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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sul. Para informações adicionais entre em contato com cppsul.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
01/09/2021 |
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Data da última atualização: |
01/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COSTA, R. J. da; SILVA, A. P. da; FONSECA, R. N. da; HÜBNER, S. de O.; NALERIO, E. S.; MARQUES, J. de L.; VITOLA, H. R. S.; SILVA, W. P. da; DUVAL, E. H.; FIORENTINI, A. M. |
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Afiliação: |
ROGER JUNGES DA COSTA, UFPEL; ANDRESA PEREIRA DA SILVA, IFSUL; RENATA NOBRE DA FONSECA, UFPEL; SILVIA DE OLIVEIRA HÜBNER, UFPEL; ELEN SILVEIRA NALERIO, CPPSUL; JULIANA DE LIMA MARQUES, UFPEL; HELENA REISSIG SOARES VITOLA, UFPEL; WLADIMIR PADILHA DA SILVA, UFPEL; EDUARDA HALLAL DUVAL, UFPEL; ANGELA MARIA FIORENTINI, UFPEL. |
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Título: |
Characterization of Enterococcus faecium EO1 isolated from mutton and activity of bacteriocin-like substances in the control of Listeria monocytogenes in fresh mutton sausage. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Food Science and Technology, v. 141, 110954, Apr. 2021. |
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DOI: |
https://doi.org/10.1016/j.lwt.2021.110954 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to isolate and characterize bacteriocinogenic lactic acid bacteria (LAB) from mutton carcasses after slaughter. The study also evaluated the activity of bacteriocin-like substances (BLS) for control of Listeria monocytogenes in fresh mutton sausage. The cell-free supernatant (CFS) was obtained and its antimicrobial activity was quantified. The presence of genes encoding enterocin was evaluated by molecular techniques. Another aspect assessed was the action in situ of BLS produced by these isolates for control of Listeria monocytogenes in fresh mutton sausage, experimentally contaminated. Six isolates presented phenotypic and genotypic characteristics of safety. Also, the antibacterial peptides present in the CFS showed stability at different temperatures, pH, NaCl and cure salt concentrations, as well as potential against L. monocytogenes Scott A. The isolate identified as Enterococcus faecium EO1 showed gene coding for the production of enterocin A and produced bacteriocin after 6 h of growth (400 AU. mL 1). Based on this, the CFS of EO1 (5 and 10%) was applied in fresh mutton sausage, being significantly effective for control of L. monocytogenes during 21 days of refrigerated storage (4.07 log CFU. g 1). Therefore, the potential for preservation of food has been demonstrated through the use of CFS containing bacteriocin-like substances from E. faecium EO1 in fresh mutton sausage. |
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Palavras-Chave: |
Bioconservação; Derivado de carne. |
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Thesagro: |
Carne Tratada; Carneiro; Listeria Monocytogenes; Ovino. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02468naa a2200313 a 4500
001 2133982
005 2021-09-01
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.lwt.2021.110954$2DOI
100 1 $aCOSTA, R. J. da
245 $aCharacterization of Enterococcus faecium EO1 isolated from mutton and activity of bacteriocin-like substances in the control of Listeria monocytogenes in fresh mutton sausage.$h[electronic resource]
260 $c2021
520 $aThe aim of this study was to isolate and characterize bacteriocinogenic lactic acid bacteria (LAB) from mutton carcasses after slaughter. The study also evaluated the activity of bacteriocin-like substances (BLS) for control of Listeria monocytogenes in fresh mutton sausage. The cell-free supernatant (CFS) was obtained and its antimicrobial activity was quantified. The presence of genes encoding enterocin was evaluated by molecular techniques. Another aspect assessed was the action in situ of BLS produced by these isolates for control of Listeria monocytogenes in fresh mutton sausage, experimentally contaminated. Six isolates presented phenotypic and genotypic characteristics of safety. Also, the antibacterial peptides present in the CFS showed stability at different temperatures, pH, NaCl and cure salt concentrations, as well as potential against L. monocytogenes Scott A. The isolate identified as Enterococcus faecium EO1 showed gene coding for the production of enterocin A and produced bacteriocin after 6 h of growth (400 AU. mL 1). Based on this, the CFS of EO1 (5 and 10%) was applied in fresh mutton sausage, being significantly effective for control of L. monocytogenes during 21 days of refrigerated storage (4.07 log CFU. g 1). Therefore, the potential for preservation of food has been demonstrated through the use of CFS containing bacteriocin-like substances from E. faecium EO1 in fresh mutton sausage.
650 $aCarne Tratada
650 $aCarneiro
650 $aListeria Monocytogenes
650 $aOvino
653 $aBioconservação
653 $aDerivado de carne
700 1 $aSILVA, A. P. da
700 1 $aFONSECA, R. N. da
700 1 $aHÜBNER, S. de O.
700 1 $aNALERIO, E. S.
700 1 $aMARQUES, J. de L.
700 1 $aVITOLA, H. R. S.
700 1 $aSILVA, W. P. da
700 1 $aDUVAL, E. H.
700 1 $aFIORENTINI, A. M.
773 $tFood Science and Technology$gv. 141, 110954, Apr. 2021.
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Registro original: |
Embrapa Pecuária Sul (CPPSUL) |
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| Registros recuperados : 9 | |
| 3. |  | PURCINO, A. A. C.; PAIVA, E.; SILVA, M. R.; ANDRADE, S. R. M. Influence of Azospirillum inoculation and nitrogen supply on grain yield, and carbon: and nitrogen-assimilating enzymes in maize. Journal of Plant Nutrition, New York, v. 19, n. 7, p. 1045-1060, 1996.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 6. | | PURCINO, A. A. C.; SILVA, M. R.; ANDRADE, S. R. M.; BELELE, C. L.; PARENTONI, S. N. Grain filling in maize: the effect of nitrogen nutrition on the activities of nitrogen assimilating enzymes in the pedicel-placento-chalaza region. Revista Brasileira de Fisiologia Vegetal, Brasília, v. 11, p. 145, 1999. Suplemento. Edição dos resumos do 7º Congresso Brasileiro de Fisiologia Vegetal, 1999, Brasilia, DF.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
|  |
| 9. | | PURCINO, A. A. C.; SILVA, M. R. e; ANDRADE, S. R. M.; BELELE, C. L.; PARENTONI, S. N.; SANTOS, M. X. dos. Grain filling in maize: the effect of nitrogen nutrition on the activities of nitrogen assimilating enzymes in the pedicel-placento-chalaza region. Maydica, Bergamo, v. 45, n. 2, p. 95-103, 2000.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 9 | |
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