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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
08/07/2025 |
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Data da última atualização: |
08/07/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DURÉ, L. M. M.; MASCARIN, G. M.; BETTIOL, W. |
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Afiliação: |
LAÍS MAYARA MELO DURÉ, UNIVERSIDADE ESTADUAL PAULISTA; GABRIEL MOURA MASCARIN, CNPMA; WAGNER BETTIOL, CNPMA. |
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Título: |
Optimization of endospore production by solid and liquid fermentation for the development of effective formulations of Bacillus velezensis-based products. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 56, n. 2, p. 1253-1261, 2025. |
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ISSN: |
1678-4405 |
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DOI: |
https://doi.org/10.1007/s42770-025-01626-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The objective of this study was to optimize the culture medium for enhanced production of Bacillus velezensis AP-3 endospores using both liquid and solid-state fermentation techniques. Additionally, the study aimed to assess the stability of endospores in powder formulations containing talc and potato starch, and to evaluate the growth-promoting effects of these formulations on bean plants. Further, we evaluated the inhibition of mycelial growth of Sclerotinia sclerotiorum and Fusarium oxysporum f. sp. lycopercisi by metabolites produced by this bacterial strain. The plant growth promotion assay evaluated these formulations across various application methods: seed treatment, sowing furrow application, and a combination of both. Adjustments to culture medium significantly influenced the endospore yield of B. velezensis. Solid-state fermentation in rice flour and potato starch yielded up to 2.06 × 108 and 1.82 × 108 CFU g− 1 after 7 days in a 60% moisture medium, respectively. Conversely, submerged fermentation in molasses + cottonseed flour medium produced 1.7 × 109 CFU mL− 1 (viable endospores) in 3 days. The powder formulations showed high stability, maintaining viability for up to 226 days at room temperature. This bacterial strain effectively inhibited the mycelial growth of target fungal pathogens and promoted bean plant growth, particularly by enhancing root development. These findings highlight the versatility of B. velezensis AP-3 in producing endospores through two fermentation methods, its extended shelf-life as a wettable powder formulation, and its efficacy both as biocontrol agent and plant growth promoter, contributing to a sustainable agriculture. MenosAbstract: The objective of this study was to optimize the culture medium for enhanced production of Bacillus velezensis AP-3 endospores using both liquid and solid-state fermentation techniques. Additionally, the study aimed to assess the stability of endospores in powder formulations containing talc and potato starch, and to evaluate the growth-promoting effects of these formulations on bean plants. Further, we evaluated the inhibition of mycelial growth of Sclerotinia sclerotiorum and Fusarium oxysporum f. sp. lycopercisi by metabolites produced by this bacterial strain. The plant growth promotion assay evaluated these formulations across various application methods: seed treatment, sowing furrow application, and a combination of both. Adjustments to culture medium significantly influenced the endospore yield of B. velezensis. Solid-state fermentation in rice flour and potato starch yielded up to 2.06 × 108 and 1.82 × 108 CFU g− 1 after 7 days in a 60% moisture medium, respectively. Conversely, submerged fermentation in molasses + cottonseed flour medium produced 1.7 × 109 CFU mL− 1 (viable endospores) in 3 days. The powder formulations showed high stability, maintaining viability for up to 226 days at room temperature. This bacterial strain effectively inhibited the mycelial growth of target fungal pathogens and promoted bean plant growth, particularly by enhancing root development. These findings highlight the versatility of B. velezensis AP-3 in producing endospores thr... Mostrar Tudo |
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Palavras-Chave: |
Bacillus velezensis. |
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Thesagro: |
Controle Biológico; Estimulante de Crescimento Vegetal. |
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Thesaurus Nal: |
Antibiosis; Biological control agents; Growth promotion; Liquid state fermentation; Solid state fermentation; Wettable powders. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02687naa a2200277 a 4500
001 2177165
005 2025-07-08
008 2025 bl uuuu u00u1 u #d
022 $a1678-4405
024 7 $ahttps://doi.org/10.1007/s42770-025-01626-9$2DOI
100 1 $aDURÉ, L. M. M.
245 $aOptimization of endospore production by solid and liquid fermentation for the development of effective formulations of Bacillus velezensis-based products.$h[electronic resource]
260 $c2025
520 $aAbstract: The objective of this study was to optimize the culture medium for enhanced production of Bacillus velezensis AP-3 endospores using both liquid and solid-state fermentation techniques. Additionally, the study aimed to assess the stability of endospores in powder formulations containing talc and potato starch, and to evaluate the growth-promoting effects of these formulations on bean plants. Further, we evaluated the inhibition of mycelial growth of Sclerotinia sclerotiorum and Fusarium oxysporum f. sp. lycopercisi by metabolites produced by this bacterial strain. The plant growth promotion assay evaluated these formulations across various application methods: seed treatment, sowing furrow application, and a combination of both. Adjustments to culture medium significantly influenced the endospore yield of B. velezensis. Solid-state fermentation in rice flour and potato starch yielded up to 2.06 × 108 and 1.82 × 108 CFU g− 1 after 7 days in a 60% moisture medium, respectively. Conversely, submerged fermentation in molasses + cottonseed flour medium produced 1.7 × 109 CFU mL− 1 (viable endospores) in 3 days. The powder formulations showed high stability, maintaining viability for up to 226 days at room temperature. This bacterial strain effectively inhibited the mycelial growth of target fungal pathogens and promoted bean plant growth, particularly by enhancing root development. These findings highlight the versatility of B. velezensis AP-3 in producing endospores through two fermentation methods, its extended shelf-life as a wettable powder formulation, and its efficacy both as biocontrol agent and plant growth promoter, contributing to a sustainable agriculture.
650 $aAntibiosis
650 $aBiological control agents
650 $aGrowth promotion
650 $aLiquid state fermentation
650 $aSolid state fermentation
650 $aWettable powders
650 $aControle Biológico
650 $aEstimulante de Crescimento Vegetal
653 $aBacillus velezensis
700 1 $aMASCARIN, G. M.
700 1 $aBETTIOL, W.
773 $tBrazilian Journal of Microbiology$gv. 56, n. 2, p. 1253-1261, 2025.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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