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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Ocidental. Para informações adicionais entre em contato com cpaa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
10/09/2012 |
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Data da última atualização: |
12/09/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CHEN, S. X.; ALMEIDA, F. F. L.; ANDERSSON, E.; TARANGER, G. L.; SCHMIDT, R.; SCHULZ, R. W.; BOGERD, J. |
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Afiliação: |
Shi X. Chen, State Key Laboratory of Marine Environmental Science, Xiamen University; FERNANDA LOUREIRO ALMEIDA OSULLIVAN, CPAA; Eva Andersson, Research Group Reproduction and Growth, Institute of Marine Research; Geir Lasse Taranger, Research Group Reproduction and Growth, Institute of Marine Research; Ruben Schmidt, Department of Biology, Faculty of Science, Utrecht University; Rüdiger W. Schulz, Department of Biology, Faculty of Science, Utrecht University; Jan Bogerd, Department of Biology, Faculty of Science, Utrecht University. |
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Título: |
Cloning, pharmacological characterization and expression analysis of Atlantic cod (Gadus morhua, L.) nuclear progesterone receptor. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
General and Comparative Endocrinology, v. 179, p. 71-77, 2012. |
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DOI: |
http://dx.doi.org/10.1016/j.ygcen.2012.07.022 |
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Idioma: |
Inglês |
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Conteúdo: |
To better understand the role(s) of progesterone in fish spermatogenesis, we cloned the nuclear progesterone receptor (Pgr) of Atlantic cod. The open-reading frame of the cod pgr consists of 2076 bp, coding for a 691-amino acids-long protein that shows the highest similarity with other piscine Pgr proteins. Functional characterization of the receptor expressed in mammalian cells revealed that the cod Pgr exhibited progesterone-specific, dose-dependent induction of reporter gene expression, with 17a,20bdihydroxy- 4-pregnen-3-one (DHP), a typical piscine progesterone, showing the highest potency in activating the receptor. During ontogenesis, the pgr mRNA was undetectable in embryo?s 24 h after fertilization, but became detectable 4 days after fertilization. During the larval stage, the expression levels increased steadily with the development of the larvae. In adult fish, pgr was predominantly expressed in gonads of both sexes. During the onset of puberty, testicular pgr transcript levels started to increase during rapid spermatogonial proliferation, and peaked when spermiation started. In situ hybridization studies using testis tissue during the rapid growth phase containing all germ cell stages indicated that in cod, pgr mRNA is predominantly located in Sertoli cells that are in contact with proliferating spermatogonia. Taken together, our data suggests that the Pgr is involved in mediating progestagen stimulation of the mitotic expansion of spermatogonia, and in processes associated with the spermiation/spawning period in Atlantic cod. MenosTo better understand the role(s) of progesterone in fish spermatogenesis, we cloned the nuclear progesterone receptor (Pgr) of Atlantic cod. The open-reading frame of the cod pgr consists of 2076 bp, coding for a 691-amino acids-long protein that shows the highest similarity with other piscine Pgr proteins. Functional characterization of the receptor expressed in mammalian cells revealed that the cod Pgr exhibited progesterone-specific, dose-dependent induction of reporter gene expression, with 17a,20bdihydroxy- 4-pregnen-3-one (DHP), a typical piscine progesterone, showing the highest potency in activating the receptor. During ontogenesis, the pgr mRNA was undetectable in embryo?s 24 h after fertilization, but became detectable 4 days after fertilization. During the larval stage, the expression levels increased steadily with the development of the larvae. In adult fish, pgr was predominantly expressed in gonads of both sexes. During the onset of puberty, testicular pgr transcript levels started to increase during rapid spermatogonial proliferation, and peaked when spermiation started. In situ hybridization studies using testis tissue during the rapid growth phase containing all germ cell stages indicated that in cod, pgr mRNA is predominantly located in Sertoli cells that are in contact with proliferating spermatogonia. Taken together, our data suggests that the Pgr is involved in mediating progestagen stimulation of the mitotic expansion of spermatogonia, and in processes ... Mostrar Tudo |
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Palavras-Chave: |
Atlantic cod; Nuclear progesterone receptor. |
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Thesaurus Nal: |
spermatogenesis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02333naa a2200241 a 4500
001 1933292
005 2012-09-12
008 2012 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.ygcen.2012.07.022$2DOI
100 1 $aCHEN, S. X.
245 $aCloning, pharmacological characterization and expression analysis of Atlantic cod (Gadus morhua, L.) nuclear progesterone receptor.
260 $c2012
520 $aTo better understand the role(s) of progesterone in fish spermatogenesis, we cloned the nuclear progesterone receptor (Pgr) of Atlantic cod. The open-reading frame of the cod pgr consists of 2076 bp, coding for a 691-amino acids-long protein that shows the highest similarity with other piscine Pgr proteins. Functional characterization of the receptor expressed in mammalian cells revealed that the cod Pgr exhibited progesterone-specific, dose-dependent induction of reporter gene expression, with 17a,20bdihydroxy- 4-pregnen-3-one (DHP), a typical piscine progesterone, showing the highest potency in activating the receptor. During ontogenesis, the pgr mRNA was undetectable in embryo?s 24 h after fertilization, but became detectable 4 days after fertilization. During the larval stage, the expression levels increased steadily with the development of the larvae. In adult fish, pgr was predominantly expressed in gonads of both sexes. During the onset of puberty, testicular pgr transcript levels started to increase during rapid spermatogonial proliferation, and peaked when spermiation started. In situ hybridization studies using testis tissue during the rapid growth phase containing all germ cell stages indicated that in cod, pgr mRNA is predominantly located in Sertoli cells that are in contact with proliferating spermatogonia. Taken together, our data suggests that the Pgr is involved in mediating progestagen stimulation of the mitotic expansion of spermatogonia, and in processes associated with the spermiation/spawning period in Atlantic cod.
650 $aspermatogenesis
653 $aAtlantic cod
653 $aNuclear progesterone receptor
700 1 $aALMEIDA, F. F. L.
700 1 $aANDERSSON, E.
700 1 $aTARANGER, G. L.
700 1 $aSCHMIDT, R.
700 1 $aSCHULZ, R. W.
700 1 $aBOGERD, J.
773 $tGeneral and Comparative Endocrinology$gv. 179, p. 71-77, 2012.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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| Registros recuperados : 199 | |
| 2. |  | RODRIGUES, T. E. Solos da Amazônia. In: ALVAREZ V., V. H.; FONTES, L. E. F.; FONTES, M. P. F. O solo nos grandes domínios morfoclimáticos do Brasil e o desenvolvimento sustentado. Viçosa: SBCS: UFV, 1996. p. 19-60. il.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 5. | | RIBEIRO, S. B.; RODRIGUES, T. E. Caracterização morfológica, física e química dos solos e a dinâmica dessas características em diferentes sistemas de manejo e uso da terra em áreas de pequenos produtores. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA UFRA, 3.; SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 9., 2006, Belém, PA. Agricultura sustentável na Amazônia: anais. Belém, PA: UFRA: Embrapa Amazônia Oriental, 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 12. | | BAENA, A. R. C.; RODRIGUES, T. E. Solos. In: DUARTE, M. de L. R. Cultivo da pimenteira-do-reino na Região Norte. Belém, PA: Embrapa Amazônia Oriental, 2004. p. 23-28. (Embrapa Amazônia Oriental. Sistemas de produção, 1).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 199 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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