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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Hortaliças. |
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Data corrente: |
12/05/1997 |
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Data da última atualização: |
12/05/1997 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FARIA, J. C.; SOUZA-DIAS, J.A.C.; SLACK, S. A.; MAXWELL, D. P. |
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Afiliação: |
EMBRAPA-CNPAF, Goiania, GO. |
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Título: |
A new geminivirus associated with tomato in the State of Sao Paulo, Brazil. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Plant Disease, v.81, n.4, p.423, 1997. |
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Idioma: |
Inglês
Português |
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Notas: |
Notes. |
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Conteúdo: |
The apical growth of about 20% of young tomato plants in observed fields near Campinas, State of São Paulo, Brazil, had yellow streaking of veins. Leaf symptoms developed into patches of yellow mosaic and the leaves became wavy. The whitefly Bemisia tabaci Genn. transmitted a pathogen from the infected tomato plants to healthy tomato and potato plants, reproducing the original symptoms in tomato. The apical leaves of infected potatoes showed yellow or green mottle that developed into leaf distortion with yellow blotches, symptoms indistinguishable from potato-deforming mosaic disease (2). DNA was extracted from these tomato and potato plants (1). Using DNA from the infected tomato plant, polymerase chain reaction (PCR) with the degenerate primer pair PAC1v1978/ PAV1c715 (1), which amplifies part of the rep gene (AC1 ORF), the common region (CR), and part of the cp gene (AV1 ORF), and with the primer pair PBC1v2039/PBV1c800, which amplifies part of BC1 ORF, CR, and part of BV1 ORF, gave virus-specific DNA fragments of the sizes expected from a whitefly-transmitted geminivirus. These were cloned and the complete nucleotide (sequences for DNA-A (pToYA, GenBank accession no. U79998) and DNA-B (pToYB, GenBank accession no. U80042) fragments obtained. Nucleotide identity between the CRs (184 nucleotides) was 90%, strongly indicating that those fragments correspond to a bipartite subgroup III geminivirus. PCR with the DNA from infected potato gave the expected size fragment for DNA-A. The partial sequence of the rep gene was 100% identical to the homologous sequence from the PCR fragment from the infected tomato. A search in the GenBank, EMBL, DDBJ, and PDB databases, using the BLAST program, found no identical geminivirus. The highest identity for the CR was 75% to tomato mottle geminivirus-Florida (ToMoV) and 74% to bean golden mosaic virus-Brazil. For the rep gene, the highest identity was 73% to tomato yellow leaf curl virus-Israel, an Old World geminivi-rus, followed by 71% to tomato golden mosaic virus-Brazil (TGMV) and ToMoV. For the cp gene, the highest identity was 86% to TGMV, followed by 83% to squash leaf curl geminivirus. Therefore, we propose the name tomato yellow vein streak geminivirus (ToYVSV) for this distinct virus (2). MenosThe apical growth of about 20% of young tomato plants in observed fields near Campinas, State of São Paulo, Brazil, had yellow streaking of veins. Leaf symptoms developed into patches of yellow mosaic and the leaves became wavy. The whitefly Bemisia tabaci Genn. transmitted a pathogen from the infected tomato plants to healthy tomato and potato plants, reproducing the original symptoms in tomato. The apical leaves of infected potatoes showed yellow or green mottle that developed into leaf distortion with yellow blotches, symptoms indistinguishable from potato-deforming mosaic disease (2). DNA was extracted from these tomato and potato plants (1). Using DNA from the infected tomato plant, polymerase chain reaction (PCR) with the degenerate primer pair PAC1v1978/ PAV1c715 (1), which amplifies part of the rep gene (AC1 ORF), the common region (CR), and part of the cp gene (AV1 ORF), and with the primer pair PBC1v2039/PBV1c800, which amplifies part of BC1 ORF, CR, and part of BV1 ORF, gave virus-specific DNA fragments of the sizes expected from a whitefly-transmitted geminivirus. These were cloned and the complete nucleotide (sequences for DNA-A (pToYA, GenBank accession no. U79998) and DNA-B (pToYB, GenBank accession no. U80042) fragments obtained. Nucleotide identity between the CRs (184 nucleotides) was 90%, strongly indicating that those fragments correspond to a bipartite subgroup III geminivirus. PCR with the DNA from infected potato gave the expected size fragment for DNA... Mostrar Tudo |
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Palavras-Chave: |
Brasil; Diseases; Geminivirus; Ocorrencia; Ocurrence; São Paulo. |
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Thesagro: |
Bemisia tabaci; Doença; Doença de planta; Lycopersicon Esculentum; Tomate; Vírus. |
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Thesaurus Nal: |
Brazil; tomatoes. |
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Categoria do assunto: |
--
H Saúde e Patologia |
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Marc: |
LEADER 03106naa a2200337 a 4500
001 1757312
005 1997-05-12
008 1997 bl uuuu u00u1 u #d
100 1 $aFARIA, J. C.
245 $aA new geminivirus associated with tomato in the State of Sao Paulo, Brazil.
260 $c1997
500 $aNotes.
520 $aThe apical growth of about 20% of young tomato plants in observed fields near Campinas, State of São Paulo, Brazil, had yellow streaking of veins. Leaf symptoms developed into patches of yellow mosaic and the leaves became wavy. The whitefly Bemisia tabaci Genn. transmitted a pathogen from the infected tomato plants to healthy tomato and potato plants, reproducing the original symptoms in tomato. The apical leaves of infected potatoes showed yellow or green mottle that developed into leaf distortion with yellow blotches, symptoms indistinguishable from potato-deforming mosaic disease (2). DNA was extracted from these tomato and potato plants (1). Using DNA from the infected tomato plant, polymerase chain reaction (PCR) with the degenerate primer pair PAC1v1978/ PAV1c715 (1), which amplifies part of the rep gene (AC1 ORF), the common region (CR), and part of the cp gene (AV1 ORF), and with the primer pair PBC1v2039/PBV1c800, which amplifies part of BC1 ORF, CR, and part of BV1 ORF, gave virus-specific DNA fragments of the sizes expected from a whitefly-transmitted geminivirus. These were cloned and the complete nucleotide (sequences for DNA-A (pToYA, GenBank accession no. U79998) and DNA-B (pToYB, GenBank accession no. U80042) fragments obtained. Nucleotide identity between the CRs (184 nucleotides) was 90%, strongly indicating that those fragments correspond to a bipartite subgroup III geminivirus. PCR with the DNA from infected potato gave the expected size fragment for DNA-A. The partial sequence of the rep gene was 100% identical to the homologous sequence from the PCR fragment from the infected tomato. A search in the GenBank, EMBL, DDBJ, and PDB databases, using the BLAST program, found no identical geminivirus. The highest identity for the CR was 75% to tomato mottle geminivirus-Florida (ToMoV) and 74% to bean golden mosaic virus-Brazil. For the rep gene, the highest identity was 73% to tomato yellow leaf curl virus-Israel, an Old World geminivi-rus, followed by 71% to tomato golden mosaic virus-Brazil (TGMV) and ToMoV. For the cp gene, the highest identity was 86% to TGMV, followed by 83% to squash leaf curl geminivirus. Therefore, we propose the name tomato yellow vein streak geminivirus (ToYVSV) for this distinct virus (2).
650 $aBrazil
650 $atomatoes
650 $aBemisia tabaci
650 $aDoença
650 $aDoença de planta
650 $aLycopersicon Esculentum
650 $aTomate
650 $aVírus
653 $aBrasil
653 $aDiseases
653 $aGeminivirus
653 $aOcorrencia
653 $aOcurrence
653 $aSão Paulo
700 1 $aSOUZA-DIAS, J.A.C.
700 1 $aSLACK, S. A.
700 1 $aMAXWELL, D. P.
773 $tPlant Disease$gv.81, n.4, p.423, 1997.
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Registro original: |
Embrapa Hortaliças (CNPH) |
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| 2. |  | ARAÚJO, A. P. F.; GOMES, T. G.; ANDRADE, C. F. S.; MACHADO, E. R.; SIQUEIRA, F. G. de; MENDONCA, S. Perfil glicêmico de camundongos diabéticos alimentados com Agaricus blazei In: SIMPÓSIO INTERNACIONAL SOBRE COGUMELOS NO BRASIL, 8.; SIMPÓSIO NACIONAL SOBRE COGUMELOS COMESTÍVEIS, 7., 2015, Sorocaba, SP. Anais ... Sorocaba: Uniso, 2015. Pôster 34| Tipo: Resumo em Anais de Congresso |
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| 4. | | MACHADO, A. E. V.; ARAÚJO, A. P. F.; URBEN, A. F.; HELM, C. V.; COSTA, A. M.; CAMELINI, C. M.; MENDONCA, S.; SIQUEIRA, F. G. Casca de coco verde como matéria-prima para o cultivo de Pleurotus spp. In: SIMPÓSIO INTERNACIONAL SOBRE COGUMELOS NO BRASIL, 8.; SIMPÓSIO NACIONAL SOBRE COGUMELOS COMESTÍVEIS, 7., 2015, Sorocaba. Anais do simpósio. Sorocaba: UNISO; Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2015. p. 186-187. Pôster 12.| Tipo: Nota Técnica/Nota Científica |
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| 5. | | MACHADO, A. E. V.; ARAÚJO, A. P. F.; URBEN, A. F.; HELM, C. V.; COSTA, A. M.; CAMELINI, C. M.; MENDONCA, S.; SIQUEIRA, F. G. Casca de coco verde como matéria-prima para o cultivo de Pleurotus spp. In: SIMPÓSIO INTERNACIONAL SOBRE COGUMELOS NO BRASIL, 8.; SIMPÓSIO NACIONAL SOBRE COGUMELOS COMESTÍVEIS, 7., 2015, Sorocaba. Anais do simpósio. Sorocaba: UNISO; Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2015. p. 186-187. Pôster 12.| Tipo: Resumo em Anais de Congresso |
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| Registros recuperados : 9 | |
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