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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
06/08/2025 |
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Data da última atualização: |
06/08/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, I. R.; BATISTA, M. da M.; LIMA, J. D.; RIBEIRO, D. G.; MENDES, P. da N.; LUCENA, V. dos S.; FREIRE, E. V. S. A.; FONTES, W.; FERNANDEZ, D.; REIS, A. M. dos. |
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Afiliação: |
IVONALDO REIS SANTOS, UNIVERSIDADE DE BRASÍLIA; MILENA DA MOTA BATISTA; JONATHAN DIAS LIMA, UNIVERSIDADE DE BRASÍLIA; DAIANE GONZAGA RIBEIRO, UNIVERSIDADE DE BRASÍLIA; POLLYANA DA NÓBREGA MENDES; VÍVIAN DOS SANTOS LUCENA, UNIVERSIDADE DE BRASÍLIA; ERIKA VALERIA SALIBA ALBUQUERQUE FREIRE, CENARGEN; WAGNER FONTES, UNIVERSIDADE DE BRASÍLIA; DIANA FERNANDEZ, UNIV. MONTPELLIER; ANGELA MEHTA DOS REIS, CENARGEN. |
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Título: |
Coffea arabica susceptibility genes identified by proteomic analysis during coffee-Hemileia vastatrix interaction. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Physiological and Molecular Plant Pathology, v. 139, 102832, 2025. |
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DOI: |
https://doi.org/10.1016/j.pmpp.2025.102832 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Érika Valeria Saliba Albuquerque; Angela Mehta. |
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Conteúdo: |
Coffea arabica L. is one of the most important cash crops worldwide; however, it is highly affected by coffee leaf rust caused by Hemileia vastatrix. A promising alternative to obtain resistance is the use of susceptibility genes of the host plant as targets for gene silencing or knockout. The present study aimed to identify proteins in C. arabica var. Catuaí Amarelo potentially involved in the susceptibility to H. vastatrix using a proteomic approach. Leaves from infected and non-infected (control) coffee plants were collected ten days after inoculation with H. vastatrix. Total proteins were extracted, and the peptides were evaluated by LC-MS/MS. Shotgun proteomics revealed 2.335 proteins, of which 288 were differentially accumulated when infected and non-infected coffee leaf samples were compared. Differential proteins were classified into biological processes associated to pathogen response such as defense, photosynthesis, response to fungus, response to stimuli, carbohydrate metabolism and stress response. To validate the expression of potential genes associated with susceptibility, naturally infected leaves under field conditions were collected. Total RNA was extracted from coffee leaves and submitted to RT-qPCR analysis. The results showed that 6 genes (GSTs, GPx, SOD, Bgluc, ALDOA, and 6GPHD) were upregulated in infected samples, showing a positive correlation with protein accumulation. Overall, this work contributes to a better understanding of the metabolic pathways that are affected during the C. arabica - H. vastatrix susceptible interaction. Furthermore, the knockout of the candidate genes by CRISPR technology can be performed to obtain coffee plants with improved resistance to H. vastatrix. MenosCoffea arabica L. is one of the most important cash crops worldwide; however, it is highly affected by coffee leaf rust caused by Hemileia vastatrix. A promising alternative to obtain resistance is the use of susceptibility genes of the host plant as targets for gene silencing or knockout. The present study aimed to identify proteins in C. arabica var. Catuaí Amarelo potentially involved in the susceptibility to H. vastatrix using a proteomic approach. Leaves from infected and non-infected (control) coffee plants were collected ten days after inoculation with H. vastatrix. Total proteins were extracted, and the peptides were evaluated by LC-MS/MS. Shotgun proteomics revealed 2.335 proteins, of which 288 were differentially accumulated when infected and non-infected coffee leaf samples were compared. Differential proteins were classified into biological processes associated to pathogen response such as defense, photosynthesis, response to fungus, response to stimuli, carbohydrate metabolism and stress response. To validate the expression of potential genes associated with susceptibility, naturally infected leaves under field conditions were collected. Total RNA was extracted from coffee leaves and submitted to RT-qPCR analysis. The results showed that 6 genes (GSTs, GPx, SOD, Bgluc, ALDOA, and 6GPHD) were upregulated in infected samples, showing a positive correlation with protein accumulation. Overall, this work contributes to a better understanding of the metabolic pathways... Mostrar Tudo |
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Palavras-Chave: |
Coffee rust; S-genes. |
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Thesaurus Nal: |
Gene expression; Proteomics. |
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Categoria do assunto: |
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Marc: |
LEADER 02702naa a2200301 a 4500
001 2177753
005 2025-08-06
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.pmpp.2025.102832$2DOI
100 1 $aSANTOS, I. R.
245 $aCoffea arabica susceptibility genes identified by proteomic analysis during coffee-Hemileia vastatrix interaction.$h[electronic resource]
260 $c2025
500 $aNa publicação: Érika Valeria Saliba Albuquerque; Angela Mehta.
520 $aCoffea arabica L. is one of the most important cash crops worldwide; however, it is highly affected by coffee leaf rust caused by Hemileia vastatrix. A promising alternative to obtain resistance is the use of susceptibility genes of the host plant as targets for gene silencing or knockout. The present study aimed to identify proteins in C. arabica var. Catuaí Amarelo potentially involved in the susceptibility to H. vastatrix using a proteomic approach. Leaves from infected and non-infected (control) coffee plants were collected ten days after inoculation with H. vastatrix. Total proteins were extracted, and the peptides were evaluated by LC-MS/MS. Shotgun proteomics revealed 2.335 proteins, of which 288 were differentially accumulated when infected and non-infected coffee leaf samples were compared. Differential proteins were classified into biological processes associated to pathogen response such as defense, photosynthesis, response to fungus, response to stimuli, carbohydrate metabolism and stress response. To validate the expression of potential genes associated with susceptibility, naturally infected leaves under field conditions were collected. Total RNA was extracted from coffee leaves and submitted to RT-qPCR analysis. The results showed that 6 genes (GSTs, GPx, SOD, Bgluc, ALDOA, and 6GPHD) were upregulated in infected samples, showing a positive correlation with protein accumulation. Overall, this work contributes to a better understanding of the metabolic pathways that are affected during the C. arabica - H. vastatrix susceptible interaction. Furthermore, the knockout of the candidate genes by CRISPR technology can be performed to obtain coffee plants with improved resistance to H. vastatrix.
650 $aGene expression
650 $aProteomics
653 $aCoffee rust
653 $aS-genes
700 1 $aBATISTA, M. da M.
700 1 $aLIMA, J. D.
700 1 $aRIBEIRO, D. G.
700 1 $aMENDES, P. da N.
700 1 $aLUCENA, V. dos S.
700 1 $aFREIRE, E. V. S. A.
700 1 $aFONTES, W.
700 1 $aFERNANDEZ, D.
700 1 $aREIS, A. M. dos
773 $tPhysiological and Molecular Plant Pathology$gv. 139, 102832, 2025.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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