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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
16/07/2009 |
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Data da última atualização: |
29/05/2025 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
KANAMORI, N.; GIROTTO, L.; SANCHES, B. B.; LEITE, J. P.; ENGELS, C.; ROLLA, A. A. P.; FUGANTI, R.; NEUMAIER, N.; FARIAS, J. R. B.; ABDELNOOR, R. V.; MARCELINO, F. C.; NEPOMUCENO, A. L. |
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Afiliação: |
N. KANAMORI, JIRCAS; AMANDA A. PAIVA ROLLA; RENATA FUGANTI; NORMAN NEUMAIER, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; FRANCISMAR CORREA MARCELINO, CNPSO; ALEXANDRE LIMA NEPOMUCENO, CNPSO. |
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Título: |
Agrobacterium-mediated transformation of brazilian soybean cultivar, BR 16. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE SOJA, 5.; MERCOSOJA 2009, Goiânia. Anais... Londrina: Embrapa Soja, 2009. Seção trabalhos, t. 177. 1 CD-ROM. Editado por Adilson de Oliveira Júnior, Odilon Ferreira Saraiva, Clara Beatriz Hoffmann Campo, César de Castro. |
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Idioma: |
Inglês |
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Conteúdo: |
Soybean Glycine max (L.) Merril.have proven to be extremely resistant to transformation. In Brazil, transformation of soybean had succeeded using particle bombardment (bio-ballistic). In this study, soybean transformation using Agrobacterium was optimized. Mature soybean seeds were soaked for 16h and the embryonic tip, cotyledonary node and hypocotyl segment were collected. These explants were inoculated with Agrobacterium tumefaciens strain EHA105, which contains the construction pGreenII0229 integrated with 35S:GUS:NOS cassette. The use of embryonic tips yielded a higher transformation frequency when compared to cotyledonary nodes and hypocotyls. Results showed that T-DNA transfer efficiency reached to approximately 80%, and transformed shoot primordia and leaf primordia were observed on shoot elongation medium. |
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Thesagro: |
Agrobacterium Tumefaciens; Biotecnologia; Soja. |
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Thesaurus Nal: |
Soybeans. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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100 1 $aKANAMORI, N.
245 $aAgrobacterium-mediated transformation of brazilian soybean cultivar, BR 16.
260 $c2009
520 $aSoybean Glycine max (L.) Merril.have proven to be extremely resistant to transformation. In Brazil, transformation of soybean had succeeded using particle bombardment (bio-ballistic). In this study, soybean transformation using Agrobacterium was optimized. Mature soybean seeds were soaked for 16h and the embryonic tip, cotyledonary node and hypocotyl segment were collected. These explants were inoculated with Agrobacterium tumefaciens strain EHA105, which contains the construction pGreenII0229 integrated with 35S:GUS:NOS cassette. The use of embryonic tips yielded a higher transformation frequency when compared to cotyledonary nodes and hypocotyls. Results showed that T-DNA transfer efficiency reached to approximately 80%, and transformed shoot primordia and leaf primordia were observed on shoot elongation medium.
650 $aSoybeans
650 $aAgrobacterium Tumefaciens
650 $aBiotecnologia
650 $aSoja
700 1 $aGIROTTO, L.
700 1 $aSANCHES, B. B.
700 1 $aLEITE, J. P.
700 1 $aENGELS, C.
700 1 $aROLLA, A. A. P.
700 1 $aFUGANTI, R.
700 1 $aNEUMAIER, N.
700 1 $aFARIAS, J. R. B.
700 1 $aABDELNOOR, R. V.
700 1 $aMARCELINO, F. C.
700 1 $aNEPOMUCENO, A. L.
773 $tIn: CONGRESSO BRASILEIRO DE SOJA, 5.; MERCOSOJA 2009, Goiânia. Anais... Londrina: Embrapa Soja, 2009. Seção trabalhos, t. 177. 1 CD-ROM. Editado por Adilson de Oliveira Júnior, Odilon Ferreira Saraiva, Clara Beatriz Hoffmann Campo, César de Castro.
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Registro original: |
Embrapa Soja (CNPSO) |
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