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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
12/03/2004 |
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Data da última atualização: |
28/08/2023 |
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Autoria: |
MAXWELL, W. M. C.; EVANS, G.; MORTIMER, S. T.; GILLAN, L.; GELLATLY, E. S.; McPHIE, C. A. |
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Título: |
Normal fertility in ewes after cervical insemination with frozen-thawed spermatozoa supplemented with seminal plasma. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 11, n. 2, p. 123-126, 1999. |
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DOI: |
10.1071/rd99046. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The effect of seminal plasma (SP) on the motility, capacitation status, penetration through cervical mucus and fertility of frozen-thawed ram spermatozoa was examined. ln the presence of SP, motility of frozen-thawed spermatozoa was better (P<0.001) and there were more uncapacitated and less acrosomereacted cells in comparison with controls (P<0.001). Frozen-thawed spermatozoa were also better able to penetrate cervical mucus after addition of SP. Addition of SP increased the percentage of ewes pregnant after insemination offrozen-thawed (39/94, 41.5% v. 51/92,55.4%; P<0.05) but not fresh spermatozoa (34/55, 61.8% v. 42/58,72.4% for 0 v. 30% SP in the resuspension medium). Moreover, SP improved pregnancy rates after cervical (14/50; 28% v. 25/49; 51%; P<0.05) but not intrauterine insemination (25/44; 56.8 v. 26/43; 60.5%) with frozen-thawed spermatozoa. ln a second experiment, pregnancy rates were 30/45 (66.7%), 9/37 (24.3%) and 24/40 (60.0%) for ewes inseminated with frozen-thawed spermatozoa in the uterus (control), cervix without SP and cervix after supplementation with SP, respectively (P<0.01 for unsupplemented v. supplemented spermatozoa). These experiments demonstrate that impaired function of cryopreserved spermatozoa can be overcome by addition of SP. resulting in normal fertility after cervical AI. |
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Palavras-Chave: |
Cervix Uteri; Semen Preservation; Sperm-Ovum Interactions. |
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Thesaurus Nal: |
Cryopreservation; Physiology; Pregnancy; Reproduction; Sheep; Sperm capacitation; Sperm motility; Spermatozoa. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02308naa a2200325 a 4500
001 1530358
005 2023-08-28
008 1999 bl uuuu u00u1 u #d
024 7 $a10.1071/rd99046.$2DOI
100 1 $aMAXWELL, W. M. C.
245 $aNormal fertility in ewes after cervical insemination with frozen-thawed spermatozoa supplemented with seminal plasma.$h[electronic resource]
260 $c1999
520 $aAbstract: The effect of seminal plasma (SP) on the motility, capacitation status, penetration through cervical mucus and fertility of frozen-thawed ram spermatozoa was examined. ln the presence of SP, motility of frozen-thawed spermatozoa was better (P<0.001) and there were more uncapacitated and less acrosomereacted cells in comparison with controls (P<0.001). Frozen-thawed spermatozoa were also better able to penetrate cervical mucus after addition of SP. Addition of SP increased the percentage of ewes pregnant after insemination offrozen-thawed (39/94, 41.5% v. 51/92,55.4%; P<0.05) but not fresh spermatozoa (34/55, 61.8% v. 42/58,72.4% for 0 v. 30% SP in the resuspension medium). Moreover, SP improved pregnancy rates after cervical (14/50; 28% v. 25/49; 51%; P<0.05) but not intrauterine insemination (25/44; 56.8 v. 26/43; 60.5%) with frozen-thawed spermatozoa. ln a second experiment, pregnancy rates were 30/45 (66.7%), 9/37 (24.3%) and 24/40 (60.0%) for ewes inseminated with frozen-thawed spermatozoa in the uterus (control), cervix without SP and cervix after supplementation with SP, respectively (P<0.01 for unsupplemented v. supplemented spermatozoa). These experiments demonstrate that impaired function of cryopreserved spermatozoa can be overcome by addition of SP. resulting in normal fertility after cervical AI.
650 $aCryopreservation
650 $aPhysiology
650 $aPregnancy
650 $aReproduction
650 $aSheep
650 $aSperm capacitation
650 $aSperm motility
650 $aSpermatozoa
653 $aCervix Uteri
653 $aSemen Preservation
653 $aSperm-Ovum Interactions
700 1 $aEVANS, G.
700 1 $aMORTIMER, S. T.
700 1 $aGILLAN, L.
700 1 $aGELLATLY, E. S.
700 1 $aMcPHIE, C. A.
773 $tReproduction, Fertility and Development$gv. 11, n. 2, p. 123-126, 1999.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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