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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
01/10/2002 |
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Data da última atualização: |
08/11/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMOS, H. J. O.; RONCATO-MACCARI, L.D.B.; SOUZA, E. M.; SOARES-RAMOS, J.R.L.; HUNGRIA, M.; PEDROSA, F. O. |
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Afiliação: |
MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Monitoring Azospirillum-wheat interactions using the gfp and gusA genes constitutively expressed from a new broad-host range vector. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Journal of Biotechnology, v. 97, n. 3, p. 243-252, Aug. 2002. |
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DOI: |
10.1016/S0168-1656(02)00108-6 |
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Idioma: |
Inglês |
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Conteúdo: |
To monitor the colonization of wheat roots by Azospirillum brasilense, we constructed several plasmids based on the pBBR1replicon expressing the gfp and gusA genes constitutively. Both genes were placed under control of the gentamycin resistance gene promoter resulting in high levels of expression in Escherichia coli and A. brasilense. The constructed plasmids were stably maintained in A. brasilense strains even in the absence of selective pressure. The colonization of wheat plants grown under controlled conditions in sterilized vermiculite by A. brasilense strain FP2 (aSp7-derivative) transconjugants containing these plasmids was monitored. Bacteria expressing GFP were easily observed in fresh plant material by fluorescence microscopy. Cell aggregates and single bacteria were visualized on the surfaces of young root zones, such as roots hairs and lateral roots. Large cellular clumps were observed at the points of lateral root emergence or at intercellular spaces of root epidermal cells 30 days after inoculation. Although we failed to detected bacteria in internal cortical and xylem tissues of wheat roots, the initial stage of endophytic colonization by A. brasilense may involve the sites detected in this work. |
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Thesagro: |
Trigo. |
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Thesaurus Nal: |
Azospirillum; Wheat. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01961naa a2200229 a 4500
001 1463274
005 2024-11-08
008 2002 bl uuuu u00u1 u #d
024 7 $a10.1016/S0168-1656(02)00108-6$2DOI
100 1 $aRAMOS, H. J. O.
245 $aMonitoring Azospirillum-wheat interactions using the gfp and gusA genes constitutively expressed from a new broad-host range vector.$h[electronic resource]
260 $c2002
520 $aTo monitor the colonization of wheat roots by Azospirillum brasilense, we constructed several plasmids based on the pBBR1replicon expressing the gfp and gusA genes constitutively. Both genes were placed under control of the gentamycin resistance gene promoter resulting in high levels of expression in Escherichia coli and A. brasilense. The constructed plasmids were stably maintained in A. brasilense strains even in the absence of selective pressure. The colonization of wheat plants grown under controlled conditions in sterilized vermiculite by A. brasilense strain FP2 (aSp7-derivative) transconjugants containing these plasmids was monitored. Bacteria expressing GFP were easily observed in fresh plant material by fluorescence microscopy. Cell aggregates and single bacteria were visualized on the surfaces of young root zones, such as roots hairs and lateral roots. Large cellular clumps were observed at the points of lateral root emergence or at intercellular spaces of root epidermal cells 30 days after inoculation. Although we failed to detected bacteria in internal cortical and xylem tissues of wheat roots, the initial stage of endophytic colonization by A. brasilense may involve the sites detected in this work.
650 $aAzospirillum
650 $aWheat
650 $aTrigo
700 1 $aRONCATO-MACCARI, L.D.B.
700 1 $aSOUZA, E. M.
700 1 $aSOARES-RAMOS, J.R.L.
700 1 $aHUNGRIA, M.
700 1 $aPEDROSA, F. O.
773 $tJournal of Biotechnology$gv. 97, n. 3, p. 243-252, Aug. 2002.
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Registro original: |
Embrapa Soja (CNPSO) |
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