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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
10/06/2022 |
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Data da última atualização: |
11/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ZANELLA, L. B.; DEGENHARDT, J.; STEINER, N.; TOMASI, J.; KESTRING, D. R.; QUOIRIN, M. |
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Afiliação: |
LAUDIANE BRUNA ZANELLA, Federal University of Parana; JULIANA DEGENHARDT GOLDBACH, CNPF; NEUSA STEINER, Federal University of Santa Catarina; JÉSSICA TOMASI, Federal University of Parana; DAIANE RIGONI, CNPF; MARGUERITE QUOIRIN, Federal University of Parana. |
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Título: |
Initiation of somatic embryogenesis in Pinus caribaea var. hondurensis using mature female gametophytes as explants. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
South African Journal of Botany, v. 149, p. 124-133, 2022. |
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DOI: |
https://doi.org/10.1016/j.sajb.2022.05.053 |
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Idioma: |
Português |
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Conteúdo: |
ABSTRACT: Due to the difficulties of harvesting immature cones in genetic breeding orchards, such as high costs and the considerable time required, this study aimed to evaluate the somatic embryogenesis (SE) initiation of Pinus caribaea var. hondurensis using the mature female gametophyte as the initial explant. The basic medium for embryogenesis initiation was composed of QL (Quoirin and Lepoivre, 1977) nutrients, sucrose, myo-inositol, glutamine, casein hydrolisate, thiamine, 2,4-D, BAP and agar. This medium was compared with DCR (Gupta and Durzan, 1985) and WV5 (Coke, 1996) media, supplemented or not with 0.1 g L?1 folic acid (FA). Afterwards, three concentrations of 2,4-D (10, 20 and 30 ?M) combined or not with BAP (2, 4 and 8 ?M) were tested. After 90 days the percentages of explants with embryogenic tissue (ET), embryo-like structures and oxidation were evaluated. We observed somatic tissue initiation` on mature female gametophytes. On QL medium supplemented with FA, the initiation was observed on up to 42% of female gametophytes. The combination of 10 ?M 2,4-D and 4 ?M BAP resulted in the highest initiation rate. The formation of ET was confirmed in all treatments by a histochemical test. Embryo-like structures developed on QL medium supplemented with FA, were first transferred to basic medium supplemented with 1.5 g L?1 AC for 30 days and finally to the same medium supplemented with 10% polyethylene glycol 3350 and 120 µM abscisic acid. The expression of SERK-1 and GER-1 genes was analyzed. SERK-1 was expressed only in the ET, while GER-1 was expressed in both the embryogenic and non-embryogenic tissues (non-ET). However, the somatic embryos studied through morphological and histochemical tests proved to be abnormal and did not develop further. MenosABSTRACT: Due to the difficulties of harvesting immature cones in genetic breeding orchards, such as high costs and the considerable time required, this study aimed to evaluate the somatic embryogenesis (SE) initiation of Pinus caribaea var. hondurensis using the mature female gametophyte as the initial explant. The basic medium for embryogenesis initiation was composed of QL (Quoirin and Lepoivre, 1977) nutrients, sucrose, myo-inositol, glutamine, casein hydrolisate, thiamine, 2,4-D, BAP and agar. This medium was compared with DCR (Gupta and Durzan, 1985) and WV5 (Coke, 1996) media, supplemented or not with 0.1 g L?1 folic acid (FA). Afterwards, three concentrations of 2,4-D (10, 20 and 30 ?M) combined or not with BAP (2, 4 and 8 ?M) were tested. After 90 days the percentages of explants with embryogenic tissue (ET), embryo-like structures and oxidation were evaluated. We observed somatic tissue initiation` on mature female gametophytes. On QL medium supplemented with FA, the initiation was observed on up to 42% of female gametophytes. The combination of 10 ?M 2,4-D and 4 ?M BAP resulted in the highest initiation rate. The formation of ET was confirmed in all treatments by a histochemical test. Embryo-like structures developed on QL medium supplemented with FA, were first transferred to basic medium supplemented with 1.5 g L?1 AC for 30 days and finally to the same medium supplemented with 10% polyethylene glycol 3350 and 120 µM abscisic acid. The expression of SERK-1 and G... Mostrar Tudo |
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Palavras-Chave: |
Embryogenic tissues; GER-1; QL medium; SERK-1; Tecidos embriogênicos. |
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Thesagro: |
Ácido Fólico. |
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Thesaurus Nal: |
Folic acid; Pinus caribaea var. hondurensis. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02667naa a2200289 a 4500
001 2143947
005 2022-07-11
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.sajb.2022.05.053$2DOI
100 1 $aZANELLA, L. B.
245 $aInitiation of somatic embryogenesis in Pinus caribaea var. hondurensis using mature female gametophytes as explants.$h[electronic resource]
260 $c2022
520 $aABSTRACT: Due to the difficulties of harvesting immature cones in genetic breeding orchards, such as high costs and the considerable time required, this study aimed to evaluate the somatic embryogenesis (SE) initiation of Pinus caribaea var. hondurensis using the mature female gametophyte as the initial explant. The basic medium for embryogenesis initiation was composed of QL (Quoirin and Lepoivre, 1977) nutrients, sucrose, myo-inositol, glutamine, casein hydrolisate, thiamine, 2,4-D, BAP and agar. This medium was compared with DCR (Gupta and Durzan, 1985) and WV5 (Coke, 1996) media, supplemented or not with 0.1 g L?1 folic acid (FA). Afterwards, three concentrations of 2,4-D (10, 20 and 30 ?M) combined or not with BAP (2, 4 and 8 ?M) were tested. After 90 days the percentages of explants with embryogenic tissue (ET), embryo-like structures and oxidation were evaluated. We observed somatic tissue initiation` on mature female gametophytes. On QL medium supplemented with FA, the initiation was observed on up to 42% of female gametophytes. The combination of 10 ?M 2,4-D and 4 ?M BAP resulted in the highest initiation rate. The formation of ET was confirmed in all treatments by a histochemical test. Embryo-like structures developed on QL medium supplemented with FA, were first transferred to basic medium supplemented with 1.5 g L?1 AC for 30 days and finally to the same medium supplemented with 10% polyethylene glycol 3350 and 120 µM abscisic acid. The expression of SERK-1 and GER-1 genes was analyzed. SERK-1 was expressed only in the ET, while GER-1 was expressed in both the embryogenic and non-embryogenic tissues (non-ET). However, the somatic embryos studied through morphological and histochemical tests proved to be abnormal and did not develop further.
650 $aFolic acid
650 $aPinus caribaea var. hondurensis
650 $aÁcido Fólico
653 $aEmbryogenic tissues
653 $aGER-1
653 $aQL medium
653 $aSERK-1
653 $aTecidos embriogênicos
700 1 $aDEGENHARDT, J.
700 1 $aSTEINER, N.
700 1 $aTOMASI, J.
700 1 $aKESTRING, D. R.
700 1 $aQUOIRIN, M.
773 $tSouth African Journal of Botany$gv. 149, p. 124-133, 2022.
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Registro original: |
Embrapa Florestas (CNPF) |
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| Registros recuperados : 23 | |
| 3. |  | ANTONIOLI-LUIZON, R.; LOCALI-FABRIS, E. C.; ASTÚA, J. F.; MACHADO, M. A.; KITAJIMA, E. W. Estudo da variabilidade genética do vírus da pinta verde do maracujazeiro através de SSCP (Single Strand Conformational Polymorphism). In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 25., 2009, Porto de Galinhas, PE. Anais... [São Paulo]: Sociedade Bras. de Microbiologia, 2009. Resumo I - Virologia (Divisão P).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 6. | | PEREIRA, J. A.; ANTONIOLO-LUIZON, R.; LOCALI-FABRIS, E. C.; KUBO, K. S.; MACHADO, M. A.; FREITAS-ÁSTUA, J. Estudo da variabilidade genética da possível capa protéica do Citrus leprosis virus virus C (CiLV-C) em espécies de citros de 14 estados brasileiros. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 928.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 7. | | KITAJIMA, E. W.; CALEGARIO, R. F.; LOCALI-FABRIS, E. C.; NOVELLI, V. M.; BASTIANEL, M.; FREITAS-ASTÚA, J.; FRANCISCHINI, F. In situ detection of CiLV-C in the mite vector Brevipalpus phoenicis, evidence for a circulative type of virus/vector relationship and a model for the virus circulation in the mite. Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|  |
| 8. | | KITAJIMA, E. W.; CALEGARIO, R. F.; NOVELLI, V. M.; LOCALI-FABRIS, E. C.; BASTIANEL, M.; FRANCISCHINI, F.; FREITAS-ASTUA, J. In situ detection and immunolocalization of the Citrus leprosis virus cytoplasmic type (CiLV) in the mite vector and evidences that the virus/vector relationship is of circulative type. In: INTERNATIONAL SCIENTIFIC SEMINAR ON PLANT HEALTH, 6.; LATIN AMERICAN AND CARIBBEAN SYMPOSIUM, 2., 2008, La Habana, Cuba. Acarina Biodiversity: their use, protection and conservation. Havana, [s.n.], 2008. 1 CD ROM| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 9. | | NOVELLI, V. M.; ASTUA, J. de F.; LOCALI-FABRIS, E. C.; BASTIANEL, M.; ANTONIOLI-LUIZON, R.; RIBEIRO, B. M.; MACHADO, M. A. Detecção do vírus da leprose dos citros no ácaro vetor. Laranja, Cordeiropólis, v. 28, n. 1/2, 2007 Disponível em: <http://revistalaranja.centrodecitricultura.br/home.php?id=edicoes_revista&edicaon=5> Acesso em: 26 out. 2010. Pdf: 04_313 desenvolvimento [1]. pdf Secção: Fitopalogia| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 10. | | KUBO, K. S.; NOVELLI, V. M.; BASTIANEL, M.; LOCALI-FABRIS, E. C.; ANTONIOLI-LUIZON, R.; MACHADO, M. A.; FREITAS-ASTUA, J. Detection of Brevipalpus-transmitted viruses in their mite vectors by RT-PCR. Experimental Applied Acarology, Dordrechet, v. 54, n. 1, jan. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 11. | | FREITAS-ÁSTUA, J.; BASTIANEL, M.; LOCALI-FABRIS, E. C.; NOVELLI, V. M.; SILVA-PINHATI, A. C.; BASÍLIO-PALMIERI, A. C.; TARGON, M. L. P. N.; MACHADO, M. A. Differentially expressed stress-related genes in the compatible citrus-Citrus leprosis virus interaction. Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 980-990, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 12. | | NOVELLI, V. M.; FREITAS-ASTUA, J.; RIBEIRO, B. M.; LOCALI-FABRIS, E. C.; NAVIA, D.; KITAJIMA, E. W.; MACHADO, M. A. Prevalence of the Cardinium symbiont and genetic variability of Brevipalpus phoenicis (Acari: Tenuipalpidae) populations from Brazil. In:INTERNATIONAL CONGRESS OF ACAROLOGY, 13., 2010, Recife. Abstracts book... Recife: CNPq: FACEPE: Banco do Nordeste: TWAS, 2010. p. 181-182. Abstract 335| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 13. | | PEREIRA, J. A.; ANTONIOLI - LUIZON, R.; NICOLINI, F.; LOCALI-FABRIS, E. C.; KUBO, K. S.; M ACHADO, M. A.; FREITAS-ASTÚA, J. Análise da variabilidade genética do gene da capa protéica do vírus da leprose dos citros. Tropical Plant Pathology, Brasilia, DF, v. 33, supl., p. 287, ago. 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | LOCALI-FABRIS, E. C.; MAFRA, V. S.; MARTINS, P. K.; RIBEIRO- ALVES, M.; FRANCISCO, C. S.; FREITAS ASTUA, J. de; KISHI, L. T.; MACHADO, M. A. Gene set enrichment analysis in sweet orange plants infected by Candidatus Liberibacter americanus. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 0058 PSO Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 15. | | KUBO, K. S.; STUART, R. M.; FREITAS-ASTÚA, J.; ANTONIOLI-LUIZON, R.; LOCALI-FABRIS, E. C.; COLETTA-FILHO, H. D.; MACHADO, M. A.; KITAJIMA, E. W. Evaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and nucleotide sequencing of a fragment from the nucleocapsid. Archives of Virology, v. 154, n. 6, p. 1009-1014, mai. 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 16. | | NICOLINI, F.; BASTIANEL, M.; SCHONS, J.; KITAJIMA, E. W.; LOCALI-FABRIS, E. C.; PEREIRA, J. A.; KUBO, K. S.; NOVELLI, V. M.; MACHADO, M. A.; FREITAS-ASTÚA, J. Comparação entre os títulos de vírus transmitidos por ácaros Brevipalpus por RT-qPCR. Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 17. | | CALEGARIO, R. F.; KITAJIMA, E. W.; LOCALI-FABRIS, E. C.; SALAROLI, R. B.; FREITAS-ASTÚA, J.; STACH- MACHADO, D. R.; CASERTA, R.; MACHADO, M. A. In situ immunocytochemistry of citrus leaves infected with Citrus leprosis virus cytoplasmic type (CiLV-C). Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 18. | | FREITAS-ÁSTUA, J.; LOCALI-FABRIS, E. C.; ANTONIOLI-LUIZON, R.; PEREIRA, J.; NOVELLI, V. M.; CACERES, S.; LEON, M. G. A.; GOMEZ, E. C.; COSTA, F. M.; KITAJIMA, E. W.; MACHADO, M. A. Variabilidade do vírus da leprose dos citros C (CiLV-C) em amostras de diferentes países da América Latina. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 920.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 19. | | SOUZA, A. A. de; TAKITA, M. A.; COLETTA-FILHO, H. D.; TARGON, M. L. P. N.; CARLOS, E. F.; LOCALI-FABRIS, E. C.; AMARAL, A. M.; FREITAS-ÁSTUA, J.; SILVA-PINHATI, A. C. O.; BOSCARIOL-CAMARGO, R. L.; BERGER, I. J.; RODRIGUES, C. M.; REIS, M. S.; MACHADO, M. A. Analysis of expressed sequence tags from Citrus sinensis L. Osbeck infected with Xylella fastidiosa. Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 957-964, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 20. | | TARGON, M. L. P. N.; TAKITA, M. A.; AMARAL, A. M. do; SOUZA, A. A. de; LOCALI-FABRIS, E. C.; DORTA, S. de O.; BORGES, K. M.; SOUZA, J. M. de; RODRIGUES, C. M.; LUCHETA, A. R.; FREITAS-ÁSTUA, J.; MACHADO, M. A. CitEST libraries. Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 1019-1023, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 23 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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