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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/06/1998 |
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Data da última atualização: |
12/06/2018 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
LOPES, M. A.; LARKINS, B. A. |
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Afiliação: |
EMBRAPA-CNPMS. |
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Título: |
Genetic analysis of endosperm modification in quality protein maize. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON QUALITY PROTEIN MAIZE, 1994, Sete Lagoas. Quality protein maize: 1964-1994: proceedings. [S.l.]: Purdue University, 1997. p. 149-173. |
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Idioma: |
Inglês |
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Conteúdo: |
A combination of genetic and biochemical analyses were used to investigate the number and mode of action of opaque-2 (o2) modifier genes and possible associations between seed modification and endosperm protein accumulation. Genetic analysis indicated the existence of two additive modifier genes in the populations studied. Analysis of 02, modified 02, their reciprocal F1's, and segregating progenies indicated that increased deposition of gamma zein, a cysteine-rich storage protein that contains no lysine, is dependent on the dosage of 02 modifier genes and directly correlated to seed modification. Molecular mapping by RFLP and Bulked Segregant Analyisis (BSA) indicated that a modifier gene maps to the same chromosomal region where gamma-zein genes are located. Analysis of recombination inbred lines with variable degrees of seed modification showed that allelic composition at the gamma-zein locus correlated with variations in endosperm modification. When we consider the possible involvement of gamma-zein in QPM endosperm modification, it is difficult to imagine that selection for increased seed modification would cause increased accumulation of a lysine-poor protein unrelated to the modification process, thus antagonizing efforts to maintain high nutritional quality. This is especially true because the selection procedures used for o2 modification placed a high priority on maintenance of seed nutritional quality. Therefore, it appears that increased accumulation of the lysine-poor gamma-zein protein was a necessary route to alter seed phenotype. Apparently, the cost of this process was a slight reduction of seed nutritional quality relative to the unmodified 02, due to increased gamma-zein accumulation. Although non-zein (lysine-containing proteins) content was found to be negatively correlated with seed density, amount of gamma-zein, and amount of total zein in the endosperm, we found that it is possible to select QPM lines with high levels of seed modification and high non-zein content. These results indicate the potential for further improvements in QPM nutritional quality. MenosA combination of genetic and biochemical analyses were used to investigate the number and mode of action of opaque-2 (o2) modifier genes and possible associations between seed modification and endosperm protein accumulation. Genetic analysis indicated the existence of two additive modifier genes in the populations studied. Analysis of 02, modified 02, their reciprocal F1's, and segregating progenies indicated that increased deposition of gamma zein, a cysteine-rich storage protein that contains no lysine, is dependent on the dosage of 02 modifier genes and directly correlated to seed modification. Molecular mapping by RFLP and Bulked Segregant Analyisis (BSA) indicated that a modifier gene maps to the same chromosomal region where gamma-zein genes are located. Analysis of recombination inbred lines with variable degrees of seed modification showed that allelic composition at the gamma-zein locus correlated with variations in endosperm modification. When we consider the possible involvement of gamma-zein in QPM endosperm modification, it is difficult to imagine that selection for increased seed modification would cause increased accumulation of a lysine-poor protein unrelated to the modification process, thus antagonizing efforts to maintain high nutritional quality. This is especially true because the selection procedures used for o2 modification placed a high priority on maintenance of seed nutritional quality. Therefore, it appears that increased accumulation of the lysine... Mostrar Tudo |
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Palavras-Chave: |
Maize; Protein; Quality. |
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Thesagro: |
Biologia Molecular; Milho; Qualidade; Zea Mays. |
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Thesaurus Nal: |
molecular biology. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02818nam a2200217 a 4500
001 1479111
005 2018-06-12
008 1997 bl uuuu u00u1 u #d
100 1 $aLOPES, M. A.
245 $aGenetic analysis of endosperm modification in quality protein maize.$h[electronic resource]
260 $aIn: INTERNATIONAL SYMPOSIUM ON QUALITY PROTEIN MAIZE, 1994, Sete Lagoas. Quality protein maize: 1964-1994: proceedings. [S.l.]: Purdue University, 1997. p. 149-173.$c1997
520 $aA combination of genetic and biochemical analyses were used to investigate the number and mode of action of opaque-2 (o2) modifier genes and possible associations between seed modification and endosperm protein accumulation. Genetic analysis indicated the existence of two additive modifier genes in the populations studied. Analysis of 02, modified 02, their reciprocal F1's, and segregating progenies indicated that increased deposition of gamma zein, a cysteine-rich storage protein that contains no lysine, is dependent on the dosage of 02 modifier genes and directly correlated to seed modification. Molecular mapping by RFLP and Bulked Segregant Analyisis (BSA) indicated that a modifier gene maps to the same chromosomal region where gamma-zein genes are located. Analysis of recombination inbred lines with variable degrees of seed modification showed that allelic composition at the gamma-zein locus correlated with variations in endosperm modification. When we consider the possible involvement of gamma-zein in QPM endosperm modification, it is difficult to imagine that selection for increased seed modification would cause increased accumulation of a lysine-poor protein unrelated to the modification process, thus antagonizing efforts to maintain high nutritional quality. This is especially true because the selection procedures used for o2 modification placed a high priority on maintenance of seed nutritional quality. Therefore, it appears that increased accumulation of the lysine-poor gamma-zein protein was a necessary route to alter seed phenotype. Apparently, the cost of this process was a slight reduction of seed nutritional quality relative to the unmodified 02, due to increased gamma-zein accumulation. Although non-zein (lysine-containing proteins) content was found to be negatively correlated with seed density, amount of gamma-zein, and amount of total zein in the endosperm, we found that it is possible to select QPM lines with high levels of seed modification and high non-zein content. These results indicate the potential for further improvements in QPM nutritional quality.
650 $amolecular biology
650 $aBiologia Molecular
650 $aMilho
650 $aQualidade
650 $aZea Mays
653 $aMaize
653 $aProtein
653 $aQuality
700 1 $aLARKINS, B. A.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 6 | |
| 1. |  | FREIRE FILHO, F. R.; ROCHA, M. M.; RIBEIRO, V. Q.; SILVA, K. J. D.; LOPES, A. M.; CRAVO, M. S.; CARVALHO, H. W. L.; VILARINHO, A. A.; SABOYA, R. C. C.; CAVALCANTE, E. S.; COSTA, A. F.; ALCANTARA, J. P.; SITTOLIN, I. M.; NUTTI, M. R.; CARVALHO, J. L. V. BRS Aracê: cultivar de feijão-caupi com grãos de cor verde oliva rica em ferro e zinco. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 2., 2009, Belém, PA. Da agricultura de subsitência ao agronegócio: anais. Belém, PA: Embrapa Amazônia Oriental, 2009. p. 339-343. 1 CD-ROM. Publicado também como folder (00705).| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amapá; Embrapa Amazônia Oriental; Embrapa Meio-Norte; Embrapa Roraima; Embrapa Tabuleiros Costeiros. |
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| 2. | | FREIRE FILHO, F. R.; ROCHA, M. M.; RIBEIRO, V. Q.; SILVA, K. J. D.; LOPES, A. M.; CRAVO, M. S.; CARVALHO, H. W. L.; VILARINHO, A. A.; SABOYA, R. C.; CAVALCANTE, E. S.; COSTA, A. F.; ALCANTARA, J. P.; SITTOLIN, I. M.; NUTTI, M. R.; CARVALHO, J. L. V. BRS Itaim: cultivar de feijão-caupi com grãos do tipo fradinho. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 2., 2009, Belém, PA. Da agricultura de subsitência ao agronegócio: anais. Belém, PA: Embrapa Amazônia Oriental, 2009. p. 344-348. 1 CD-ROM. Publicado também como folder (00706).| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Meio-Norte; Embrapa Roraima; Embrapa Tabuleiros Costeiros. |
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| 3. | | FREIRE FILHO, F. R.; ROCHA, M. M.; RIBEIRO, V. Q.; SILVA, K. J. D.; LOPES, A. M.; CRAVO, M. S.; CARVALHO, H. W. L.; VILARINHO, A. A.; SABOYA, R. C.; CAVALCANTE, E. S.; COSTA, A. F.; ALCANTARA, J. P.; SITTOLIN, I. M.; NUTTI, M. R.; CARVALHO, J. L. V. BRS Juruá: cultivar de feijão-caupi com grãos de cor verde persistente rica em ferro e zinco. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 2., 2009, Belém, PA. Da agricultura de subsitência ao agronegócio: anais. Belém, PA: Embrapa Amazônia Oriental, 2009. p. 349-353. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Roraima; Embrapa Tabuleiros Costeiros. |
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| 4. | | FREIRE FILHO, F. R.; CRAVO, M. da S.; VILARINHO, A. A.; CAVALCANTE, E. da S.; FERNANDES, J. B.; SAGRILO, E.; RIBEIRO, V. Q.; ROCHA, M. de M.; SITTOLIN, I. M.; SOUZA, F. de F.; LOPES, A. de M.; ROCHA NETO, O. G. da; CELESTINO FILHO, P.; GONCALVES, J. R. P.; CARVALHO, H. W. L. de; RAPOSO, J. A. A.; SAMPAIO, L. S. BRS NOVAERA: cultivar de feijão-caupi de porte semi-ereto. Belém, PA: Embrapa Amazônia Oriental; Teresina: Embrapa Meio-Norte, 2007. Não paginado, il. 1 folder. Equipe Técnica: Francisco Rodrigues Freire Filho, Manoel da Silva Cravo, Aluísio Alcântara Vilarinho, Emanuel da Silva Cavalcante, João Batista Fernandes, Edvaldo Sagrilo, Valdenir Queiroz Ribeiro, Maurisrael de Moura Rocha,...| Tipo: Folder/Folheto/Cartilha |
| Biblioteca(s): Embrapa Amapá; Embrapa Amazônia Oriental; Embrapa Rondônia. |
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| 5. | | JESÚS-PIRES, C. de; FERREIRA-NETO, J. R. C.; BEZERRA-NETO, J. P.; KIDO, E. A.; SILVA, R. L. de O.; PANDOLFI, V.; WANDERLEY-NOGUEIRA, A. C.; BINNECK, E.; COSTA, A. F. da; PIO-RIBEIRO, G.; PEREIRA-ANDRADE, G.; SITTOLIN, I. M.; FREIRE-FILHO, F.; BENKO-ISEPPON, A. M. Plant Thaumatin-like Proteins: Function, Evolution and Biotechnological Applications. Current Protein and Peptide Science, v. 21, n. 1, p. 36-51, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Soja. |
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| 6. | | FREIRE FILHO, F. R.; ROCHA, M. de M.; RIBEIRO, V. Q.; SITTOLIN, I. M.; CARVALHO, H. W. L. de; COSTA, A. F. de; ALCÂNTARA, J. dos P.; FERNANDES, J. B.; GONÇALVES, J. R. P.; VILARINHO, A. A.; CRAVO, M. da S.; CAVALCANTE, E. da S.; NUTTI, M. R. BRS Xiquexique: cultivar de feijão-caupi rica em ferro e zinco. Teresina: Embrapa Meio-Norte, 2008. 4 p. (Embrapa Meio-Norte. Comunicado Técnico, 209).| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Meio-Norte; Embrapa Roraima. |
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| Registros recuperados : 6 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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