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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
23/03/2009 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, O. C. S.; BARROS, E. M.; BRITO, M. A. V. P.; BASTOS, M. C. F.; SANTOS, K. R. N.; GIAMBIAGI-DE-MARVAL, M. |
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Afiliação: |
Olinda Cabral da Silva Santos, UFRJ; Elaine Menezes Barros, UFRJ; Maria Aparecida Vasconcelos Paiva Brito, Embrapa Gado de Leite; Maria do Carmo de Freire Bastos, UFRJ; Kátia Regina Netto dos Santos, UFRJ; Marcia Giambiagi-deMarval, UFRJ. |
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Título: |
Identification of coagulase-negative staphylococci from bovine mastitis using RFLP-PCR of the groEL gene. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Veterinary Microbiology, n. 130, n. 1/2, p. 134-140, 2008. |
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DOI: |
https://doi.org/10.1016/j.vetmic.2007.12.009 |
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Idioma: |
Inglês |
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Conteúdo: |
Coagulase-negative staphylococci (CNS) have become the predominant pathogens causing bovine mastitis in many countries. CNS infections are associated with damage to milk secretory tissue of the mammary gland by increased connective tissue stroma, moderate increases of somatic cells count in milk and significant production decreases. These consequences impose serious economic losses for the farmers and the dairy industry. Routine veterinary laboratories do not usually identify CNS at the species level. Thereby, the aims of this study were to identify the most common staphylococcal pathogens involved in bovine mastitis using PCR-restriction fragment length polymorphism (RFLP) analysis of a partial groEL gene sequence and to compare our results with the identification carried out by the conventional method. A total of 54 isolates of Staphylococcus, involved in bovine mastitis, were analyzed by this method. The size and number of the fragments obtained by either AluI or HindIII/PvuII digestions made possible to form clear patterns differentiating, among the isolates, 11 of the most common species of animal staphylococcal pathogens. Most of the isolates clustered together with the reference strain of Staphylococcus chromogenes (28) and the type strain of Staphylococcus epidermidis (8). Besides, some isolates clustered together with the type strain of Staphylococcus aureus (5). All patterns were confirmed by the conventional biochemical method, showing concordant results. Thus, the PCR-RFLP of the groEL gene constitutes a reliable and reproducible molecular method for identification of CNS species responsible for bovine mastitis. MenosCoagulase-negative staphylococci (CNS) have become the predominant pathogens causing bovine mastitis in many countries. CNS infections are associated with damage to milk secretory tissue of the mammary gland by increased connective tissue stroma, moderate increases of somatic cells count in milk and significant production decreases. These consequences impose serious economic losses for the farmers and the dairy industry. Routine veterinary laboratories do not usually identify CNS at the species level. Thereby, the aims of this study were to identify the most common staphylococcal pathogens involved in bovine mastitis using PCR-restriction fragment length polymorphism (RFLP) analysis of a partial groEL gene sequence and to compare our results with the identification carried out by the conventional method. A total of 54 isolates of Staphylococcus, involved in bovine mastitis, were analyzed by this method. The size and number of the fragments obtained by either AluI or HindIII/PvuII digestions made possible to form clear patterns differentiating, among the isolates, 11 of the most common species of animal staphylococcal pathogens. Most of the isolates clustered together with the reference strain of Staphylococcus chromogenes (28) and the type strain of Staphylococcus epidermidis (8). Besides, some isolates clustered together with the type strain of Staphylococcus aureus (5). All patterns were confirmed by the conventional biochemical method, showing concordant results. Thus, th... Mostrar Tudo |
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Palavras-Chave: |
Coagulase-negative staphylococci; groEL gene. |
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Thesaurus Nal: |
bovine mastitis; Staphylococcus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02440naa a2200241 a 4500
001 1596414
005 2024-02-05
008 2008 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.vetmic.2007.12.009$2DOI
100 1 $aSANTOS, O. C. S.
245 $aIdentification of coagulase-negative staphylococci from bovine mastitis using RFLP-PCR of the groEL gene.$h[electronic resource]
260 $c2008
520 $aCoagulase-negative staphylococci (CNS) have become the predominant pathogens causing bovine mastitis in many countries. CNS infections are associated with damage to milk secretory tissue of the mammary gland by increased connective tissue stroma, moderate increases of somatic cells count in milk and significant production decreases. These consequences impose serious economic losses for the farmers and the dairy industry. Routine veterinary laboratories do not usually identify CNS at the species level. Thereby, the aims of this study were to identify the most common staphylococcal pathogens involved in bovine mastitis using PCR-restriction fragment length polymorphism (RFLP) analysis of a partial groEL gene sequence and to compare our results with the identification carried out by the conventional method. A total of 54 isolates of Staphylococcus, involved in bovine mastitis, were analyzed by this method. The size and number of the fragments obtained by either AluI or HindIII/PvuII digestions made possible to form clear patterns differentiating, among the isolates, 11 of the most common species of animal staphylococcal pathogens. Most of the isolates clustered together with the reference strain of Staphylococcus chromogenes (28) and the type strain of Staphylococcus epidermidis (8). Besides, some isolates clustered together with the type strain of Staphylococcus aureus (5). All patterns were confirmed by the conventional biochemical method, showing concordant results. Thus, the PCR-RFLP of the groEL gene constitutes a reliable and reproducible molecular method for identification of CNS species responsible for bovine mastitis.
650 $abovine mastitis
650 $aStaphylococcus
653 $aCoagulase-negative staphylococci
653 $agroEL gene
700 1 $aBARROS, E. M.
700 1 $aBRITO, M. A. V. P.
700 1 $aBASTOS, M. C. F.
700 1 $aSANTOS, K. R. N.
700 1 $aGIAMBIAGI-DE-MARVAL, M.
773 $tVeterinary Microbiology$gn. 130, n. 1/2, p. 134-140, 2008.
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| Registros recuperados : 22 | |
| 3. |  | SOUSA, C. T. de; MACHADO, T. F.; NOGUEIRA, N. A. P.; LIMA, E. C. de. Avaliação da atividade antimicrobiana do óleo essencial de Lippia alba frente ao Staphylococcus aureus, à Salmonella choleraesuis, Echerichia coli e Pseudomona aeruginosa. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AGROINDÚSTRIA TROPICAL, 8., 2010, Fortaleza. Resumos. Fortaleza: Embrapa Agroindústria Tropical, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 9. | | MACHADO, T. F.; NOGUEIRA, N. A. P.; PEREIRA, R. de C. A.; SILVA, M. G. V.; SOUSA, C. T. de. Atividade antimicrobiana de óleos essenciais de diferentes espécies de Ocimum. In: CONGRESO LATINOAMERICANO DE MICROBIOLOGÍA E HIGIENE DE ALIMENTOS, 10., 2009, Punta del Este, Uruguai: International Commission on Microbiological Specifications for Foods, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 14. | | MACHADO, T. F.; NOGUEIRA, N. A. P.; PEREIRA, R. de C. A.; SOUSA, C. T. de; BATISTA, V. C. V. The antimicrobial efficacy of Lippia alba essential oil and its interaction with food ingredients. Brazilian Journal of Microbiology, São Paulo, v. 45, n. 2, p. 699-705, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
|  |
| 17. | | MACHADO, T. F.; BORGES, M. de F.; PORTO, B. de C.; SOUSA, C. T. de; OLIVEIRA, F. E. M. de. Interferência da microbiota autóctone do queijo coalho sobre Staphylococcus coagulase positiva. Revista Ciência Agronômica, Fortaleza, v. 42, n. 2, p. 337-341, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 18. | | SOUSA, C. T. de; MACHADO, T. F.; NOGUEIRA, N. A. P.; PEREIRA, R. de C. A.; SILVA, M. G. V.; LIMA, D. B. Atividade antimicrobiana de extratos hidroalcoólicos de espécies de Ocimum. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AGROINDÚSTRIA TROPICAL, 7., 2009, Fortaleza. Resumos. Fortaleza: Embrapa Agroindústria Tropical, 2009. p. 20.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 19. | | MACHADO, T. F.; BRUNO, L. M.; BORGES, M. de F.; OLIVEIRA, F. E. M. de; PORTO, B. C.; SOUSA, C. T. de. Isolamento e caracterização bioquímica de Salmonella spp. em amostras de queijo coalho. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 1., 2010, Salvador. Anais. Salvador: Sociedade Brasileira de Recursos Genéticos, 2010. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 20. | | FEITOSA, T.; BORGES, M. de F.; ANDRADE, A. P. C. de; PORTO, B. C.; SOUSA, C. T. de; BENTO, G. T. Coleção de microrganismos patógenos da Embrapa Agroindústria Tropical. In: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasilia,DF. Resumos... Brasilia, DF: Embrapa Reursos Genéticos e Biotecnologia: FUNCREDI, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| Registros recuperados : 22 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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