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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
20/07/2018 |
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Data da última atualização: |
07/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, R. V. dos; VILLALTA-ROMERO, F.; STANISIC, D.; BORRO, L.; NESHICH, G.; TASIC, L. |
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Afiliação: |
RONEY VANDER DOS SANTOS, IQ/Unicamp; FABIAN VILLALTA-ROMERO, IQ/Unicamp, Instituto Tecnológico de Costa Rica; DANIJELA STANISIC, IQ/Unicamp; LUIZ BORRO, Unicamp, CNPTIA; GORAN NESIC, CNPTIA; LJUBICA TASIC, IQ/Unicamp. |
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Título: |
Citrus bioflavonoid, hesperetin, as inhibitor of two thrombin-like snake venom serine proteases isolated from Crotalus simus. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Toxicon, v. 143, p. 36-43, Mar. 2018. |
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DOI: |
https://doi.org/10.1016/j.toxicon.2018.01.005 |
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Idioma: |
Inglês |
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Conteúdo: |
Around 5.5 million people suffer from snakebites per year, with about 400,000 cases with some type of sequelae, such as amputation, and 20,000 to 125,000 cases with the fatal end. Usually, the victim outcome depends on correct, agile and many times in situ intervention based on the proper identification of the snake venom type and its potential effects, among other factors. Therefore, knowledge on the snake venom composition and a research on inhibitors of snake venom target components might ameliorate envenoming dangerous outcome. Herein, two thrombin-like serine proteases from the Crotalus simus snake venom - SVSP1 and SVSP2 - were isolated in two chromatographic steps, using gel filtration and then RP-HPLC. They showed molecular masses of around 31.3 and 24.6 kDa, respectively, and mostly b-sheet secondary structure features. The SVSP1 and SVSP2 were sequenced using tandem mass spectrometry (Q-TOF). Using the known serine protease structure (PDB entry: 4e7n), which was evaluated as homologous to the two target proteins, in silico docking results showed that hesperetin is its excellent inhibitor. Using in vitro tests with the commercial hesperetin, kinetic parameters were obtained for SVSPs against the synthetic substrate BApNA. Obtained results pointed that hesperetin might act as an uncompetitive (SVSP1) or mixed (SVSP2) inhibitor. Also, the fluorescence quenching upon inhibition was observed, as well as, red shift in maximums of around 20 nm, which indicate that the tryptophan residues in the target enzymes suffered conformational changes caused by hesperetin binding. Thus, a naturally occurring flavone that can easily be extracted from oranges might serve as low-cost inhibitor of the investigated snake venom proteases. MenosAround 5.5 million people suffer from snakebites per year, with about 400,000 cases with some type of sequelae, such as amputation, and 20,000 to 125,000 cases with the fatal end. Usually, the victim outcome depends on correct, agile and many times in situ intervention based on the proper identification of the snake venom type and its potential effects, among other factors. Therefore, knowledge on the snake venom composition and a research on inhibitors of snake venom target components might ameliorate envenoming dangerous outcome. Herein, two thrombin-like serine proteases from the Crotalus simus snake venom - SVSP1 and SVSP2 - were isolated in two chromatographic steps, using gel filtration and then RP-HPLC. They showed molecular masses of around 31.3 and 24.6 kDa, respectively, and mostly b-sheet secondary structure features. The SVSP1 and SVSP2 were sequenced using tandem mass spectrometry (Q-TOF). Using the known serine protease structure (PDB entry: 4e7n), which was evaluated as homologous to the two target proteins, in silico docking results showed that hesperetin is its excellent inhibitor. Using in vitro tests with the commercial hesperetin, kinetic parameters were obtained for SVSPs against the synthetic substrate BApNA. Obtained results pointed that hesperetin might act as an uncompetitive (SVSP1) or mixed (SVSP2) inhibitor. Also, the fluorescence quenching upon inhibition was observed, as well as, red shift in maximums of around 20 nm, which indicate that the try... Mostrar Tudo |
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Palavras-Chave: |
Análise in silico; Análise in vitro; Crotalus simus; Inibição enzimática; Snake venom serine proteases; Veneno de cobra. |
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Thesaurus Nal: |
Enzyme inhibition; Hesperetin. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02656naa a2200289 a 4500
001 2093443
005 2020-01-07
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.toxicon.2018.01.005$2DOI
100 1 $aSANTOS, R. V. dos
245 $aCitrus bioflavonoid, hesperetin, as inhibitor of two thrombin-like snake venom serine proteases isolated from Crotalus simus.$h[electronic resource]
260 $c2018
520 $aAround 5.5 million people suffer from snakebites per year, with about 400,000 cases with some type of sequelae, such as amputation, and 20,000 to 125,000 cases with the fatal end. Usually, the victim outcome depends on correct, agile and many times in situ intervention based on the proper identification of the snake venom type and its potential effects, among other factors. Therefore, knowledge on the snake venom composition and a research on inhibitors of snake venom target components might ameliorate envenoming dangerous outcome. Herein, two thrombin-like serine proteases from the Crotalus simus snake venom - SVSP1 and SVSP2 - were isolated in two chromatographic steps, using gel filtration and then RP-HPLC. They showed molecular masses of around 31.3 and 24.6 kDa, respectively, and mostly b-sheet secondary structure features. The SVSP1 and SVSP2 were sequenced using tandem mass spectrometry (Q-TOF). Using the known serine protease structure (PDB entry: 4e7n), which was evaluated as homologous to the two target proteins, in silico docking results showed that hesperetin is its excellent inhibitor. Using in vitro tests with the commercial hesperetin, kinetic parameters were obtained for SVSPs against the synthetic substrate BApNA. Obtained results pointed that hesperetin might act as an uncompetitive (SVSP1) or mixed (SVSP2) inhibitor. Also, the fluorescence quenching upon inhibition was observed, as well as, red shift in maximums of around 20 nm, which indicate that the tryptophan residues in the target enzymes suffered conformational changes caused by hesperetin binding. Thus, a naturally occurring flavone that can easily be extracted from oranges might serve as low-cost inhibitor of the investigated snake venom proteases.
650 $aEnzyme inhibition
650 $aHesperetin
653 $aAnálise in silico
653 $aAnálise in vitro
653 $aCrotalus simus
653 $aInibição enzimática
653 $aSnake venom serine proteases
653 $aVeneno de cobra
700 1 $aVILLALTA-ROMERO, F.
700 1 $aSTANISIC, D.
700 1 $aBORRO, L.
700 1 $aNESHICH, G.
700 1 $aTASIC, L.
773 $tToxicon$gv. 143, p. 36-43, Mar. 2018.
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Embrapa Agricultura Digital (CNPTIA) |
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| Registros recuperados : 59 | |
| 1. |  | COLETTI, A. J.; MONTEIRO, R. A. C.; LAZARINI, E. Consórcio milho x braquiária em sistemas integrados de produção de leite. In: FARIAS NETO, A. L. de; NASCIMENTO, A. F. do; ROSSONI, A. L.; MAGALHÃES, C. A. de S.; ITUASSU, D. R.; HOOGERHEIDE, E. S. S.; IKEDA, F. S.; FERNANDES JUNIOR, F.; FARIA, G. R.; ISERNHAGEN, I.; VENDRUSCULO, L. G.; MORALES, M. M.; CARNEVALLI, R. A. (Ed.). Embrapa Agrossilvipastoril: primeiras contribuições para o desenvolvimento de uma agropecuária sustentável. Brasília, DF: Embrapa, 2019. pt. 4, cap. 29, p. 341-345.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 3. | | MONTEIRO, R. A. C.; BEHLING, M.; TONINI, H.; LULU, J.; COLETTI, A. J.; SHIRATSUCHI, L. S. Integração entre a Lavoura, a Pecuária e a Floresta: ciência e arte. In: SIMPÓSIO BRASILEIRODE AGROPECUÁRIA SUSTENTÁVEL., 8.; INTERNATIONAL CONFERENCE ON SUSTAINABLE AGRICULTURE, 5., 2016. Sinop. Ciência, tecnologia e inovação para o desenvolvimento sustentável das novas fronteiras agrícolas: anais. Sinop: [SIMBRAS], 2016. 123-140. SIMBRAS. 18 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 4. | | GEREMIA, E. V.; CRESTANI, S.; SILVA, S. C. da; MONTEIRO, R. A. C. Comportamento ingestivo e valor nutritivo de pastagens no sistema de integração lavoura-pecuária-floresta. In: FARIAS NETO, A. L. de; NASCIMENTO, A. F. do; ROSSONI, A. L.; MAGALHÃES, C. A. de S.; ITUASSU, D. R.; HOOGERHEIDE, E. S. S.; IKEDA, F. S.; FERNANDES JUNIOR, F.; FARIA, G. R.; ISERNHAGEN, I.; VENDRUSCULO, L. G.; MORALES, M. M.; CARNEVALLI, R. A. (Ed.). Embrapa Agrossilvipastoril: primeiras contribuições para o desenvolvimento de uma agropecuária sustentável. Brasília, DF: Embrapa, 2019. pt. 4, cap. 32, p. 356-359.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 5. | | SCHNEIDER, K. E.; COLETTI, A. J.; ENDLER, T. B.; VERDADEIRO, W. O.; MONTEIRO, R. A. C. Desenvolvimento vegetativo do milho consorciado com densidades de semeadura do capim-ruziziensis em sistema integrado de produção. In: ENCONTRO DE CIÊNCIA E TECNOLOGIAS AGROSSUSTENTÁVEIS, 2.; JORNADA CIENTÍFICA DA EMBRAPA AGROSSILVIPASTORIL, 7., 2018. Sinop, MT. Resumos... Sinop, MT: Embrapa Agrossilpastoril, 2018. p. 73-77.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 15. | | GIUSTINA, C. D.; MONTEIRO, R. A. C.; CARVALHO, C. A. B. de; PEREIRA, F. das C.; MENDES, C. A. Interceptação luminosa e área de copa de frutíferas em sistemas silvipastoris. In: ENCONTRO DE CIÊNCIA E TECNOLOGIAS AGROSSUSTENTÁVEIS, 2.; JORNADA CIENTÍFICA DA EMBRAPA AGROSSILVIPASTORIL, 7., 2018. Sinop, MT. Resumos... Sinop, MT: Embrapa Agrossilpastoril, 2018. p. 101-104.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 16. | | CRESTANI, S.; GEREMIA, E. V.; CABALLERO, J. D.; MONTEIRO, R. A. C.; MONTAGNER, D. B.; SILVA, S. C. da. Effects of the deferment period on chemical composition of Piatã grass in silvipastoral systems. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 53., 2016, Gramado. Produção animal para as novas gerações: anais. Gramado: SBZ, 2016. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 19. | | PEREIRA, F.; MONTEIRO, R. A. C.; GIUSTINA, C. D.; XAVIER, D. B.; BOMFIM, L. K. V. de. Desempenho de bezerros leiteiros em sistemas silvipastoris com frutíferas. In: ENCONTRO DE CIÊNCIA E TECNOLOGIAS AGROSSUSTENTÁVEIS, 2.; JORNADA CIENTÍFICA DA EMBRAPA AGROSSILVIPASTORIL, 7., 2018. Sinop, MT. Resumos... Sinop, MT: Embrapa Agrossilpastoril, 2018. p. 96-100.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| Registros recuperados : 59 | |
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