soybean [Glycine max (L.) Merril] by biobalistic. |
|
Ano de publicação: |
2004 |
|
Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
|
Páginas: |
p. 256. |
|
Série: |
(Embrapa Soja. Documentos, 228). |
|
Idioma: |
Inglês |
|
Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. |
|
Conteúdo: |
The soybean crop moves the Brazilian economy from the primary sector to the industry, generating many direct and indirect jobs. However, yearly billions of dolars are lost due abiotic and biotic stresses occurring during the crop cycle. Abiotic stresses, precisely drought, is one of the main causes of yield losses. Aiming to minimize this damage, scientists are using biotechnology methods, introducing genes in plants to improve tolerance to drought stress. The plant drought response is regulated by a complex of genes generating a variety of tolerance mecanisms. The complexity of such responses make difficult of using classic breeding methods in the development tolerant varieties. However, genetic engineering of key genes in key metabolic pathways has succeded in incresing drought tolerance in many crops. Our objective was to introduce in soybean the expression cassets pRD29:DREB1 and pE35S:P5CS-129A, using an Embrapa's patent method of Biobalistics that confer dehydration tolerance in plants. DREB1A (Dehidration Responsive Biding Element Protein) is a transcription factor responsible by the activation of genes involved in the drought, freezing and saline stress response; P5CS-129A is also a key enzime in the pathway for proline production. Proline increasing in the cytosol has been related to the cell turgor preservation and protection of cell structures during dehydration by scavenging oxidative radicals. The transformed plants have been generated and tested by PCR. More than 2590 embryos were transformed until now. Nineteen plants were PCR positives for the AHAS gene and none PCR positive plants were detected until now for the p35S:P5CS-129A construct. Number of insertion copies and expression levels will be tested in the positive plants. Physiological characterization in drought conditions will be carried on in promissing events. MenosThe soybean crop moves the Brazilian economy from the primary sector to the industry, generating many direct and indirect jobs. However, yearly billions of dolars are lost due abiotic and biotic stresses occurring during the crop cycle. Abiotic stresses, precisely drought, is one of the main causes of yield losses. Aiming to minimize this damage, scientists are using biotechnology methods, introducing genes in plants to improve tolerance to drought stress. The plant drought response is regulated by a complex of genes generating a variety of tolerance mecanisms. The complexity of such responses make difficult of using classic breeding methods in the development tolerant varieties. However, genetic engineering of key genes in key metabolic pathways has succeded in incresing drought tolerance in many crops. Our objective was to introduce in soybean the expression cassets pRD29:DREB1 and pE35S:P5CS-129A, using an Embrapa's patent method of Biobalistics that confer dehydration tolerance in plants. DREB1A (Dehidration Responsive Biding Element Protein) is a transcription factor responsible by the activation of genes involved in the drought, freezing and saline stress response; P5CS-129A is also a key enzime in the pathway for proline production. Proline increasing in the cytosol has been related to the cell turgor preservation and protection of cell structures during dehydration by scavenging oxidative radicals. The transformed plants have been generated and tested by PCR. More th... Mostrar Tudo |
|
Thesagro: |
Soja. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 03105naa a2200313 a 4500
001 1466831
005 2013-09-11
008 2004 bl uuuu u00u1 u #d
100 1 $aLEMOS, N. G.
245 $aIntroduction of genes that confer drought stress tolerance in soybean [Glycine max (L.) Merril] by biobalistic.
260 $c2004
300 $ap. 256.
490 $a(Embrapa Soja. Documentos, 228).
500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
520 $aThe soybean crop moves the Brazilian economy from the primary sector to the industry, generating many direct and indirect jobs. However, yearly billions of dolars are lost due abiotic and biotic stresses occurring during the crop cycle. Abiotic stresses, precisely drought, is one of the main causes of yield losses. Aiming to minimize this damage, scientists are using biotechnology methods, introducing genes in plants to improve tolerance to drought stress. The plant drought response is regulated by a complex of genes generating a variety of tolerance mecanisms. The complexity of such responses make difficult of using classic breeding methods in the development tolerant varieties. However, genetic engineering of key genes in key metabolic pathways has succeded in incresing drought tolerance in many crops. Our objective was to introduce in soybean the expression cassets pRD29:DREB1 and pE35S:P5CS-129A, using an Embrapa's patent method of Biobalistics that confer dehydration tolerance in plants. DREB1A (Dehidration Responsive Biding Element Protein) is a transcription factor responsible by the activation of genes involved in the drought, freezing and saline stress response; P5CS-129A is also a key enzime in the pathway for proline production. Proline increasing in the cytosol has been related to the cell turgor preservation and protection of cell structures during dehydration by scavenging oxidative radicals. The transformed plants have been generated and tested by PCR. More than 2590 embryos were transformed until now. Nineteen plants were PCR positives for the AHAS gene and none PCR positive plants were detected until now for the p35S:P5CS-129A construct. Number of insertion copies and expression levels will be tested in the positive plants. Physiological characterization in drought conditions will be carried on in promissing events.
650 $aSoja
700 1 $aYAMAGUCHI-SHINOZAKI, K.
700 1 $aNAKASHIMA, K.
700 1 $aMOLINA, J. C.
700 1 $aSTOLF, R.
700 1 $aMORALES, A. M. R.
700 1 $aLUGLE, S. M.
700 1 $aSILVEIRA, C. A. de
700 1 $aMARIN, S. R. R.
700 1 $aBINNECK, E.
700 1 $aFARIAS, J. R. B.
700 1 $aNEUMAIER, N.
700 1 $aNEPOMUCENO, A. L.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Soja (CNPSO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
12/11/2014 |
|
Data da última atualização: |
05/04/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 1 |
|
Autoria: |
SALLES, H. O.; BRAGA, A. C. L.; NASCIMENTO, M. T. dos S. C. do; SOUSA, A. M. P.; LIMA, A. R.; VIEIRA, L. da S.; CAVALCANTE, A. C. R.; EGITO, A. S. do; ANDRADE, L. B. da S. |
|
Afiliação: |
HEVILA OLIVEIRA SALLES, CNPC; Ana Carolina Linhares Braga, Graduação - Instituto Superior de Teologia Aplicada (INTA) - Sobral, CE, Brasil; Maria Thayana dos Santos Canuto do Nascimento, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; Ana Márjory Paiva Sousa, UVA - Sobral, CE, Brasil; Adriano Rodrigues Lima, CNPC; LUIZ DA SILVA VIEIRA, CNPC; ANTONIO CEZAR ROCHA CAVALCANTE, CNPC; ANTONIO SILVIO DO EGITO, CNPC; Lúcia Betânia da Silva Andrade, UVA - Sobral, CE, Brasil. |
|
Título: |
Lectin, hemolysin and protease inhibitors in seed fractions with ovicidal activity against Haemonchus contortus. |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 23, n. 2, p. 136-143, abr.-jun. 2014 |
|
DOI: |
http://dx.doi.org/10.1590/S1984-29612014050 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: Bioactive molecules of plant species are promising alternatives for the chemical control of gastrointestinal nematodes in ruminants. Extracts of native and exotic seed species from Brazil's semi-arid region were tested in vitro in an egg hatch assay and the bioactivity of their proteins was investigated. Each seed species was subjected to three extractions with three types of solvents. All the seeds showed ovicidal activity, which varied according to the solvents. Higher ovicidal activity was found in the molecule fractions of low molecular weight (<12 kDa) for Albizia lebbeck, Ipomoea asarifolia, Jatropha curcas, Libidibia ferrea, Moringa oleifera and Ricinus communis (P<0.05, Bonferroni test). The two fractions of Crotalaria spectabilis showed the same ovicidal activity (P>0.05, Bonferroni test). Hemagglutinating activity was detected in the fractions of C. spectabilis and M. oleifera fractions, hemolysin activity in the A. lebbeck and M. oleifera fractions, serine protease inhibitory activity in the A. lebbeck, I. asarifolia, J. curcas, M. oleifera and R. communis fractions, cysteine protease inhibitor activity in the M. oleifera fraction, and no protein activity in the L. ferrea fraction. The results of this work reveal new plant species with a potential for use in controlling nematode parasites in goats, thus opening a new field of research involving plant protein molecules with ovicidal properties. [Lectina, hemolisina e inibidores de protease em frações de sementes com atividade ovicida contra Haemonchus contortus]. Resumo: Moléculas bioativas de espécies vegetais são alternativas promissoras ao controle químico dos nematoides gastrintestinais em ruminantes. Extratos de sementes de espécies nativas e exóticas do Semiárido Brasileiro foram testados in vitro em ensaio de eclosão de ovos e investigada a natureza proteica da bioatividade. Três extrações com três solventes foram feitas para cada semente estudada. Todas as sementes apresentaram atividade ovicida, variando com o solvente utilizado. Maior taxa de inibição da eclosão concentrou-se nas frações de moléculas de baixa massa molecular (<12 kDa) para Albizia lebbeck, Ipomoea asarifolia, Jatropha curcas, Libidibia ferrea, Moringa oleifera e Ricinus communis (P<0,05, teste de Bonferroni). Crotalaria spectabilis mostrou atividade nas duas frações, sem diferença entre elas (P>0,05, teste de Bonferroni). Observou-se atividade hemaglutinante nas frações de C. spectabilis e M. oleifera, de hemolisina em A. lebbeck e M. oleifera, de atividade inibidora de protease da serina em A. lebbeck, I. asarifolia, J. curcas, M. oleifera e R. communis, de atividade inibidora de protease da cisteína em M. oleifera e nenhuma atividade proteica na fração de L. ferrea. Os resultados revelaram novas espécies botânicas com potencial de controle de nematoides em caprinos e um novo campo de pesquisa, o estudo de moléculas de origem proteica com atividade ovicida. MenosAbstract: Bioactive molecules of plant species are promising alternatives for the chemical control of gastrointestinal nematodes in ruminants. Extracts of native and exotic seed species from Brazil's semi-arid region were tested in vitro in an egg hatch assay and the bioactivity of their proteins was investigated. Each seed species was subjected to three extractions with three types of solvents. All the seeds showed ovicidal activity, which varied according to the solvents. Higher ovicidal activity was found in the molecule fractions of low molecular weight (<12 kDa) for Albizia lebbeck, Ipomoea asarifolia, Jatropha curcas, Libidibia ferrea, Moringa oleifera and Ricinus communis (P<0.05, Bonferroni test). The two fractions of Crotalaria spectabilis showed the same ovicidal activity (P>0.05, Bonferroni test). Hemagglutinating activity was detected in the fractions of C. spectabilis and M. oleifera fractions, hemolysin activity in the A. lebbeck and M. oleifera fractions, serine protease inhibitory activity in the A. lebbeck, I. asarifolia, J. curcas, M. oleifera and R. communis fractions, cysteine protease inhibitor activity in the M. oleifera fraction, and no protein activity in the L. ferrea fraction. The results of this work reveal new plant species with a potential for use in controlling nematode parasites in goats, thus opening a new field of research involving plant protein molecules with ovicidal properties. [Lectina, hemolisina e inibidores de protease em frações de s... Mostrar Tudo |
|
Palavras-Chave: |
Anthelmintic; Atividade ovicida; Nematódeo gastrintestinal. |
|
Thesagro: |
Anti-helmíntico; Caprino; Fitoterapia; Haemonchus Contortus; Helminto gastrintestinal; Lectina; Nematóide; Óleo essencial; Planta medicinal; Semente. |
|
Thesaurus NAL: |
Goats; Lectins; Phytotherapy; Plant extracts. |
|
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/111475/1/ap-Lectin.pdf
|
|
Marc: |
LEADER 04319naa a2200433 a 4500
001 1999880
005 2019-04-05
008 2014 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1590/S1984-29612014050$2DOI
100 1 $aSALLES, H. O.
245 $aLectin, hemolysin and protease inhibitors in seed fractions with ovicidal activity against Haemonchus contortus.$h[electronic resource]
260 $c2014
520 $aAbstract: Bioactive molecules of plant species are promising alternatives for the chemical control of gastrointestinal nematodes in ruminants. Extracts of native and exotic seed species from Brazil's semi-arid region were tested in vitro in an egg hatch assay and the bioactivity of their proteins was investigated. Each seed species was subjected to three extractions with three types of solvents. All the seeds showed ovicidal activity, which varied according to the solvents. Higher ovicidal activity was found in the molecule fractions of low molecular weight (<12 kDa) for Albizia lebbeck, Ipomoea asarifolia, Jatropha curcas, Libidibia ferrea, Moringa oleifera and Ricinus communis (P<0.05, Bonferroni test). The two fractions of Crotalaria spectabilis showed the same ovicidal activity (P>0.05, Bonferroni test). Hemagglutinating activity was detected in the fractions of C. spectabilis and M. oleifera fractions, hemolysin activity in the A. lebbeck and M. oleifera fractions, serine protease inhibitory activity in the A. lebbeck, I. asarifolia, J. curcas, M. oleifera and R. communis fractions, cysteine protease inhibitor activity in the M. oleifera fraction, and no protein activity in the L. ferrea fraction. The results of this work reveal new plant species with a potential for use in controlling nematode parasites in goats, thus opening a new field of research involving plant protein molecules with ovicidal properties. [Lectina, hemolisina e inibidores de protease em frações de sementes com atividade ovicida contra Haemonchus contortus]. Resumo: Moléculas bioativas de espécies vegetais são alternativas promissoras ao controle químico dos nematoides gastrintestinais em ruminantes. Extratos de sementes de espécies nativas e exóticas do Semiárido Brasileiro foram testados in vitro em ensaio de eclosão de ovos e investigada a natureza proteica da bioatividade. Três extrações com três solventes foram feitas para cada semente estudada. Todas as sementes apresentaram atividade ovicida, variando com o solvente utilizado. Maior taxa de inibição da eclosão concentrou-se nas frações de moléculas de baixa massa molecular (<12 kDa) para Albizia lebbeck, Ipomoea asarifolia, Jatropha curcas, Libidibia ferrea, Moringa oleifera e Ricinus communis (P<0,05, teste de Bonferroni). Crotalaria spectabilis mostrou atividade nas duas frações, sem diferença entre elas (P>0,05, teste de Bonferroni). Observou-se atividade hemaglutinante nas frações de C. spectabilis e M. oleifera, de hemolisina em A. lebbeck e M. oleifera, de atividade inibidora de protease da serina em A. lebbeck, I. asarifolia, J. curcas, M. oleifera e R. communis, de atividade inibidora de protease da cisteína em M. oleifera e nenhuma atividade proteica na fração de L. ferrea. Os resultados revelaram novas espécies botânicas com potencial de controle de nematoides em caprinos e um novo campo de pesquisa, o estudo de moléculas de origem proteica com atividade ovicida.
650 $aGoats
650 $aLectins
650 $aPhytotherapy
650 $aPlant extracts
650 $aAnti-helmíntico
650 $aCaprino
650 $aFitoterapia
650 $aHaemonchus Contortus
650 $aHelminto gastrintestinal
650 $aLectina
650 $aNematóide
650 $aÓleo essencial
650 $aPlanta medicinal
650 $aSemente
653 $aAnthelmintic
653 $aAtividade ovicida
653 $aNematódeo gastrintestinal
700 1 $aBRAGA, A. C. L.
700 1 $aNASCIMENTO, M. T. dos S. C. do
700 1 $aSOUSA, A. M. P.
700 1 $aLIMA, A. R.
700 1 $aVIEIRA, L. da S.
700 1 $aCAVALCANTE, A. C. R.
700 1 $aEGITO, A. S. do
700 1 $aANDRADE, L. B. da S.
773 $tRevista Brasileira de Parasitologia Veterinária, Jaboticabal$gv. 23, n. 2, p. 136-143, abr.-jun. 2014
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
