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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
07/05/2013 |
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Data da última atualização: |
17/01/2024 |
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Autoria: |
RODRIGUES, G. Q.; SILVA, C. M. G.; FAUSTINO, L. R.; BRUNO, J. B.; MAGALHÃES, D. M.; CAMPELLO, C. C.; FIGUEIREDO, J. R. |
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Título: |
Bovine serum albumin improves in vitro development of caprine preantral follicles. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Animal Reproduction, v. 7, n. 4, p. 382-388, Oct./Dec. 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This work investigated the effects of different protein supplements (fetal calf serum (FCS) and bovine serum albumin (BSA)) on the in vitro development of caprine preantral follicles. Preantral follicles (?150 µm) were isolated from ovarian cortex fragments and individually cultivated in ?-MEM medium in an incubator at 37°C for 24 days with 5% atmospheric CO2, and supplemented with either BSA at 1.25 or 3.0 mg/ml or FCS at 5 or 10%. An evaluation of follicular development was conducted based on survival rate, antrum formation, increase in follicular diameter, oocyte viability and obtainment of fully-grown oocytes. It was observed that from the 12th cultivation day, the percentage of surviving follicles under treatment with BSA at 3.0 mg/ml was greater than that of the other treatments (P < 0.05). As for follicular growth, although on the 6th day of cultivation FCS-treated follicles had a greater mean diameter (P < 0.05) when compared to those treated with BSA, on the last day of cultivation the mean diameter and antrum formation of follicles treated with BSA at 3.0 mg/ml were greater than those of follicles under other treatments (P < 0.05). With oocyte growth, the percentage of oocytes cultivated with BSA (3.0 mg/ml) that were destined for in vitro maturation (IVM; ?110 ?m diameter) was higher than that of other treatments. Moreover, under this treatment, 86% of oocytes presented a germinal vesicle and 14% restarted meiosis, out of which 3% were mature (metaphase II). In conclusion, supplementing cultivation medium with BSA at 3.0 mg/ml not only improves follicular development but also provides meioticallycompetent oocytes after in vitro cultivation of caprine preantral isolated follicles. MenosAbstract: This work investigated the effects of different protein supplements (fetal calf serum (FCS) and bovine serum albumin (BSA)) on the in vitro development of caprine preantral follicles. Preantral follicles (?150 µm) were isolated from ovarian cortex fragments and individually cultivated in ?-MEM medium in an incubator at 37°C for 24 days with 5% atmospheric CO2, and supplemented with either BSA at 1.25 or 3.0 mg/ml or FCS at 5 or 10%. An evaluation of follicular development was conducted based on survival rate, antrum formation, increase in follicular diameter, oocyte viability and obtainment of fully-grown oocytes. It was observed that from the 12th cultivation day, the percentage of surviving follicles under treatment with BSA at 3.0 mg/ml was greater than that of the other treatments (P < 0.05). As for follicular growth, although on the 6th day of cultivation FCS-treated follicles had a greater mean diameter (P < 0.05) when compared to those treated with BSA, on the last day of cultivation the mean diameter and antrum formation of follicles treated with BSA at 3.0 mg/ml were greater than those of follicles under other treatments (P < 0.05). With oocyte growth, the percentage of oocytes cultivated with BSA (3.0 mg/ml) that were destined for in vitro maturation (IVM; ?110 ?m diameter) was higher than that of other treatments. Moreover, under this treatment, 86% of oocytes presented a germinal vesicle and 14% restarted meiosis, out of which 3% were mature (metaphase ... Mostrar Tudo |
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Palavras-Chave: |
Folítulo pre-antral; Oócito; Soro-Albumina. |
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Thesagro: |
Albumina; Cabra; Caprino; Cultura in vitro; Maturação; Reprodução animal. |
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Categoria do assunto: |
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Marc: |
LEADER 02581naa a2200301 a 4500
001 1957247
005 2024-01-17
008 2010 bl uuuu u00u1 u #d
100 1 $aRODRIGUES, G. Q.
245 $aBovine serum albumin improves in vitro development of caprine preantral follicles.$h[electronic resource]
260 $c2010
520 $aAbstract: This work investigated the effects of different protein supplements (fetal calf serum (FCS) and bovine serum albumin (BSA)) on the in vitro development of caprine preantral follicles. Preantral follicles (?150 µm) were isolated from ovarian cortex fragments and individually cultivated in ?-MEM medium in an incubator at 37°C for 24 days with 5% atmospheric CO2, and supplemented with either BSA at 1.25 or 3.0 mg/ml or FCS at 5 or 10%. An evaluation of follicular development was conducted based on survival rate, antrum formation, increase in follicular diameter, oocyte viability and obtainment of fully-grown oocytes. It was observed that from the 12th cultivation day, the percentage of surviving follicles under treatment with BSA at 3.0 mg/ml was greater than that of the other treatments (P < 0.05). As for follicular growth, although on the 6th day of cultivation FCS-treated follicles had a greater mean diameter (P < 0.05) when compared to those treated with BSA, on the last day of cultivation the mean diameter and antrum formation of follicles treated with BSA at 3.0 mg/ml were greater than those of follicles under other treatments (P < 0.05). With oocyte growth, the percentage of oocytes cultivated with BSA (3.0 mg/ml) that were destined for in vitro maturation (IVM; ?110 ?m diameter) was higher than that of other treatments. Moreover, under this treatment, 86% of oocytes presented a germinal vesicle and 14% restarted meiosis, out of which 3% were mature (metaphase II). In conclusion, supplementing cultivation medium with BSA at 3.0 mg/ml not only improves follicular development but also provides meioticallycompetent oocytes after in vitro cultivation of caprine preantral isolated follicles.
650 $aAlbumina
650 $aCabra
650 $aCaprino
650 $aCultura in vitro
650 $aMaturação
650 $aReprodução animal
653 $aFolítulo pre-antral
653 $aOócito
653 $aSoro-Albumina
700 1 $aSILVA, C. M. G.
700 1 $aFAUSTINO, L. R.
700 1 $aBRUNO, J. B.
700 1 $aMAGALHÃES, D. M.
700 1 $aCAMPELLO, C. C.
700 1 $aFIGUEIREDO, J. R.
773 $tAnimal Reproduction$gv. 7, n. 4, p. 382-388, Oct./Dec. 2010.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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