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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/01/1998 |
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Data da última atualização: |
12/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TORRES, G. A.; PARENTONI, S. N.; LOPES, M. A.; PAIVA, E. |
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Afiliação: |
EMBRAPA/CNPMS; SIDNEY NETTO PARENTONI, CNPMS. |
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Título: |
A search for RFLP markers to identify genes for aluminum tolerance in maize. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Revista Brasileira de Genética, Ribeirão Preto, v. 20, n. 3, p. 459-465, 1997. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to identify restriction fragment length polymorphism (RFLP) markers linked to QTLs that control aluminum (Al) tolerance in maize. The strategy used was bulked segregation analysis (BSA) and the genetic material utilized was an F2 population derived from a cross between the Al-susceptible inbred line L53 and Al-tolerant inbred line L1327. Both lines were developed at the National Maize and Sorghum Research Center - CNPMS/EMBRAPA. The F2 population of 1554 individuals was evaluated in a nutrient solution containing a toxic concentration of Al and relative seminal root length (RSRL) was used as a phenotype measured tolerance. The RSRL frequency distribution was continuous, but skewed towards Al-susceptible individuals. Seedlings of the F2 population which scored the highest and the lowest RSRL values were transplanted to the field and subsequently selfed to obtain F3 families. Thirty F3 families (15 Al-susceptible and 15 Al-tolerant) were evaluated in nutrient solution, using an incomplete block design, to identify those with the smallest variances for aluminum tolerance and susceptibility. Six Al-susceptible and five Al-tolerant F3 families were chosen to construct one pool of Al-susceptible individuals, and another of Al-tolerant, herein refered as "bulks", based on average values of RSRL and genetic variance. One hundred and thirteen probes were selected, with an average interval of 30 cM, covering the 10 maize chromosomes. These were tested for their ability to discriminate the parental lines. Fifty-four of these probes were polymorphic, with 46 showing codominance. These probes were hybridized with DNA from the two contrasting bulks. Three RFLPs on chromosome 8 distinguished the bulks on the basis of band intensity. DNA of individuals from the bulks was hybridized with these probes and showed the presence of heterozygous individuals in each bulk. These results suggest that in maize there is a region related to aliminum tolerance on chromosome 8. MenosThe objective of this study was to identify restriction fragment length polymorphism (RFLP) markers linked to QTLs that control aluminum (Al) tolerance in maize. The strategy used was bulked segregation analysis (BSA) and the genetic material utilized was an F2 population derived from a cross between the Al-susceptible inbred line L53 and Al-tolerant inbred line L1327. Both lines were developed at the National Maize and Sorghum Research Center - CNPMS/EMBRAPA. The F2 population of 1554 individuals was evaluated in a nutrient solution containing a toxic concentration of Al and relative seminal root length (RSRL) was used as a phenotype measured tolerance. The RSRL frequency distribution was continuous, but skewed towards Al-susceptible individuals. Seedlings of the F2 population which scored the highest and the lowest RSRL values were transplanted to the field and subsequently selfed to obtain F3 families. Thirty F3 families (15 Al-susceptible and 15 Al-tolerant) were evaluated in nutrient solution, using an incomplete block design, to identify those with the smallest variances for aluminum tolerance and susceptibility. Six Al-susceptible and five Al-tolerant F3 families were chosen to construct one pool of Al-susceptible individuals, and another of Al-tolerant, herein refered as "bulks", based on average values of RSRL and genetic variance. One hundred and thirteen probes were selected, with an average interval of 30 cM, covering the 10 maize chromosomes. These were tested f... Mostrar Tudo |
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Palavras-Chave: |
Maize; RFLP; Tolerance; Tolerancia. |
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Thesagro: |
Alumínio; Milho; Zea Mays. |
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Thesaurus Nal: |
aluminum. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/43456/1/Search-RFLP.pdf
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Marc: |
LEADER 02698naa a2200253 a 4500
001 1478779
005 2018-06-12
008 1997 bl uuuu u00u1 u #d
100 1 $aTORRES, G. A.
245 $aA search for RFLP markers to identify genes for aluminum tolerance in maize.$h[electronic resource]
260 $c1997
520 $aThe objective of this study was to identify restriction fragment length polymorphism (RFLP) markers linked to QTLs that control aluminum (Al) tolerance in maize. The strategy used was bulked segregation analysis (BSA) and the genetic material utilized was an F2 population derived from a cross between the Al-susceptible inbred line L53 and Al-tolerant inbred line L1327. Both lines were developed at the National Maize and Sorghum Research Center - CNPMS/EMBRAPA. The F2 population of 1554 individuals was evaluated in a nutrient solution containing a toxic concentration of Al and relative seminal root length (RSRL) was used as a phenotype measured tolerance. The RSRL frequency distribution was continuous, but skewed towards Al-susceptible individuals. Seedlings of the F2 population which scored the highest and the lowest RSRL values were transplanted to the field and subsequently selfed to obtain F3 families. Thirty F3 families (15 Al-susceptible and 15 Al-tolerant) were evaluated in nutrient solution, using an incomplete block design, to identify those with the smallest variances for aluminum tolerance and susceptibility. Six Al-susceptible and five Al-tolerant F3 families were chosen to construct one pool of Al-susceptible individuals, and another of Al-tolerant, herein refered as "bulks", based on average values of RSRL and genetic variance. One hundred and thirteen probes were selected, with an average interval of 30 cM, covering the 10 maize chromosomes. These were tested for their ability to discriminate the parental lines. Fifty-four of these probes were polymorphic, with 46 showing codominance. These probes were hybridized with DNA from the two contrasting bulks. Three RFLPs on chromosome 8 distinguished the bulks on the basis of band intensity. DNA of individuals from the bulks was hybridized with these probes and showed the presence of heterozygous individuals in each bulk. These results suggest that in maize there is a region related to aliminum tolerance on chromosome 8.
650 $aaluminum
650 $aAlumínio
650 $aMilho
650 $aZea Mays
653 $aMaize
653 $aRFLP
653 $aTolerance
653 $aTolerancia
700 1 $aPARENTONI, S. N.
700 1 $aLOPES, M. A.
700 1 $aPAIVA, E.
773 $tRevista Brasileira de Genética, Ribeirão Preto$gv. 20, n. 3, p. 459-465, 1997.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 9.568 | |
| 201. |  | COELHO, G. T. C. P.; REZENDE, R. K. S.; TORGA, P. P.; PETRILLO, C. P.; CARNEIRO, A. A.; CARNEIRO, N. P.; PINHO, R. G. V.; PAIVA, R. Embriogênese somática de linhagens tropicais de milho (Zea mays) em diferentes concentrações de 2,4-D. Horticultura Brasileira, v. 23, n. 2, p. 600, ago. 2005. Suplemento. Edição dos resumos do 45º Congresso Brasileiro de Olericultura; 15º Congresso Brasileiro de Floricultura e Plantas Ornamentais; 2º Congresso Brasileiro de Cultura de Tecidos de Plantas realizado em Fortaleza.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 203. | | SENA, M. R.; GUIMARAES, P. E. de O.; SOUZA, J. C.; COSTA, L. P.; SCHAFFERT, R. E.; RIBEIRO, P. E. de A.; PACHECO, C. A. P. Estabilidade dos teores de minerais em grãos de variedades de milho. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 4., 2007, São Lourenço. Melhoramento de plantas e agronegócio: Anais... Lavras: UFLA: SBMP, 2007. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 204. | | GOMES, E. A.; OLIVEIRA, C.; CARNEIRO, N. P.; MARRIEL, I. E.; SA, N.; SCOTTO, M.; SCHAFFERT, R. E.; GUIMARAES, C.; ALVES, V. M. C. The effect of Zea mays genotypes contrasting for phosphorus efficiency on mycorrhizal diversity analyzed by denaturing gradient gel electrophoresis (DGGE) and DNA cloning/sequencing. In: INTERNATIONAL CONFERENCE RHIZOSPHERE , 2., 2007, Montpellier. Book of abstracts... Montpellier: INRA, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 206. | | HOEKENGA, O. A.; BUCKLER, E.; MARON, L.; MAGALHAES, J. V. de; KIRST, M.; KRILL, A.; LYI, S. M.; ROSE, J.; THANNHAUSER, T.; KOCHIAN, L. Joint linkage-association analysis of aluminum tolerance in maize. In: INTERNATIONAL PLANT & ANIMAL GENOMES CONFERENCE, 15., 2007, San Diego, CA. [Proceedings...]. [S. l.: s.n.], 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 207. | | LIGEYO, D. O.; GUDU, S.; OBURA, P.; OKALEBO, J. R.; OTHIENO, C. O.; OMBAKHO, G. A.; SCHAFFERT, R. E.; ALVES, V. M. C.; MAGALHAES, J. V. de; GAMA, E. E. G. e. Improvement of Kenyan maize germplasm for adaptability to low soil pH. In: GRANTEE CONFERENCE FROM PARTNERSHIPS TO COMMUNITY: THE ARC OF CHANGE IN THE CCRP, 2004, Vaals, The Netherlands. [Proceedings]. [Minneapolis]: The McKnight Foundation, 2004.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 209. | | CHIESA, A. C. M.; SISMEIRO, M. N. S.; BROCCO, L. A. F.; EDUARDO, J. A.; HOSHINO, A. T.; SARTORI, L. C.; PASINI, A.; ROGGIA, S. Injúria de Spodoptera frugiperda em milho Bt em sucessão a soja transgênica e submetido a diferentes manejos fitossanitários. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 25., 2014, Goiânia. Entomologia integrada à sociedade para o desenvolvimento sustentável: anais. [Londrina]: SEB, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 211. | | ORSO, L.; MACHADO, J. R. de A.; GEHLEN, A.; GAUZE, J. Interação genótipo x local em híbridos de milho. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 13.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 10., 2018, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2018. p. 32. Graduação.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 218. | | RIOS, S. A.; CARDOSO, W. S.; PAES, M. C. D.; BORÉM, A.; GUIMARAES, P. E. de O.; SCHAFFERT, R. E.; GOMES, A. F. Influência da cor no perfil de carotenóides de grãos de milho para geração de cultivares biofortificados. In: REUNIÃO ANUAL DE BIOFORTIFICAÇÃO NO BRASIL, 2., 2007, Niterói. posteres. Rio de Janeiro: Embrapa Agroindustria de Alimentos, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 219. | | RIOS, S. A.; CARDOSO, W. S.; PAES, M. C. D.; BOREM, A.; GUIMARAES, P. E. de O.; SCHAFFERT, R. E.; TEIXEIRA, F. F.; GOMES, A. F. Influência da cor no perfil de carotenóides de grãos de milho para geração de cultivares biofortificados. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 4., 2007, São Lourenço. Melhoramento de plantas e agronegócio: Anais...Lavras: UFLA: SBMP, 2007. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 220. | | OLIVEIRA JUNIOR, G. I. de; MORAES, V. B. de; NAKAJIMA, V. M.; SILVA, C. O. da; MORAES, E. A.; PAES, M. C. D.; COSTA, N. M. B. Influência da fibra de pericarpo de milho na biodisponibilidade de ferro em ratos. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DOS ALIMENTOS, 21.; SEMINÁRIO LATINOAMERICANO E DO CARIBE DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 15., 2008, Belo Horizonte. Anais... Belo Horizonte: Sociedade Brasileira de Ciência e Tecnologia de Alimentos, 2008. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 9.568 | |
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