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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
05/09/2022 |
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Data da última atualização: |
06/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MEDEIROS, F. L. V.; SANTOS, C. A. F.; COSTA, A. E. S. |
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Afiliação: |
F. L. B. MEDEIROS, UFRPE; CARLOS ANTONIO FERNANDES SANTOS, CPATSA; A. E. S. COSTA, UFRPE. |
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Título: |
Comparing SNP-based genetic linkage and physical maps in guava (Psidium guajava). |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 21, n. 2, gmr19033, 2022. |
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DOI: |
DOI http://dx.doi.org/10.4238/gmr19033 |
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Idioma: |
Inglês |
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Conteúdo: |
Genome mapping is a simplified representation of molecular markers or nucleotide sequences in chromosomes; developing accurate and dense maps is crucial for marker-assisted selection. We developed and compared genetic linkage maps obtained using JoinMap 4.0 and GACD with a physical map obtained using BLAST analysis based on Eucalyptus SNPs transferred to guava, Psidium guajava (Myrtaceae) - to serve as a reference for trait mapping in this crop. Genotyping was conducted on 112 individuals from an experimental cross between a well-known commercial cultivar and an exotic genotype (Pedro Sato × Purple guava), using the Euchip60K SNP chip, version 2.0 (72,202 SNPs); 79% of the SNPs were monomorphic. After data filtering, 1120 markers were used for map construction. The JoinMap 4.0 linkage map had 203 markers, spanning 1405.2 cM, with an average marker distance of 7.7 cM. The GACD linkage map had 186 markers and spanned 1392.7 cM, with an average marker distance of 8.8 cM. JoinMap and GACD disagreed on the estimated distances and SNP ordering. GACD showed a greater limitation than JoinMap 4.0 as it ordered markers according to their parental origin. The physical map developed using BLAST consisted of 694 hits (e-values from 8xE-10 to 1.15xE-26), spanning 434.88 Mb, with an average marker interval of 0.62 Mb. Both linkage maps showed linkage groups with segments from several chromosomes compared to the physical map, indicating limitations. These results highlight the effectiveness of physical mapping through BLAST to overcome linkage mapping limitations, such as in marker grouping and ordering. The physical maps proposed here can serve as a reference for mapping and QTL estimates in guava. MenosGenome mapping is a simplified representation of molecular markers or nucleotide sequences in chromosomes; developing accurate and dense maps is crucial for marker-assisted selection. We developed and compared genetic linkage maps obtained using JoinMap 4.0 and GACD with a physical map obtained using BLAST analysis based on Eucalyptus SNPs transferred to guava, Psidium guajava (Myrtaceae) - to serve as a reference for trait mapping in this crop. Genotyping was conducted on 112 individuals from an experimental cross between a well-known commercial cultivar and an exotic genotype (Pedro Sato × Purple guava), using the Euchip60K SNP chip, version 2.0 (72,202 SNPs); 79% of the SNPs were monomorphic. After data filtering, 1120 markers were used for map construction. The JoinMap 4.0 linkage map had 203 markers, spanning 1405.2 cM, with an average marker distance of 7.7 cM. The GACD linkage map had 186 markers and spanned 1392.7 cM, with an average marker distance of 8.8 cM. JoinMap and GACD disagreed on the estimated distances and SNP ordering. GACD showed a greater limitation than JoinMap 4.0 as it ordered markers according to their parental origin. The physical map developed using BLAST consisted of 694 hits (e-values from 8xE-10 to 1.15xE-26), spanning 434.88 Mb, with an average marker interval of 0.62 Mb. Both linkage maps showed linkage groups with segments from several chromosomes compared to the physical map, indicating limitations. These results highlight the effectiveness... Mostrar Tudo |
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Palavras-Chave: |
BLAST; Mapeamento de características qualitativas e quantitativas em goiaba; Transferibilidade de SNPs. |
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Thesagro: |
Genoma; Goiaba; Marcador Molecular; Melhoramento Genético Vegetal; Psidium Guajava. |
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Thesaurus Nal: |
Guavas. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02565naa a2200265 a 4500
001 2146109
005 2023-01-06
008 2022 bl uuuu u00u1 u #d
024 7 $aDOI http://dx.doi.org/10.4238/gmr19033$2DOI
100 1 $aMEDEIROS, F. L. V.
245 $aComparing SNP-based genetic linkage and physical maps in guava (Psidium guajava).$h[electronic resource]
260 $c2022
520 $aGenome mapping is a simplified representation of molecular markers or nucleotide sequences in chromosomes; developing accurate and dense maps is crucial for marker-assisted selection. We developed and compared genetic linkage maps obtained using JoinMap 4.0 and GACD with a physical map obtained using BLAST analysis based on Eucalyptus SNPs transferred to guava, Psidium guajava (Myrtaceae) - to serve as a reference for trait mapping in this crop. Genotyping was conducted on 112 individuals from an experimental cross between a well-known commercial cultivar and an exotic genotype (Pedro Sato × Purple guava), using the Euchip60K SNP chip, version 2.0 (72,202 SNPs); 79% of the SNPs were monomorphic. After data filtering, 1120 markers were used for map construction. The JoinMap 4.0 linkage map had 203 markers, spanning 1405.2 cM, with an average marker distance of 7.7 cM. The GACD linkage map had 186 markers and spanned 1392.7 cM, with an average marker distance of 8.8 cM. JoinMap and GACD disagreed on the estimated distances and SNP ordering. GACD showed a greater limitation than JoinMap 4.0 as it ordered markers according to their parental origin. The physical map developed using BLAST consisted of 694 hits (e-values from 8xE-10 to 1.15xE-26), spanning 434.88 Mb, with an average marker interval of 0.62 Mb. Both linkage maps showed linkage groups with segments from several chromosomes compared to the physical map, indicating limitations. These results highlight the effectiveness of physical mapping through BLAST to overcome linkage mapping limitations, such as in marker grouping and ordering. The physical maps proposed here can serve as a reference for mapping and QTL estimates in guava.
650 $aGuavas
650 $aGenoma
650 $aGoiaba
650 $aMarcador Molecular
650 $aMelhoramento Genético Vegetal
650 $aPsidium Guajava
653 $aBLAST
653 $aMapeamento de características qualitativas e quantitativas em goiaba
653 $aTransferibilidade de SNPs
700 1 $aSANTOS, C. A. F.
700 1 $aCOSTA, A. E. S.
773 $tGenetics and Molecular Research$gv. 21, n. 2, gmr19033, 2022.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Biblioteca |
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| Registros recuperados : 9 | |
| 2. |  | BERTOLOTE, L. E.; CAMPANA, M.; OLIVEIRA, P. P. A.; MORAIS, J. P. G. de; GODOY, R. Produção de forragem e qualidade de genótipos de aveia sobressemeadas em capim-tanzânia. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 45., 2008, Lavras, MG. Anais... Lavras: SBZ: UFLA, 2008. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 3. | | RIBEIRO, F. A.; BERNARDI, A. C. de C.; ESTEVES, S. N.; MORAIS, J. P. G. de; CARDOSO, R. D. Desaparecimento do nitrogênio amoniacal em vacas holandesas fistuladas alimentadas com zeólita e uréia. In: JORNADA Científica - São Carlos, 2., 2010, São Carlos, SP. Anais ... São Carlos, SP: Embrapa Pecuária Sudeste: Embrapa Instrumentação Agropecuária, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 5. | | CAMPANA, M.; BERTOLOTE, L. E. M.; OLIVEIRA, P. P. A.; MORAIS, J. P. G. de; BERNARDI, A. C. de C. Efeito residual do nitrogênio no sistema solo-planta sobre a produtividade do capim-tanzânia submetido a formas de aplicação e a fontes de adubo nitrogenado. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 45., 2008, Lavras, MG. Anais... Lavras: SBZ: UFLA, 2008. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 7. | | CAMPANA, M.; ALVES, A. C.; OLIVEIRA, P. P. A.; BERNARDI, A. C. de C.; SANTOS, E. A.; HERLING, V. R.; MORAIS, J. P. G. de; BARIONI JUNIOR, W. Ammonia volatilization from exposed soil and tanzania grass pasture fertilized with urea and zeolite mixture. Communications in Soil Science and Plant Analysis, v. 46, n. 8, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 8. | | RIBEIRO, F. A.; MORAIS, J. P. G. de; CARDOSO, R. D.; SOUZA, G. B. de; ESTEVES, S. N.; BERNARDI, A. C. de C. Digestibilidade in situ do sorgo e desaparecimento do Nitrogênio amoniacal em vacas holandesas alimentadas com Zeólita e Uréia. Veterinária e Zootecnia, v. 21, n. 1, p. 102-107, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 9. | | SILVA, S. C. da; BUENO, A. A. de O.; CARNEVALLI, R. A.; UEBELE, M. C.; BUENO, F. O.; HODGSON, J.; MATTHEW, C.; ARNOLD, G. C.; MORAIS, J. P. G. de. Sward structural characteristics and herbage accumulation of Panicum maximum cv. Mombaça subjected to rotational stocking managements. Scientia Agrícola, v. 66, n. 1, p. 8-19, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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