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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
15/02/2008 |
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Data da última atualização: |
24/07/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
AGUIAR-PERECIN, M. L. R.; MONDIN, M.; SANTOS-SEREJO, J. A. dos; VIDA, A. C. F.; MOLINA, S. C. M. |
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Afiliação: |
Margarida L. R. Aguiar-Perecin, USP; Mateus Mondin, USP; Janay Almeida dos Santos-Serejo, CNPMF; Ana C. F. Vida, USP; Silvia C. M. Molina, USP. |
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Título: |
Analysis of karyotype variability of tropical maize inbred lines and hybrids using FISH of tandemly repeated DNA sequences to identify somatic chromosomes. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In: Chomosome Research, Netherlands, v. 15, n. 2, p. 41 jul. 2007. Supplement. P.088 |
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ISSN: |
0967-3849 |
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Idioma: |
Inglês |
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Notas: |
Edição do 16 INTERNATIONAL CHROMOSOME CONFERENCE, Abstracts...Amsterdam, ago. 2007. Editado por Hans de Jong, Hans Tanke and Paul Fransz. DOI: 10.1007/s10577 ISSN 0967-3849 em papel |
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Conteúdo: |
The analysis of pachytene chromosomes has greatly contributed to maize cytogenetics. Recently, FISH procedures using repeated DNA sequences have been described and improved the identification of somatic chromosomes. Due to the polymorphism of knobs and repetitive DNA sequences, karyotype diversity is observed among varieties. Mitotic chromosome analysis is valuable for the screening of several individuals, and studies of evolution. We investigaded root-tip chromosomes of inbred lines and hybrids, derived from a tropical variety (Cateto races), using the following sequences as probes: subtelomeric 4-12-1 clone (PNAS 101, 13555), knob 180-bp sequence, 5S rDNA, centromeric satellite 4 (Cent 4). Pachytene chromosomes and C-banded mitotic metaphases were previously analysed, and except for chromosomes 2, 4 and 5, most chromosomes were distinguished by their length, arm ratio or presence of knobs. We identified chromosomes 2 and 4 using probes of 5S rDNA (2L) and Cent4, respectively, but their arm ratios were atypical. Signals of the 180-bp sequence were correspondent to knobs detected by C-banding, and additional small signals were found at 1S, 6S and 9S, located at arm tips, a feature also found in other varieties. The subtelomeric sequence was observed at 1S, 2S, 4SL, 5S, 8L. One line was crossed with KYS, an inbred extensively investigated, and the following observations shoul be emphasized: differences in the subtelomeric sequence signals; homologues (with different arm ratios) of chromosomes 2 and 4 formed normal bivalents at pachtytene. This aspect apparently provides evidence for epigenetic location of centromeres, a finding that has been observed in many organisms. MenosThe analysis of pachytene chromosomes has greatly contributed to maize cytogenetics. Recently, FISH procedures using repeated DNA sequences have been described and improved the identification of somatic chromosomes. Due to the polymorphism of knobs and repetitive DNA sequences, karyotype diversity is observed among varieties. Mitotic chromosome analysis is valuable for the screening of several individuals, and studies of evolution. We investigaded root-tip chromosomes of inbred lines and hybrids, derived from a tropical variety (Cateto races), using the following sequences as probes: subtelomeric 4-12-1 clone (PNAS 101, 13555), knob 180-bp sequence, 5S rDNA, centromeric satellite 4 (Cent 4). Pachytene chromosomes and C-banded mitotic metaphases were previously analysed, and except for chromosomes 2, 4 and 5, most chromosomes were distinguished by their length, arm ratio or presence of knobs. We identified chromosomes 2 and 4 using probes of 5S rDNA (2L) and Cent4, respectively, but their arm ratios were atypical. Signals of the 180-bp sequence were correspondent to knobs detected by C-banding, and additional small signals were found at 1S, 6S and 9S, located at arm tips, a feature also found in other varieties. The subtelomeric sequence was observed at 1S, 2S, 4SL, 5S, 8L. One line was crossed with KYS, an inbred extensively investigated, and the following observations shoul be emphasized: differences in the subtelomeric sequence signals; homologues (with different arm ratio... Mostrar Tudo |
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Palavras-Chave: |
Maize cytogenetics. |
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Thesaurus Nal: |
chromosomes. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02599nam a2200205 a 4500
001 1654675
005 2023-07-24
008 2007 bl uuuu u00u1 u #d
022 $a0967-3849
100 1 $aAGUIAR-PERECIN, M. L. R.
245 $aAnalysis of karyotype variability of tropical maize inbred lines and hybrids using FISH of tandemly repeated DNA sequences to identify somatic chromosomes.$h[electronic resource]
260 $aIn: Chomosome Research, Netherlands, v. 15, n. 2, p. 41 jul. 2007. Supplement. P.088$c2007
500 $aEdição do 16 INTERNATIONAL CHROMOSOME CONFERENCE, Abstracts...Amsterdam, ago. 2007. Editado por Hans de Jong, Hans Tanke and Paul Fransz. DOI: 10.1007/s10577 ISSN 0967-3849 em papel
520 $aThe analysis of pachytene chromosomes has greatly contributed to maize cytogenetics. Recently, FISH procedures using repeated DNA sequences have been described and improved the identification of somatic chromosomes. Due to the polymorphism of knobs and repetitive DNA sequences, karyotype diversity is observed among varieties. Mitotic chromosome analysis is valuable for the screening of several individuals, and studies of evolution. We investigaded root-tip chromosomes of inbred lines and hybrids, derived from a tropical variety (Cateto races), using the following sequences as probes: subtelomeric 4-12-1 clone (PNAS 101, 13555), knob 180-bp sequence, 5S rDNA, centromeric satellite 4 (Cent 4). Pachytene chromosomes and C-banded mitotic metaphases were previously analysed, and except for chromosomes 2, 4 and 5, most chromosomes were distinguished by their length, arm ratio or presence of knobs. We identified chromosomes 2 and 4 using probes of 5S rDNA (2L) and Cent4, respectively, but their arm ratios were atypical. Signals of the 180-bp sequence were correspondent to knobs detected by C-banding, and additional small signals were found at 1S, 6S and 9S, located at arm tips, a feature also found in other varieties. The subtelomeric sequence was observed at 1S, 2S, 4SL, 5S, 8L. One line was crossed with KYS, an inbred extensively investigated, and the following observations shoul be emphasized: differences in the subtelomeric sequence signals; homologues (with different arm ratios) of chromosomes 2 and 4 formed normal bivalents at pachtytene. This aspect apparently provides evidence for epigenetic location of centromeres, a finding that has been observed in many organisms.
650 $achromosomes
653 $aMaize cytogenetics
700 1 $aMONDIN, M.
700 1 $aSANTOS-SEREJO, J. A. dos
700 1 $aVIDA, A. C. F.
700 1 $aMOLINA, S. C. M.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 1. |  | SABINO, L. A.; SOUZA JÚNIOR, V. R.; ABREU, P. G. de; ABREU, V. M. N.; FARAH, M. M.; COLDEBELLA, A. Avaliação do ambiente de dois modelos de sala de maternidade na suinocultura com o uso do programa surfer. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 48., 2011, Belém. Anais... Belém: UFRA, 2011. 1 CD-ROM. Projeto: 11.11.11.111.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 2. | | SOUZA JÚNIOR, V. R.; ABREU, P. G. de; SABINO, L. A.; COLDEBELLA, A.; ABREU, V. M. N.; MEIRA, C. T. Frequência de conforto da temperatura e umidade em creches de suínos com programas de iluminação nos períodos de inverno e verão. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 48., 2011, Belém. Anais... Belém: UFRA, 2011. 1 CD-ROM. Projeto: 11.11.11.111.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 4. | | SOUZA JÚNIOR, V. R.; ABREU, P. G. de; LOPES, L. dos S.; COLDEBELLA, A.; FARAH, M. M.; SABINO, L. A. Influência da iluminação artificial no consumo de ração em leitões na fase de creche. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 47., 2010, Salvador. Empreendedorismo e progresso científicos na zootecnia brasileira de vanguarda: anais. Salvador: SBZ, 2010. 1 CD-ROM. Projeto/Plano de Ação: 03.07.94.900-04.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 6. | | SOUZA JÚNIOR, V. R.; ABREU, P. G. de; COLDEBELLA, A.; LOPES, L. dos S.; LIMA, G. J. M. M. de; SABINO, L. A. Iluminação artificial no desempenho de leitões na fase de creche. Acta Scientiarum. Animal Sciences, Maringá, v. 33, n. 4, p. 403-408, 2011. Projeto: 03.07.09.049.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 7. | | SOUZA JUNIOR, V. R. de; ABREU, P. G. de; COLDEBELLA, A.; FARAH, M. M.; LOPES, L. dos S.; SABINO, L. A. Influência da iluminação artificial no ganho de peso de leitões na fase de creche. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 47., 2010, Salvador. Empreendedorismo e progresso científicos na zootecnia brasileira de vanguarda: anais. Salvador: SBZ, 2010. 1 CD-ROM. Projeto/Plano de Ação: 03.07.94.900-04.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 8. | | COSTA, J. A. A. da; NEVES, A. P.; SILVEIRA, L. de S. M.; VILLAFUERTE, S. F. E.; GUIMARAES, R. L. S.; PROCIÚNCULA, G. C. da; SOUZA JUNIOR, V. R. de; VERZIGNASSI, J. R.; QUEIROZ, H. P. de. Consórcio de guandu com milho ou com sorgo para produção de silagem. Campo Grande, MT: Embrapa Gado de Corte, 2017. 16 p. (Embrapa Gado de Corte. Comunicado técnico, 143).| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 9. | | ABREU, P. G. de; LIMA, G. J. M. M. de; DALLA COSTA, O. A.; SOUZA JÚNIOR, V. R.; ABREU, V. M. N.; AJALA, L. C.; LOPES, L. dos S.; COLDEBELLA, A.; PALHARES, J. C. P.; MORES, N.; AMARAL, A. L. do; SANTOS FILHO, J. I. dos; SABINO, L. A. Efeito do programa de luz artificial, nas características térmicas ambientais, bem-estar, saúde e desempenho de suínos na fase de creche. In: COLDEBELLA, A.; SCHEUERMANN, G. N. (Ed.). Relatório de projetos concluídos 2011. Concórdia: Embrapa Suínos e Aves, 2012. p. 133-142. (Embrapa Suínos e Aves. Documentos, 156) Projeto/Plano de Ação: 03.07.09.049.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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