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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
30/01/2025 |
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Data da última atualização: |
30/01/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RIBEIRO, A. C. S.; SANTOS, J. D. R.; GUERRERO-GUTIÉRREZ, M. M.; CUNHA, C. C. C.; SILVA, S. R.; BALARO, M. F. A.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; UNGERFELD, R.; BRANDÃO, F. Z. |
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Afiliação: |
UNIVERSIDADE FEDERAL FLUMINENSE; UNIVERSIDADE FEDERAL FLUMINENSE; UNIVERSIDADE FEDERAL FLUMINENSE; UNIVERSIDADE FEDERAL FLUMINENSE; UNIVERSIDADE FEDERAL FLUMINENSE; UNIVERSIDADE FEDERAL FLUMINENSE; UNIVERSIDADE FEDERAL FLUMINENSE; UNIVERSIDADE FEDERAL FLUMINENSE; JEFERSON FERREIRA DA FONSECA, CNPC; UNIVERSIDADE FEDERAL FLUMINENSE; UNIVERSIDADE FEDERAL FLUMINENSE. |
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Título: |
Reducing one third the dose of porcine follicle-stimulating hormone did not affect the in vivo embryo production in Santa Inês ewes. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Animal Production Science, v. 65, n. 2, AN24368, 2025. |
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DOI: |
https://doi.org/10.1071/AN24368 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Context - Superovulation is still a main limitation of multiple ovulation and embryo transfer programs because of its high cost and the variability in the responses to the hormonal protocols used. Aims - This study aimed to compare the effect of two doses of porcine follicle-stimulating hormone (pFSH) on follicular growth, superovulatory response, and in vivo embryo production of Santa Inês ewes. Methods - A total of 40 multiparous ewes received a Day 0 estrus synchronization protocol and were superovulated with either 333 IU (G333, n = 20) or 500 IU (G500, n = 20) of pFSH. Ultrasonographic evaluations of ovaries were performed every 12 h, from the first pFSH dose until 60 h after the last dose. Ewes were naturally mated and submitted to non-surgical embryo recovery. A subsample of viable blastocysts (G333: n = 10; G500: n = 8) was stained with Nile Red to quantify lipid droplets. Key results - The number of small, medium, and large follicles, and the size of the largest follicle, varied over time (P < 0.0001), even though there was no difference between the treatments. The pFSH dose also did not affect estrous behavior rate (95% vs 100%), superovulatory response (75% vs 70%), recovery rate (52.4% vs 56.4%), viability rate (73.8% vs 62.3%), recovered structures (5.9 vs 5.3), viable embryos (4.9 vs 3.7), and lipid accumulation in embryos (132.9 vs 129.4 arbitrary fluorescence units), for G333 and G500 respectively. Four ewes (10%) had premature luteal regression. It should be noted that all viable embryos recovered were Grade 1 or 2, that is, freezable embryos. Conclusions - In conclusion, 333 IU of pFSH is enough to reach a similar superovulatory response as obtained from 500 IU of pFSH in Santa Inês ewes. Implications - The use of lower doses of pFSH could reduce costs per embryo produced. MenosAbstract: Context - Superovulation is still a main limitation of multiple ovulation and embryo transfer programs because of its high cost and the variability in the responses to the hormonal protocols used. Aims - This study aimed to compare the effect of two doses of porcine follicle-stimulating hormone (pFSH) on follicular growth, superovulatory response, and in vivo embryo production of Santa Inês ewes. Methods - A total of 40 multiparous ewes received a Day 0 estrus synchronization protocol and were superovulated with either 333 IU (G333, n = 20) or 500 IU (G500, n = 20) of pFSH. Ultrasonographic evaluations of ovaries were performed every 12 h, from the first pFSH dose until 60 h after the last dose. Ewes were naturally mated and submitted to non-surgical embryo recovery. A subsample of viable blastocysts (G333: n = 10; G500: n = 8) was stained with Nile Red to quantify lipid droplets. Key results - The number of small, medium, and large follicles, and the size of the largest follicle, varied over time (P < 0.0001), even though there was no difference between the treatments. The pFSH dose also did not affect estrous behavior rate (95% vs 100%), superovulatory response (75% vs 70%), recovery rate (52.4% vs 56.4%), viability rate (73.8% vs 62.3%), recovered structures (5.9 vs 5.3), viable embryos (4.9 vs 3.7), and lipid accumulation in embryos (132.9 vs 129.4 arbitrary fluorescence units), for G333 and G500 respectively. Four ewes (10%) had premature luteal regression. It... Mostrar Tudo |
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Palavras-Chave: |
Crescimento folicular; Embryo collection; Embryonic quality; Follicular growth; Lipid quantification; Multiple ovulation. |
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Thesaurus Nal: |
Estrus synchronization; Follicle-stimulating hormone; Reproduction; Sheep; Superovulation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03046naa a2200385 a 4500
001 2172145
005 2025-01-30
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1071/AN24368$2DOI
100 1 $aRIBEIRO, A. C. S.
245 $aReducing one third the dose of porcine follicle-stimulating hormone did not affect the in vivo embryo production in Santa Inês ewes.$h[electronic resource]
260 $c2025
520 $aAbstract: Context - Superovulation is still a main limitation of multiple ovulation and embryo transfer programs because of its high cost and the variability in the responses to the hormonal protocols used. Aims - This study aimed to compare the effect of two doses of porcine follicle-stimulating hormone (pFSH) on follicular growth, superovulatory response, and in vivo embryo production of Santa Inês ewes. Methods - A total of 40 multiparous ewes received a Day 0 estrus synchronization protocol and were superovulated with either 333 IU (G333, n = 20) or 500 IU (G500, n = 20) of pFSH. Ultrasonographic evaluations of ovaries were performed every 12 h, from the first pFSH dose until 60 h after the last dose. Ewes were naturally mated and submitted to non-surgical embryo recovery. A subsample of viable blastocysts (G333: n = 10; G500: n = 8) was stained with Nile Red to quantify lipid droplets. Key results - The number of small, medium, and large follicles, and the size of the largest follicle, varied over time (P < 0.0001), even though there was no difference between the treatments. The pFSH dose also did not affect estrous behavior rate (95% vs 100%), superovulatory response (75% vs 70%), recovery rate (52.4% vs 56.4%), viability rate (73.8% vs 62.3%), recovered structures (5.9 vs 5.3), viable embryos (4.9 vs 3.7), and lipid accumulation in embryos (132.9 vs 129.4 arbitrary fluorescence units), for G333 and G500 respectively. Four ewes (10%) had premature luteal regression. It should be noted that all viable embryos recovered were Grade 1 or 2, that is, freezable embryos. Conclusions - In conclusion, 333 IU of pFSH is enough to reach a similar superovulatory response as obtained from 500 IU of pFSH in Santa Inês ewes. Implications - The use of lower doses of pFSH could reduce costs per embryo produced.
650 $aEstrus synchronization
650 $aFollicle-stimulating hormone
650 $aReproduction
650 $aSheep
650 $aSuperovulation
653 $aCrescimento folicular
653 $aEmbryo collection
653 $aEmbryonic quality
653 $aFollicular growth
653 $aLipid quantification
653 $aMultiple ovulation
700 1 $aSANTOS, J. D. R.
700 1 $aGUERRERO-GUTIÉRREZ, M. M.
700 1 $aCUNHA, C. C. C.
700 1 $aSILVA, S. R.
700 1 $aBALARO, M. F. A.
700 1 $aBATISTA, R. I. T. P.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aFONSECA, J. F. da
700 1 $aUNGERFELD, R.
700 1 $aBRANDÃO, F. Z.
773 $tAnimal Production Science$gv. 65, n. 2, AN24368, 2025.
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| 1. |  | EASTWOOD, R. J.; TAMBAM, B. B.; ABOAGYE, L. M.; AKPAROV, Z. I.; ALADELE, S. E.; ALLEN, R.; AMRI, A.; ANGLIN, N.; ARAYA, R.; ARRIETA-ESPINOZA, G.; ASGEROV, A.; AWANG, K.; AWAS, T.; BARATA, A. M.; BOATENG, S. K.; BREHM, J. M.; BREIDY, J.; BREMAN, E.; ANGULO, A. B.; BURLE, M. L.; CASTAÑEDA-ÁLVAREZ, N. P.; CASIMIRO, P.; CHAVES, N. F.; CLEMENTE, A. S.; COCKEL, C. P.; DAVEY, A.; LA ROSA, L. de; DEBOUCK, D. G.; DEMPEWOLF, H.; DOKMAK, H.; ELLIS, D.; FARUK, A.; FREITAS, C.; GALSTYAN, S.; GARCÍA, R. M.; GHIMIRE, K. H.; GUARINO, L.; HARKER, R.; HOPE, R.; HUMPHRIES, A.; JAMORA, N.; JATOI, S. A.; KHUTSISHVILI, M.; KIKODZE, D.; KYRATZIS, A. C.; LEÓN-LOBOS, P.; LIU, U.; MAINALI, R. P.; MAMMADOV, A. T.; MANRIQUE-CARPINTERO, N. C.; MANZELLA, D.; ALI, M. S. M.; MEDEIROS, M. B. de; GUZMÁN, M. A. M.; MIKATADZE-PANTSULAIA, T.; MOHAMED, E. T. I.; MONTEROS-ALTAMIRANO, A.; MORALES, A.; MÜLLER, J. V.; MULUMBA, J. W.; NERSESYAN, A.; NÓBREGA, H.; NYAMONGO, D. O; OBREZA, M.; OKERE, A. U.; ORSENIGO, S.; ORTEGA-KLOSE, F.; PAPIKYAN, A.; PEARCE, T. R.; CARVALHO, M. A. A. P. de; PROHENS, J.; ROSSI, G.; SALAS, A.; SHRESTHA, D. S.; SIDDIQUI, S. U.; SMITH, P. P.; SOTOMAYOR, D. A.; TACÁN, M.; TAPIA, C.; TOLEDO, A.; TOLL, J.; VU, D. T.; VU, T. D.; WAY, M. J.; YAZBEK, M.; ZORRILLA, C.; KILIAN, B. Adapting agriculture to climate change: a synopsis of Coordinated National Crop Wild Relative Seed Collecting Programs across five continents. Plants, v. 11, 2022. 1840. Na publicação: Marcelo B. Medeiros.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
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