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Biblioteca(s): |
Embrapa Agropecuária Oeste. |
Data corrente: |
14/11/2014 |
Data da última atualização: |
14/11/2014 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
RIBEIRO, L. M.; PRATA, J. J. T. M.; CECCON, G. |
Afiliação: |
LUAN MARLON RIBEIRO, GRADUANDO EM AGRONOMIA – UNIGRAN; JORGE JUNIOR THEODORO MARTINS PRATA, GRADUANDO EM AGRONOMIA – FACULDADES ANHANGUERA DE DOURADOS; GESSI CECCON, CPAO. |
Título: |
Germinação e vigor de sementes de braquiária, submetidas aos diferentes niveis de água. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO À PESQUISA DA EMBRAPA, 2014, Dourados. Resumos... Brasília, DF: Embrapa, 2014. 1 CD-ROM; JIPE 2014. |
ISBN: |
978-85-7035-366-5 |
Idioma: |
Português |
Thesagro: |
Semente. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/111703/1/43-germinacao-luan.pdf
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Marc: |
LEADER 00555nam a2200145 a 4500 001 2000156 005 2014-11-14 008 2014 bl uuuu u00u1 u #d 020 $a978-85-7035-366-5 100 1 $aRIBEIRO, L. M. 245 $aGerminação e vigor de sementes de braquiária, submetidas aos diferentes niveis de água.$h[electronic resource] 260 $aIn: JORNADA DE INICIAÇÃO À PESQUISA DA EMBRAPA, 2014, Dourados. Resumos... Brasília, DF: Embrapa, 2014. 1 CD-ROM; JIPE 2014.$c2014 650 $aSemente 700 1 $aPRATA, J. J. T. M. 700 1 $aCECCON, G.
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Embrapa Agropecuária Oeste (CPAO) |
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Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
10/08/2018 |
Data da última atualização: |
24/09/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
NICIURA, S. C. M.; CRUVINEL, G. G.; MORAES, C. V.; DONATONI, F. A. B.; MALAGO JUNIOR, W.; BENAVIDES, M. V.; CHAGAS, A. C. de S. |
Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; Giovanna Gabrielle Cruvinel, UNICEP; Caroline Valério Moraes, UFSCar; FLAVIA ALINE BRESSANI DONATONI, CPPSE; WILSON MALAGO JUNIOR, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
Título: |
PCR-based genotyping of SNP markers in sheep. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Molecular Biology Reports, v.45, n.4, p.651-654, aug. 2018. |
ISSN: |
1573-4978 |
DOI: |
https://doi.org/10.1007/s11033-018-4206-8 |
Idioma: |
Inglês |
Conteúdo: |
Single nucleotide polymorphisms (SNPs) are the main type of variation in genome, enabling them to be associated with traits of economic importance in livestock. Genome-wide association studies (GWAS) have led to the discovery of SNPs associated with desirable traits in sheep. However, in these studies, SNPs are genotyped by high-throughput methods in genome scale, which are expensive and require sophisticated equipment and analysis methods. Therefore, the goal of this study was to develop a reliable, rapid, and inexpensive polymerase chain reaction (PCR)-based method to genotype a medium number of animals for a few candidate SNPs previously associated with desirable phenotypes in sheep by GWAS, using markers associated with gastrointestinal nematode resistance as a model. DNA extracted from white-blood cells of 150 sheep was submitted to PCR amplification followed by agarose gel electrophoresis and determination of banding pattern. Tetra-primer ARMS-PCR was successfully optimized after changes in annealing temperature; annealing and extension times; concentration of MgCl2 and DNA; ratios of inner, outer, forward and reverse primer; and addition of adjuvants, for genotyping the OAR2_14765360, OAR6_81718546, OAR11_62887032, and OAR12_69606944 SNPs in sheep. An extensive optimization of tetra-primer ARMS-PCR resulted in a suitable, simple, cost-effective PCR-based method of genotyping four SNP markers previously detected by chip arrays. |
Palavras-Chave: |
PCR RFLP; Resistência nematódeo gastrintestinal; Tetra primer ARMS PCR. |
Thesagro: |
Marcador Molecular; Ovino. |
Thesaurus NAL: |
Genome. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02300naa a2200289 a 4500 001 2094070 005 2018-09-24 008 2018 bl uuuu u00u1 u #d 022 $a1573-4978 024 7 $ahttps://doi.org/10.1007/s11033-018-4206-8$2DOI 100 1 $aNICIURA, S. C. M. 245 $aPCR-based genotyping of SNP markers in sheep.$h[electronic resource] 260 $c2018 520 $aSingle nucleotide polymorphisms (SNPs) are the main type of variation in genome, enabling them to be associated with traits of economic importance in livestock. Genome-wide association studies (GWAS) have led to the discovery of SNPs associated with desirable traits in sheep. However, in these studies, SNPs are genotyped by high-throughput methods in genome scale, which are expensive and require sophisticated equipment and analysis methods. Therefore, the goal of this study was to develop a reliable, rapid, and inexpensive polymerase chain reaction (PCR)-based method to genotype a medium number of animals for a few candidate SNPs previously associated with desirable phenotypes in sheep by GWAS, using markers associated with gastrointestinal nematode resistance as a model. DNA extracted from white-blood cells of 150 sheep was submitted to PCR amplification followed by agarose gel electrophoresis and determination of banding pattern. Tetra-primer ARMS-PCR was successfully optimized after changes in annealing temperature; annealing and extension times; concentration of MgCl2 and DNA; ratios of inner, outer, forward and reverse primer; and addition of adjuvants, for genotyping the OAR2_14765360, OAR6_81718546, OAR11_62887032, and OAR12_69606944 SNPs in sheep. An extensive optimization of tetra-primer ARMS-PCR resulted in a suitable, simple, cost-effective PCR-based method of genotyping four SNP markers previously detected by chip arrays. 650 $aGenome 650 $aMarcador Molecular 650 $aOvino 653 $aPCR RFLP 653 $aResistência nematódeo gastrintestinal 653 $aTetra primer ARMS PCR 700 1 $aCRUVINEL, G. G. 700 1 $aMORAES, C. V. 700 1 $aDONATONI, F. A. B. 700 1 $aMALAGO JUNIOR, W. 700 1 $aBENAVIDES, M. V. 700 1 $aCHAGAS, A. C. de S. 773 $tMolecular Biology Reports$gv.45, n.4, p.651-654, aug. 2018.
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