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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
13/05/2025 |
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Data da última atualização: |
14/05/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SCHMID, A. P. P.; BRANDÃO, F. Z.; SANTOS, J. D. R.; LAEBER, C. C. R.; SILVA, S. R. da; KNUST, N. D.; BATISTA, R. I. T. P.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; BALARO, M. F. A.; FONSECA, J. F. da; UNGERFELD, R. |
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Afiliação: |
ANA PAULA PEREIRA SCHMID, UNIVERSIDADE FEDERAL FLUMINENSE; FELIPE ZANDONADI BRANDÃO, UNIVERSIDADE FEDERAL FLUMINENSE; JULIANA DANTAS RODRIGUES SANTOS, UNIVERSIDADE FEDERAL FLUMINENSE; CAMILA CORREA ROZA LAEBER, UNIVERSIDADE FEDERAL FLUMINENSE; SANDRA RIBEIRO DA SILVA, UNIVERSIDADE FEDERAL FLUMINENSE; NATHÁLIA DUTRA KNUST, UNIVERSIDADE FEDERAL FLUMINENSE; RIBRIO IVAN TAVARES PEREIRA BATISTA, UNIVERSIDADE FEDERAL FLUMINENSE; MARIA EMÍLIA FRANCO OLIVEIRA, UNIVERSIDADE FEDERAL FLUMINENSE; JOANNA MARIA G. SOUZA-FABJAN, UNIVERSIDADE FEDERAL FLUMINENSE; MÁRIO FELIPE ALVAREZ BALARO, UNIVERSIDADE FEDERAL FLUMINENSE; JEFERSON FERREIRA DA FONSECA, CNPC; RODOLFO UNGERFELD, UNIVERSIDAD DE LA REPÚBLICA. |
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Título: |
Biostimulation with rams improved embryo quality without modifying the ovulatory response in superovulated ewes. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Theriogenology, v. 243, 117463, Sept. 2025. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2025.117463 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The aim of this study was to evaluate whether extended ram exposure during FSH superovulatory treatment improves follicular growth, embryo production, and embryo quality in ewes. Twenty Santa Inês ewes were subjected to a superovulation protocol based on follicle-stimulating hormone (FSH) administration with or without male biostimulation in a crossover arrangement (n = 20 per treatment). Follicular development and corpus luteum functionality were monitored with ultrasound, and embryo collection was performed non-surgically. Recovered embryos were analyzed for gene expression, reactive oxygen species (ROS) levels, and blastomere count. Male biostimulation for 72 h did not modify the number or diameter of antral follicles, the recovery rate of structures, reactive oxygen species (ROS) levels, and blastomere count. However, it reduced the expression of genes linked to oxidative stress (PRDX1) and apoptosis (BCL2) in embryos. In summary, exposure to rams for 72 h during superovulatory treatment influenced molecular indicators of development competence, specifically the relative abundance of BCL2 and PRDX1 transcripts, which are associated with cellular apoptosis and stress. However, it did not improve the ovarian responses. |
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Palavras-Chave: |
Efeito macho; Embryo collection; FSH; MOET. |
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Thesagro: |
Biotecnologia; Embrião Animal; Ovelha; Ovino; Reprodução; Sincronização do Cio; Superovulação; Transferência de Embrião. |
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Thesaurus Nal: |
Biotechnology; Embryo (animal); Embryo transfer; Estrus synchronization; Ewes; Follicle-stimulating hormone; Ovarian follicles; Ram effect; Reproduction; Sheep; Superovulation; Teasing (breeding). |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02828naa a2200553 a 4500
001 2175547
005 2025-05-14
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2025.117463$2DOI
100 1 $aSCHMID, A. P. P.
245 $aBiostimulation with rams improved embryo quality without modifying the ovulatory response in superovulated ewes.$h[electronic resource]
260 $c2025
520 $aAbstract: The aim of this study was to evaluate whether extended ram exposure during FSH superovulatory treatment improves follicular growth, embryo production, and embryo quality in ewes. Twenty Santa Inês ewes were subjected to a superovulation protocol based on follicle-stimulating hormone (FSH) administration with or without male biostimulation in a crossover arrangement (n = 20 per treatment). Follicular development and corpus luteum functionality were monitored with ultrasound, and embryo collection was performed non-surgically. Recovered embryos were analyzed for gene expression, reactive oxygen species (ROS) levels, and blastomere count. Male biostimulation for 72 h did not modify the number or diameter of antral follicles, the recovery rate of structures, reactive oxygen species (ROS) levels, and blastomere count. However, it reduced the expression of genes linked to oxidative stress (PRDX1) and apoptosis (BCL2) in embryos. In summary, exposure to rams for 72 h during superovulatory treatment influenced molecular indicators of development competence, specifically the relative abundance of BCL2 and PRDX1 transcripts, which are associated with cellular apoptosis and stress. However, it did not improve the ovarian responses.
650 $aBiotechnology
650 $aEmbryo (animal)
650 $aEmbryo transfer
650 $aEstrus synchronization
650 $aEwes
650 $aFollicle-stimulating hormone
650 $aOvarian follicles
650 $aRam effect
650 $aReproduction
650 $aSheep
650 $aSuperovulation
650 $aTeasing (breeding)
650 $aBiotecnologia
650 $aEmbrião Animal
650 $aOvelha
650 $aOvino
650 $aReprodução
650 $aSincronização do Cio
650 $aSuperovulação
650 $aTransferência de Embrião
653 $aEfeito macho
653 $aEmbryo collection
653 $aFSH
653 $aMOET
700 1 $aBRANDÃO, F. Z.
700 1 $aSANTOS, J. D. R.
700 1 $aLAEBER, C. C. R.
700 1 $aSILVA, S. R. da
700 1 $aKNUST, N. D.
700 1 $aBATISTA, R. I. T. P.
700 1 $aOLIVEIRA, M. E. F.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aBALARO, M. F. A.
700 1 $aFONSECA, J. F. da
700 1 $aUNGERFELD, R.
773 $tTheriogenology$gv. 243, 117463, Sept. 2025.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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