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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
27/01/2025 |
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Data da última atualização: |
27/01/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RIBEIRO, D. G.; CARVALHO, J. de O.; SARTORI, R.; MONTEIRO, P. L. J.; FONTES, W.; CASTRO, M. de S.; SOUSA, M. V. de; DODE, M. A. N.; REIS, A. M. dos. |
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Afiliação: |
DAIANE GONZAGA RIBEIRO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; JOSÉ DE OLIVEIRA CARVALHO, FEDERAL UNIVERSITY OF ESPIRITO SANTO; ROBERTO SARTORI, UNIVERSITY OF SÃO PAULO; PEDRO LEOPOLDO JERÔNIMO MONTEIRO, UNIVERSITY OF SÃO PAULO; WAGNER FONTES, UNIVERSITY OF BRASÍLIA; MARIANA DE SOUZA CASTRO, UNIVERSITY OF BRASÍLIA; MARCELO VALLE DE SOUSA, UNIVERSITY OF BRASÍLIA; MARGOT ALVES NUNES DODE, CENARGEN; ANGELA MEHTA DOS REIS, CENARGEN. |
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Título: |
The presence of sexed sperm in bovine oviduct epithelial cells alters the protein profile related to stress and immune response. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Research in Veterinary Science, v. 184, 105522, 2025. |
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DOI: |
https://doi.org/10.1016/j.rvsc.2024.105522 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Angela Mehta. |
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Conteúdo: |
Although sperm sexing technology has progressed considerably in the last decade, there are still challenges to fully understand the reason for the low fertility of sexed sperm. Thus, we aimed to evaluate the effect of sexed and non-sexed sperm on the proteome of bovine oviduct epithelial cells (BOECs). Semen from six Nellore bulls was used and one ejaculate from each bull was collected and separated into three fractions: non-sexed, sexed for X-sperm and sexed for Y-sperm. Previously synchronized females were artificially inseminated with either a pool of non-sexed sperm from 6 sires (NS), or a pool of sexed X and Y sperm from 6 sires (XY) or saline solution (Control). After insemination, animals were slaughtered and oviducts were collected to obtain BOECs samples, which were used for proteomic analysis. The results revealed that the oviductal response on isthmus region to the presence of sperm is different when sexed and non-sexed sperm are used. Sexed sperm seemed to induced a more intense and imbalanced response to several processes, such as oxidative and heat stress, immune response and movement of the oviduct muscle. |
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Palavras-Chave: |
Sperm sexing technology. |
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Thesaurus Nal: |
Animal reproduction; Proteome. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02032naa a2200277 a 4500
001 2172003
005 2025-01-27
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.rvsc.2024.105522$2DOI
100 1 $aRIBEIRO, D. G.
245 $aThe presence of sexed sperm in bovine oviduct epithelial cells alters the protein profile related to stress and immune response.$h[electronic resource]
260 $c2025
500 $aNa publicação: Angela Mehta.
520 $aAlthough sperm sexing technology has progressed considerably in the last decade, there are still challenges to fully understand the reason for the low fertility of sexed sperm. Thus, we aimed to evaluate the effect of sexed and non-sexed sperm on the proteome of bovine oviduct epithelial cells (BOECs). Semen from six Nellore bulls was used and one ejaculate from each bull was collected and separated into three fractions: non-sexed, sexed for X-sperm and sexed for Y-sperm. Previously synchronized females were artificially inseminated with either a pool of non-sexed sperm from 6 sires (NS), or a pool of sexed X and Y sperm from 6 sires (XY) or saline solution (Control). After insemination, animals were slaughtered and oviducts were collected to obtain BOECs samples, which were used for proteomic analysis. The results revealed that the oviductal response on isthmus region to the presence of sperm is different when sexed and non-sexed sperm are used. Sexed sperm seemed to induced a more intense and imbalanced response to several processes, such as oxidative and heat stress, immune response and movement of the oviduct muscle.
650 $aAnimal reproduction
650 $aProteome
653 $aSperm sexing technology
700 1 $aCARVALHO, J. de O.
700 1 $aSARTORI, R.
700 1 $aMONTEIRO, P. L. J.
700 1 $aFONTES, W.
700 1 $aCASTRO, M. de S.
700 1 $aSOUSA, M. V. de
700 1 $aDODE, M. A. N.
700 1 $aREIS, A. M. dos
773 $tResearch in Veterinary Science$gv. 184, 105522, 2025.
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