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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
01/12/1992 |
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Data da última atualização: |
01/12/1992 |
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Autoria: |
CATSIMPOOLAS, N.; ROGERS, D. A.; MEYER, E. W. |
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Título: |
Immunochemical and disc electrophoresis study of soybean trypsin inhibitor SBTIA-2. |
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Ano de publicação: |
1969 |
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Fonte/Imprenta: |
Cereal Chemistry, v.46, p.136-144, mar. 1969. |
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Descrição Física: |
il. |
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Idioma: |
Inglês |
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Conteúdo: |
The immunochemical properties of the soybean trypsin inhibitor SBTIA-2 were studied by double gel diffusion, immunoelectrophoresis, single immunodiffusion, and complement fixation with the use of an antisoybean water-extract serum 106. As little as 0.03 microgram of the inhibitor can be detected by the micro complement fixation method with 400-fold dilution of antiserum. The inhibitor was found to be immunochemically identical with a corresponding protein in the total soybean water extract, and thus it is not an artifact of isolation. Stoichiometric addition of trypsin greatly reduces the antigenic sites of the inhibitor as examined by complement fixation. The single immunodiffusion technique can be used for quantitative estimation of inhibitor in the range of 0.02 to 1.00 mg. of the compound. By disc electrophoresis, impurities present in the commercial preparation of the inhibitor exhibit distinctly different patterns in presence and absence of persulfate catalyst, and in gels of various polyacrylamide concentrations. Microdensitometer tracings of the stained bands showed that the inhibitor contained the following amounts of apparent impurities in gels polymerized as indicated: 4% gels, ammonium persulfate, none; 7% riboflavin and light, 5%; 7%, ammonium persulfate, 43%; and 11%, riboflavin and light, 22%. The possibility of artifact formation caused by the persulfate catalyst is discussed. |
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Palavras-Chave: |
Disc electrophoresis; Disco; Imuno-difusao radial; Imunoeletroforese; Inibidor de tripsina; Radial immunodiffusion; SBTIA-2; Soybean; Trypsin inhibitor. |
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Thesagro: |
Eletroforese; Soja. |
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Thesaurus Nal: |
immunoelectrophoresis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02225naa a2200301 a 4500
001 1457479
005 1992-12-01
008 1969 bl --- 0-- u #d
100 1 $aCATSIMPOOLAS, N.
245 $aImmunochemical and disc electrophoresis study of soybean trypsin inhibitor SBTIA-2.
260 $c1969
300 $cil.
520 $aThe immunochemical properties of the soybean trypsin inhibitor SBTIA-2 were studied by double gel diffusion, immunoelectrophoresis, single immunodiffusion, and complement fixation with the use of an antisoybean water-extract serum 106. As little as 0.03 microgram of the inhibitor can be detected by the micro complement fixation method with 400-fold dilution of antiserum. The inhibitor was found to be immunochemically identical with a corresponding protein in the total soybean water extract, and thus it is not an artifact of isolation. Stoichiometric addition of trypsin greatly reduces the antigenic sites of the inhibitor as examined by complement fixation. The single immunodiffusion technique can be used for quantitative estimation of inhibitor in the range of 0.02 to 1.00 mg. of the compound. By disc electrophoresis, impurities present in the commercial preparation of the inhibitor exhibit distinctly different patterns in presence and absence of persulfate catalyst, and in gels of various polyacrylamide concentrations. Microdensitometer tracings of the stained bands showed that the inhibitor contained the following amounts of apparent impurities in gels polymerized as indicated: 4% gels, ammonium persulfate, none; 7% riboflavin and light, 5%; 7%, ammonium persulfate, 43%; and 11%, riboflavin and light, 22%. The possibility of artifact formation caused by the persulfate catalyst is discussed.
650 $aimmunoelectrophoresis
650 $aEletroforese
650 $aSoja
653 $aDisc electrophoresis
653 $aDisco
653 $aImuno-difusao radial
653 $aImunoeletroforese
653 $aInibidor de tripsina
653 $aRadial immunodiffusion
653 $aSBTIA-2
653 $aSoybean
653 $aTrypsin inhibitor
700 1 $aROGERS, D. A.
700 1 $aMEYER, E. W.
773 $tCereal Chemistry$gv.46, p.136-144, mar. 1969.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 14.690 | |
| 14621. |  | GARCIA, A. Semeadura: cultivares, épocas, densidade, tratamento de sementes e inoculação. In: PUIGNAU, J. P. (Ed.). Produccion de soja. Montevideo: IICA-PROCISUR, 1992. p. 85-107. (IICA-PROCISUR. Dialogo, 34). Trabajos presentados a los Cursos sobre Produccion de Soja realizados en Santa Cruz, Bolivia: 1-6/8/88; Encarnacion, Paraguay: 28/8-1/9/89; M. Juarez, Cordoba, Argentina: 27/31-8/90.| Biblioteca(s): Embrapa Soja. |
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| 14623. | | BORGES, M.; FERREIRA, L. T.; COSTA, M. L. M. da; SCARPATI, M. V.; SANTOS, H. M. dos; LOURENCO, R. T. Semioquimicos associados aos parasitoides, Trissolcus basalis e Telenomus podisi, para controle dos percevejos, Nezara viridula e Euschistus heros, pragas da soja. Brasilia: EMBRAPA-CENARGEN, 1997 6p. (EMBRAPA-CENARGEN. Comunicado Tecnico, 18)| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14627. | | PURCINO, H. M. A.; BRACELOS, A. O.; VERZIGNASSI, J. R.; AROEIRA, L. J.; FERNANDES, C. D.; PACIULLO, D. S. C. Utilização e contribuição de leguminosas na produção animal. Informe Agropecuário, Belo Horizonte, v. 26, n. 226, p. 76-96, 2005. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 14640. | | GAZZIERO, D. L. P.; KARAM, D.; VOLL, E. Control de malezas. In: EMBRAPA. Centro Nacional de Pesquisa de Soja (Londrina, PR). El cultivo de la soja en los tropicos: mejoramiento y produccion. Roma: FAO, 1995. p.123-130. (Coleccion FAO: Produccion y Proteccion Vegetal, 27).| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 14.690 | |
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